PPM2/YOL141W Protein Information Help

Standard Name Ppm2p 1
Systematic Name Yol141wp
Alias Tyw4p 2
ORF Classification Verified
Description AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p (2, 3, 4, 5, 6)
Name Description Protein Phosphatase Methyltransferase 1
Experimental Data
Molecules/cell 830 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 695
Molecular Weight (Da) 78,956
Isoelectric Point (pI) 7.04

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ppm2p (InterPro)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0001A57B60 | Q08282
MIPS: YOL141W
NCBI: 1420038 | 238828116 | 238828117 | 238828118 | 238828119 | 238828120 | 238828121 | 330443721 | 347595662 | NP_014500.2
GenBank/EMBL/DDBJ: DAA10644.2 | Z74883
External Classifications EC: 2.1.1.- [Transferases transferring one-carbon groups methyltransferases]
Amino Acid Sequence (or in FASTA format)
 
       1  MKNLTTIKQT NKNVKQERRK KYADLAIQGT NNSSIASKRS VELLYLPKLS
      51  SANNFQMDKN NKLLEYFKFF VPKKIKRSPC INRGYWLRLF AIRSRLNSII
     101  EQTPQDKKIV VVNLGCGYDP LPFQLLDTNN IQSQQYHDRV SFIDIDYSDL
     151  LKIKIELIKT IPELSKIIGL SEDKDYVDDS NVDFLTTPKY LARPCDLNDS
     201  KMFSTLLNEC QLYDPNVVKV FVAEVSLAYM KPERSDSIIE ATSKMENSHF
     251  IILEQLIPKG PFEPFSKQML AHFKRNDSPL QSVLKYNTIE SQVQRFNKLG
     301  FAYVNVGDMF QLWESADEAT KKELLKVEPF DELEEFHLFC HHYVLCHATN
     351  YKEFAFTQGF LFDRSISEIN LTVDEDYQLL ECECPINRKF GDVDVAGNDV
     401  FYMGGSNPYR VNEILQLSIH YDKIDMKNIE VSSSEVPVAR MCHTFTTISR
     451  NNQLLLIGGR KAPHQGLSDN WIFDMKTREW SMIKSLSHTR FRHSACSLPD
     501  GNVLILGGVT EGPAMLLYNV TEEIFKDVTP KDEFFQNSLV SAGLEFDPVS
     551  KQGIILGGGF MDQTTVSDKA IIFKYDAENA TEPITVIKKL QHPLFQRYGS
     601  QIKYITPRKL LIVGGTSPSG LFDRTNSIIS LDPLSETLTS IPISRRIWED
     651  HSLMLAGFSL VSTSMGTIHI IGGGATCYGF GSVTNVGLKL IAIAK*

external links for Ppm2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB GeneMANIA

YOGY


References cited on this page View Complete Literature Guide for Ppm2p
1) Wu J, et al.  (2000) Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo. EMBO J 19(21):5672-81
2) Noma A, et al.  (2006) Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J 25(10):2142-54
3) Wei H, et al.  (2001) Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p. J Biol Chem 276(2):1570-7
4) Kalhor HR, et al.  (2001) Protein phosphatase methyltransferase 1 (Ppm1p) is the sole activity responsible for modification of the major forms of protein phosphatase 2A in yeast. Arch Biochem Biophys 395(2):239-45
5) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
6) Noma A and Suzuki T  (2006) Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp Ser (Oxf) (50):65-6
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41