ASE1/YOR058C Protein Information Help

Standard Name Ase1p
Systematic Name Yor058cp
Alias Yor29-09p
ORF Classification Verified
Description Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress (1, 2, 3, 4)
Name Description Anaphase Spindle Elongation 5
Experimental Data
Molecules/cell 556 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 885
Molecular Weight (Da) 101,623
Isoelectric Point (pI) 9.83

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ase1p (InterPro)
Physical Interactions There are 26 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001260F4 | P50275
MIPS: YOR058C
NCBI: 1420197 | 14318440 | 1703440 | 2104870 | 972942 | NP_116582.1
GenBank/EMBL/DDBJ: DAA10837.1 | U20235 | Z70678 | Z74966
Amino Acid Sequence (or in FASTA format)
 
       1  METATSSPLP IKSRRNSENS GSTTVIPHMN PSLATPLTVS TMVNQSNSKE
      51  FMKLTPVRIR DFGSPLKNVS TNYHFLDSEN GKGNTMDNMY RENFILISKD
     101  LEKLLENLNV IYQNIGYSNT EIITKEKIIF TTISNSIKQF FEQADEELKR
     151  LSAENGIEQD ILNNILERIN DPSGIKTIPD LYIRNAILLQ ESKTVPQSPK
     201  KPLSLLSKKA ALDTAKKFVL GSFLPRLRDY LKSLITLKHL IQSVKENLPG
     251  LTEADNEAIA EFPELSTLTA YLLQIENGKG DIGLSMKFII DNRKDILKGS
     301  AFKTINEESV KHMNEVIKIY EEEYERRFKS VLTKKVSISS ICEQLGTPLA
     351  TLIGEDFEQD LRSYGEEENS TSEIPNFHPV DRERMSKIDI TLEKLQAIHK
     401  ERADKKRLLM EQCQKLWTRL KISQEYIKTF MRNNSSLSTE SLGRISKEVM
     451  RLEAMKKKLI KKLISDSWDK IQELWRTLQY SEESRSKFII VFEELRNSAT
     501  TLQEDELLLE TCENELKRLE EKLTLYKPIL KLISDFESLQ EDQEFLERSS
     551  KDSSRLLSRN SHKILLTEEK MRKRITRHFP RVINDLRIKL EEADGLFDQP
     601  FLFKGKPLSE AIDIQQQEIE AKYPRCRVRM QRSKKGKCGA NKENKVIKNT
     651  FKATESSIRV PIGLNLNDAN ITYKTPSKKT IQGLTKNDLS QENSLARHMQ
     701  GTTKLSSPNR RATRLLAPTV ISRNSKGNIE RPTLNRNRSS DLSSSPRINH
     751  THGEHAVKPR QLFPIPLNKV DTKGSHIPQL TKEKALELLK RSTGTTGKEN
     801  VRSPERKSSL EDYAQKLSSP YKEPEHSIYK LSMSPEGKFQ LNIQQKDIES
     851  GFDDTSMMED ENDKDFITWK NEQVSKLNGF SFTDI*

external links for Ase1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
YGOB CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YOGY Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Ase1p
1) Juang YL, et al.  (1997) APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275(5304):1311-4
2) Schuyler SC, et al.  (2003) The molecular function of Ase1p: evidence for a MAP-dependent midzone-specific spindle matrix. Microtubule-associated proteins. J Cell Biol 160(4):517-28
3) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Pellman D, et al.  (1995) Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae. J Cell Biol 130(6):1373-85
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41