RTS1/YOR014W Protein Information

Standard Name	Rts1p 1
Systematic Name	Yor014wp
Alias	Scs1p 2
ORF Classification	Verified
Description	B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A (1, 3, 4, 5)
Name Description	Rox Three Suppressor 1

Experimental Data
Molecules/cell	300 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	757
Molecular Weight (Da)	85,334
Isoelectric Point (pI)	6.27

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rts1p (InterPro)
Physical Interactions	There are 44 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531BB \| P38903 MIPS: YOR014W NCBI: 1168144 \| 1420113 \| 1710806 \| 532526 \| 6324588 \| 829124 \| NP_014657.1 GenBank/EMBL/DDBJ: DAA10797.1 \| S79635 \| U06630 \| X87331 \| Z74922

Amino Acid Sequence (or in FASTA format)
1 MMRGFKQRLI KKTTGSSSSS SSKKKDKEKE KEKSSTTSST SKKPASASSS 51 SHGTTHSSAS STGSKSTTEK GKQSGSVPSQ GKHHSSSTSK TKTATTPSSS 101 SSSSRSSSVS RSGSSSTKKT SSRKGQEQSK QSQQPSQSQK QGSSSSSAAI 151 MNPTPVLTVT KDDKSTSGED HAHPTLLGAV SAVPSSPISN ASGTAVSSDV 201 ENGNSNNNNM NINTSNTQDA NHASSQSIDI PRSSHSFERL PTPTKLNPDT 251 DLELIKTPQR HSSSRFEPSR YTPLTKLPNF NEVSPEERIP LFIAKVDQCN 301 TMFDFNDPSF DIQGKEIKRS TLDELIEFLV TNRFTYTNEM YAHVVNMFKI 351 NLFRPIPPPV NPVGDIYDPD EDEPVNELAW PHMQAVYEFF LRFVESPDFN 401 HQIAKQYIDQ DFILKLLELF DSEDIRERDC LKTTLHRIYG KFLSLRSFIR 451 RSMNNIFLQF IYETEKFNGV AELLEILGSI INGFALPLKE EHKVFLVRIL 501 IPLHKVRCLS LYHPQLAYCI VQFLEKDPLL TEEVVMGLLR YWPKINSTKE 551 IMFLNEIEDI FEVIEPLEFI KVEVPLFVQL AKCISSPHFQ VAEKVLSYWN 601 NEYFLNLCIE NAEVILPIIF PALYELTSQL ELDTANGEDS ISDPYMLVEQ 651 AINSGSWNRA IHAMAFKALK IFLETNPVLY ENCNALYLSS VKETQQRKVQ 701 REENWSKLEE YVKNLRINND KDQYTIKNPE LRNSFNTASE NNTLNEENEN 751 DCDSEIQ*

Amino Acid Sequence (or in FASTA format)

       1  MMRGFKQRLI KKTTGSSSSS SSKKKDKEKE KEKSSTTSST SKKPASASSS
      51  SHGTTHSSAS STGSKSTTEK GKQSGSVPSQ GKHHSSSTSK TKTATTPSSS
     101  SSSSRSSSVS RSGSSSTKKT SSRKGQEQSK QSQQPSQSQK QGSSSSSAAI
     151  MNPTPVLTVT KDDKSTSGED HAHPTLLGAV SAVPSSPISN ASGTAVSSDV
     201  ENGNSNNNNM NINTSNTQDA NHASSQSIDI PRSSHSFERL PTPTKLNPDT
     251  DLELIKTPQR HSSSRFEPSR YTPLTKLPNF NEVSPEERIP LFIAKVDQCN
     301  TMFDFNDPSF DIQGKEIKRS TLDELIEFLV TNRFTYTNEM YAHVVNMFKI
     351  NLFRPIPPPV NPVGDIYDPD EDEPVNELAW PHMQAVYEFF LRFVESPDFN
     401  HQIAKQYIDQ DFILKLLELF DSEDIRERDC LKTTLHRIYG KFLSLRSFIR
     451  RSMNNIFLQF IYETEKFNGV AELLEILGSI INGFALPLKE EHKVFLVRIL
     501  IPLHKVRCLS LYHPQLAYCI VQFLEKDPLL TEEVVMGLLR YWPKINSTKE
     551  IMFLNEIEDI FEVIEPLEFI KVEVPLFVQL AKCISSPHFQ VAEKVLSYWN
     601  NEYFLNLCIE NAEVILPIIF PALYELTSQL ELDTANGEDS ISDPYMLVEQ
     651  AINSGSWNRA IHAMAFKALK IFLETNPVLY ENCNALYLSS VKETQQRKVQ
     701  REENWSKLEE YVKNLRINND KDQYTIKNPE LRNSFNTASE NNTLNEENEN
     751  DCDSEIQ*

external links for Rts1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rts1p

1)	Shu Y, et al. (1997) Molecular genetic analysis of Rts1p, a B' regulatory subunit of Saccharomyces cerevisiae protein phosphatase 2A. Mol Cell Biol 17(6):3242-53
2)	Shu Y and Hallberg RL (1995) SCS1, a multicopy suppressor of hsp60-ts mutant alleles, does not encode a mitochondrially targeted protein. Mol Cell Biol 15(10):5618-26
3)	Zhao Y, et al. (1997) Saccharomyces cerevisiae homologs of mammalian B and B' subunits of protein phosphatase 2A direct the enzyme to distinct cellular functions. J Biol Chem 272(13):8256-62
4)	Dobbelaere J, et al. (2003) Phosphorylation-dependent regulation of septin dynamics during the cell cycle. Dev Cell 4(3):345-57
5)	Bizzari F and Marston AL (2011) Cdc55 coordinates spindle assembly and chromosome disjunction during meiosis. J Cell Biol 193(7):1213-28
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41