AUS1/YOR011W Protein Information Help

Standard Name Aus1p 1
Systematic Name Yor011wp
ORF Classification Verified
Description Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen; AUS1 has a paralog, PDR11, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description ABC protein involved in Uptake of Sterols 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,394
Molecular Weight (Da) 157,750
Isoelectric Point (pI) 7.16

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Aus1p (InterPro)
Transmembrane Domains There are 13 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 43 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006B61D | Q08409
MIPS: YOR011W
NCBI: 1420108 | 2326810 | 51701315 | 6324585 | NP_014654.1 | NM_001183430.1
GenBank/EMBL/DDBJ: CAA99199.1 | CAA99202.1 | DAA10794.1 | Z74919 | Z74920
External Classifications TC: 3.A.1.205 [The Pleiotropic Drug Resistance (PDR) Family]
Amino Acid Sequence (or in FASTA format)
       1  MSISKYFTPV ADGSLTFNGA NIQFGADAQG ESKKSYDAED SMPNPANQLN
      51  DITFQAEAGE MVLVLGYPTS TLFKTLFHGK TSLSYSPPGS IKFKNNEFKS
     101  FSEKCPHQII YNNEQDVHFP FLTVEQTIDF ALSCKFDIPK GERDQIRNEL
     151  LREFGLSHVL KTIVGNDFFR GVSGGERKRI SIIETFIANG SVYLWDNSTK
     201  GLDSATALDF LEILRKMAKA TRSVNLVRIS QASDKIVDKF DKILMLSDSY
     251  QLFYGTVDEC LTYFRDTLGI EKDPNDCIIE YLTSILNFQF KNKNLGNLSN
     301  SSSASVLKTA TGEVTKYTYN SDFDLYDQWK HSSYYRNIKQ QIQGSSIDDS
     351  IKEVDPSDVS PIFNIPLKKQ LLFCTKRAFQ RSLGDKAYMT AQFISVVIQS
     401  LVIGSLFYEI PLTTIGSYSR GSLTFFSILF FTFLSLADMP IAFQRQPVVK
     451  KQSQLHFYTN WVETLSTTVF DYCFKLCLVI VFSIILYFLA HLQYKAARFF
     501  IFLLFLSFYN FCMVSLFALT TLVAPTISVA NLFAGILLLA IAMYASYVIY
     551  LKNMHPWFVW IAYLNPAMYA MEAILSNELY NLKLDCSETI VPRGPTYNDV
     601  PFSHKACAWQ GATLGNDYVR GRDYLKQGLS YTYHHVWRNF GIIIGFLVFF
     651  IACTLFASQY IKPYFNKDEI ERNNSRLTRW LPFLNKKRGT RSSARNDSKY
     701  VGIPKSHSVS SSSSSLSAVP YQISPSNKEM ALNDYNEQPI TETVETQKHI
     751  ISWKNINYTV GTKKLINNAS GFISSGLTAL MGESGAGKTT LLNVLSQRVE
     801  TGVVSGEILI DGHPLTDEDA FKRSIGFVQQ QDLHLDLLSV KESLEISCLL
     851  RGDGDRAYLD TVSNLLKLPS DILVADLNPT QRKLLSIGVE LVTKPSLLLF
     901  LDEPTSGLDA EAALTIVKFL KQLSLQGQAI FCTIHQPSKS VISHFDNIFL
     951  LKRGGECVFF GPMDDACGYF MSHDNTLVYD KEHDNPADFV IDAVGNSNSS
    1001  AGKDTAEEAL TLNKEAIDWS ALWESSVEKK LVKKETARLE DDARASGVDY
    1051  TTSLWKQPSY LQQLALITRR QYICTKRDMT YVMAKYCLNG GAGLFIGFSF
    1101  WHIKHNIIGL QDSIFFCFMA LCVSSPLINQ IQDKALKTKE VYVAREARSN
    1151  TYHWTVLLLS QSIIELPLAL TSSTLFFVCA FFSCGFNNAG WSAGVFFLNY
    1201  MLFAAYYSTL GLWLIYTAPN LQTAAVFVAF IYSFTASFCG VMQPYSLFPT
    1251  FWKFMYRVSP YTYFVETFVS ILLHNWEIKC DMSEMVPGQP LTGQSCGQFM
    1301  EAFIEEYGGY LHNKNTFTVC AYCTYTVGDD FLKNENMSYD HVWRNFGIEW
    1351  AFVGFNFFAM FAGYYLTYVA RIWPKVFKII TKVIPHRGKK PVQN*     

external links for Aus1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB CYC2008 (complexes) MIPS YeastGFP
YOGY Complexome Pfam domains

DIP TCDB

GeneMANIA YeastRC Structure Prediction (Seattle)

IMP

References cited on this page View Complete Literature Guide for Aus1p
1) Wilcox LJ, et al.  (2002) Transcriptional profiling identifies two members of the ATP-binding cassette transporter superfamily required for sterol uptake in yeast. J Biol Chem 277(36):32466-72
2) Alimardani P, et al.  (2004) SUT1-promoted sterol uptake involves the ABC transporter Aus1 and the mannoprotein Dan1 whose synergistic action is sufficient for this process. Biochem J 381(Pt 1):195-202
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Kohut P, et al.  (2011) The role of ABC proteins Aus1p and Pdr11p in the uptake of external sterols in yeast: Dehydroergosterol fluorescence study. Biochem Biophys Res Commun 404(1):233-8
5) Marek M, et al.  (2011) The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity. J Biol Chem 286(24):21835-43