CKA2/YOR061W Protein Information

Standard Name	Cka2p
Systematic Name	Yor061wp
Alias	Yor29-12p
ORF Classification	Verified
Description	Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching (1, 2, 3, 4, 5)
Name Description	Casein Kinase Alpha' subunit
Gene Product	CK2 subunit 1

Experimental Data
Molecules/cell	4540 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	339
Molecular Weight (Da)	39,403
Isoelectric Point (pI)	8.84

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cka2p (InterPro)
Physical Interactions	There are 216 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E77 \| P19454 MIPS: YOR061W NCBI: 125268 \| 1420203 \| 171233 \| 2104873 \| 51830522 \| 6324635 \| NP_014704.1 GenBank/EMBL/DDBJ: DAA10840.1 \| AY723867 \| M33759 \| Z70678 \| Z74969
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MPLPPSTLNQ KSNRVYSVAR VYKNACEERP QEYWDYEQGV TIDWGKISNY 51 EIINKIGRGK YSEVFSGRCI VNNQKCVIKV LKPVKMKKIY RELKILTNLT 101 GGPNVVGLYD IVQDADSKIP ALIFEEIKNV DFRTLYPTFK LPDIQYYFTQ 151 LLIALDYCHS MGIMHRDVKP QNVMIDPTER KLRLIDWGLA EFYHPGVDYN 201 VRVASRYHKG PELLVNLNQY DYSLDLWSVG CMLAAIVFKK EPFFKGSSNP 251 DQLVKIATVL GTKELLGYLG KYGLHLPSEY DNIMRDFTKK SWTHFITSET 301 KLAVPEVVDL IDNLLRYDHQ ERLTAKEAMD HKFFKTKFE*

Amino Acid Sequence (or in FASTA format)

       1  MPLPPSTLNQ KSNRVYSVAR VYKNACEERP QEYWDYEQGV TIDWGKISNY
      51  EIINKIGRGK YSEVFSGRCI VNNQKCVIKV LKPVKMKKIY RELKILTNLT
     101  GGPNVVGLYD IVQDADSKIP ALIFEEIKNV DFRTLYPTFK LPDIQYYFTQ
     151  LLIALDYCHS MGIMHRDVKP QNVMIDPTER KLRLIDWGLA EFYHPGVDYN
     201  VRVASRYHKG PELLVNLNQY DYSLDLWSVG CMLAAIVFKK EPFFKGSSNP
     251  DQLVKIATVL GTKELLGYLG KYGLHLPSEY DNIMRDFTKK SWTHFITSET
     301  KLAVPEVVDL IDNLLRYDHQ ERLTAKEAMD HKFFKTKFE*

external links for Cka2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cka2p

1)	Padmanabha R, et al. (1990) Isolation, sequencing, and disruption of the yeast CKA2 gene: casein kinase II is essential for viability in Saccharomyces cerevisiae. Mol Cell Biol 10(8):4089-99
2)	Ackermann K, et al. (2001) Genes targeted by protein kinase CK2: a genome-wide expression array analysis in yeast. Mol Cell Biochem 227(1-2):59-66
3)	Ahmed K, et al. (2002) Joining the cell survival squad: an emerging role for protein kinase CK2. Trends Cell Biol 12(5):226-30
4)	Li J, et al. (2012) Regulation of Budding Yeast Mating-Type Switching Donor Preference by the FHA Domain of Fkh1. PLoS Genet 8(4):e1002630
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41