HIR2/YOR038C Protein Information

Standard Name	Hir2p
Systematic Name	Yor038cp
Alias	Spt1p 1
ORF Classification	Verified
Description	Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p (2, 3, 4, 5, 6, 7, 8)
Name Description	HIstone Regulation 5, 9

Experimental Data
Molecules/cell	922 10

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	875
Molecular Weight (Da)	98,444
Isoelectric Point (pI)	8.41

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hir2p (InterPro)
Physical Interactions	There are 44 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012C72E \| P32480 MIPS: YOR038C NCBI: 1041663 \| 1420160 \| 1708201 \| 476158 \| 6324612 \| NP_014681.1 GenBank/EMBL/DDBJ: DAA10821.1 \| L03839 \| U08849 \| X87331 \| Z74946

Amino Acid Sequence (or in FASTA format)
1 MRLLKYPLDI HNEQVNALAA LGPYIILAGS GGHVMAWRQQ QLVDTAFDRV 51 MIKDLKPEVS FQVDQDTTGD IFFITGDLET LYIGSEHRLW GYSGWLCRDT 101 NNINSVEKMN SKLLFECKSP STITDVKYDI NLGILFVLLS NENKILLFRH 151 KTFDKLSEIT IDKASKPITG IIDPTGQTFT VMTSDRSILV YQINKTGTHK 201 LINKLTQHVQ MYPLHYRISM SPQADILPVI NSVKGVPNNA TSCTALLDRN 251 NNYKVTKTLV TPSSNGCRVL VYSPAFYEKP NLKKGTSTRY NLIATSGSTD 301 GTILVWNTKR MKPLFNALQV SSTAINDMSW SQDGFTLFAI SNDATLYTFA 351 FQEKDLGVAL PQTEIKSLQE VNKKLPKLEE PLAEQIPKSF PENIKLEESA 401 SAAPIPNDIG RSAVGKKPTK KKTANNQTNG IKTIQSTSME FNTPSYTVPR 451 DLKRKPKEAT PSNIAPGSKK QKKELQPIDF LDTGLLLPNT SFSRIRLATP 501 KIRSTFKYSP INNPNLILDV KNGSGNEQRP TIVKLTSKVL DQDQVLFQDF 551 IPKLITICTA GDTFWSFCSE DGSIYIYSDS GRKLMAPLVL GVSISFLEAC 601 GTYLLCLTSI GELYCWNIEQ KKLAFPTNTI YPLLNPSLRY SDDILTRAEN 651 ITLCSITKKG VPLVTLSNGD GYLFDKNMET WLLVSDGWWA YGSQYWDTTN 701 TTGLSSSKAN TDSFNGSESN INEIVSDIKN DNQSIINFLE CKTNDELNRK 751 GRIKNLQRFA RTILMKEGFE NMEEIVTLSH LENKILISIR LEEPEEFSKL 801 MMVYCIRLSE LGYMDRLNDV FQWLYDDLPI SGTGSAFADK DFKRNLLKKI 851 LIACGDIRQV QRVTTRYAKE MNIIS*

Amino Acid Sequence (or in FASTA format)

       1  MRLLKYPLDI HNEQVNALAA LGPYIILAGS GGHVMAWRQQ QLVDTAFDRV
      51  MIKDLKPEVS FQVDQDTTGD IFFITGDLET LYIGSEHRLW GYSGWLCRDT
     101  NNINSVEKMN SKLLFECKSP STITDVKYDI NLGILFVLLS NENKILLFRH
     151  KTFDKLSEIT IDKASKPITG IIDPTGQTFT VMTSDRSILV YQINKTGTHK
     201  LINKLTQHVQ MYPLHYRISM SPQADILPVI NSVKGVPNNA TSCTALLDRN
     251  NNYKVTKTLV TPSSNGCRVL VYSPAFYEKP NLKKGTSTRY NLIATSGSTD
     301  GTILVWNTKR MKPLFNALQV SSTAINDMSW SQDGFTLFAI SNDATLYTFA
     351  FQEKDLGVAL PQTEIKSLQE VNKKLPKLEE PLAEQIPKSF PENIKLEESA
     401  SAAPIPNDIG RSAVGKKPTK KKTANNQTNG IKTIQSTSME FNTPSYTVPR
     451  DLKRKPKEAT PSNIAPGSKK QKKELQPIDF LDTGLLLPNT SFSRIRLATP
     501  KIRSTFKYSP INNPNLILDV KNGSGNEQRP TIVKLTSKVL DQDQVLFQDF
     551  IPKLITICTA GDTFWSFCSE DGSIYIYSDS GRKLMAPLVL GVSISFLEAC
     601  GTYLLCLTSI GELYCWNIEQ KKLAFPTNTI YPLLNPSLRY SDDILTRAEN
     651  ITLCSITKKG VPLVTLSNGD GYLFDKNMET WLLVSDGWWA YGSQYWDTTN
     701  TTGLSSSKAN TDSFNGSESN INEIVSDIKN DNQSIINFLE CKTNDELNRK
     751  GRIKNLQRFA RTILMKEGFE NMEEIVTLSH LENKILISIR LEEPEEFSKL
     801  MMVYCIRLSE LGYMDRLNDV FQWLYDDLPI SGTGSAFADK DFKRNLLKKI
     851  LIACGDIRQV QRVTTRYAKE MNIIS*

external links for Hir2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Hir2p

1)	Winston F, et al. (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97
2)	DeSilva H, et al. (1998) Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor. Genetics 148(2):657-67
3)	Kaufman PD, et al. (1998) Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I. Mol Cell Biol 18(8):4793-806
4)	Dimova D, et al. (1999) A role for transcriptional repressors in targeting the yeast Swi/Snf complex. Mol Cell 4(1):75-83
5)	Sherwood PW, et al. (1993) Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae. Mol Cell Biol 13(1):28-38
6)	Spector MS, et al. (1997) Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle. Mol Cell Biol 17(2):545-52
7)	Sharp JA, et al. (2001) Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing. Curr Biol 11(7):463-73
8)	Prochasson P, et al. (2005) The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Genes Dev 19(21):2534-9
9)	Osley MA and Lycan D (1987) Trans-acting regulatory mutations that alter transcription of Saccharomyces cerevisiae histone genes. Mol Cell Biol 7(12):4204-10
10)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41