BUB3/YOR026W Protein Information

Standard Name	Bub3p
Systematic Name	Yor026wp
Alias	Pac9p 1
ORF Classification	Verified
Description	Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p (2, 3, 4, 5)
Name Description	Budding Uninhibited by Benzimidazole 6

Experimental Data
Molecules/cell	1430 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	341
Molecular Weight (Da)	38,444
Isoelectric Point (pI)	4.93

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bub3p (InterPro)
Physical Interactions	There are 36 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000126B31 \| P26449 MIPS: YOR026W NCBI: 115150 \| 126030698 \| 126030700 \| 126030702 \| 126030704 \| 126030706 \| 126030708 \| 126030710 \| 1420137 \| 171137 \| 51247804 \| 51247805 \| 60594333 \| 6324600 \| 829136 \| NP_014669.1 GenBank/EMBL/DDBJ: DAA10808.1 \| M64707 \| X87331 \| Z74934

Amino Acid Sequence (or in FASTA format)
1 MQIVQIEQAP KDYISDIKII PSKSLLLITS WDGSLTVYKF DIQAKNVDLL 51 QSLRYKHPLL CCNFIDNTDL QIYVGTVQGE ILKVDLIGSP SFQALTNNEA 101 NLGICRICKY GDDKLIAASW DGLIEVIDPR NYGDGVIAVK NLNSNNTKVK 151 NKIFTMDTNS SRLIVGMNNS QVQWFRLPLC EDDNGTIEES GLKYQIRDVA 201 LLPKEQEGYA CSSIDGRVAV EFFDDQGDDY NSSKRFAFRC HRLNLKDTNL 251 AYPVNSIEFS PRHKFLYTAG SDGIISCWNL QTRKKIKNFA KFNEDSVVKI 301 ACSDNILCLA TSDDTFKTNA AIDQTIELNA SSIYIIFDYE N*

Amino Acid Sequence (or in FASTA format)

       1  MQIVQIEQAP KDYISDIKII PSKSLLLITS WDGSLTVYKF DIQAKNVDLL
      51  QSLRYKHPLL CCNFIDNTDL QIYVGTVQGE ILKVDLIGSP SFQALTNNEA
     101  NLGICRICKY GDDKLIAASW DGLIEVIDPR NYGDGVIAVK NLNSNNTKVK
     151  NKIFTMDTNS SRLIVGMNNS QVQWFRLPLC EDDNGTIEES GLKYQIRDVA
     201  LLPKEQEGYA CSSIDGRVAV EFFDDQGDDY NSSKRFAFRC HRLNLKDTNL
     251  AYPVNSIEFS PRHKFLYTAG SDGIISCWNL QTRKKIKNFA KFNEDSVVKI
     301  ACSDNILCLA TSDDTFKTNA AIDQTIELNA SSIYIIFDYE N*

external links for Bub3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Bub3p

1)	Geiser JR, et al. (1997) Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell 8(6):1035-50
2)	Martinez-Exposito MJ, et al. (1999) Retention of the BUB3 checkpoint protein on lagging chromosomes. Proc Natl Acad Sci U S A 96(15):8493-8
3)	Brady DM and Hardwick KG (2000) Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function. Curr Biol 10(11):675-8
4)	Hardwick KG, et al. (2000) MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p. J Cell Biol 148(5):871-82
5)	Fraschini R, et al. (2001) Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores. EMBO J 20(23):6648-59
6)	Hoyt MA, et al. (1991) S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell 66(3):507-17
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41