PFK27/YOL136C Protein Information

Standard Name	Pfk27p 1
Systematic Name	Yol136cp
ORF Classification	Verified
Description	6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A (1, 2, 3, 4)
Name Description	6-PhosphoFructo-2-Kinase 1
Gene Product	PFK-2

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	397
Molecular Weight (Da)	45,317
Isoelectric Point (pI)	5.47

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pfk27p (InterPro)
Physical Interactions	There are 10 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000124F5C \| Q12471 MIPS: YOL136C NCBI: 1085116 \| 1420028 \| 1628439 \| 2499511 \| 6324436 \| NP_014505.1 \| NM_001183390.1 GenBank/EMBL/DDBJ: DAA10649.1 \| X90861 \| X95465 \| Z74878
External Classifications	EC: 2.7.1.105 [6-phosphofructo-2-kinase]

Amino Acid Sequence (or in FASTA format)
1 MGGSSDSDSH DGYLTSEYNS SNSLFSLNTG NSYSSASLDR ATLDCQDSVF 51 FDNHKSSLLS TEVPRFISND PLHLPITLNY KRDNADPTYT NGKVNKFMIV 101 LIGLPATGKS TISSHLIQCL KNNPLTNSLR CKVFNAGKIR RQISCATISK 151 PLLLSNTSSE DLFNPKNNDK KETYARITLQ KLFHEINNDE CDVGIFDATN 201 STIERRRFIF EEVCSFNTDE LSSFNLVPII LQVSCFNRSF IKYNIHNKSF 251 NEDYLDKPYE LAIKDFAKRL KHYYSQFTPF SLDEFNQIHR YISQHEEIDT 301 SLFFFNVINA GVVEPHSLNQ SHYPSTCGKQ IRDTIMVIEN FINHYSQMFG 351 FEYIEAVKLF FESFGNSSEE TLTTLDSVVN DKFFDDLQSL IESNGFA*

Amino Acid Sequence (or in FASTA format)

       1  MGGSSDSDSH DGYLTSEYNS SNSLFSLNTG NSYSSASLDR ATLDCQDSVF
      51  FDNHKSSLLS TEVPRFISND PLHLPITLNY KRDNADPTYT NGKVNKFMIV
     101  LIGLPATGKS TISSHLIQCL KNNPLTNSLR CKVFNAGKIR RQISCATISK
     151  PLLLSNTSSE DLFNPKNNDK KETYARITLQ KLFHEINNDE CDVGIFDATN
     201  STIERRRFIF EEVCSFNTDE LSSFNLVPII LQVSCFNRSF IKYNIHNKSF
     251  NEDYLDKPYE LAIKDFAKRL KHYYSQFTPF SLDEFNQIHR YISQHEEIDT
     301  SLFFFNVINA GVVEPHSLNQ SHYPSTCGKQ IRDTIMVIEN FINHYSQMFG
     351  FEYIEAVKLF FESFGNSSEE TLTTLDSVVN DKFFDDLQSL IESNGFA*

external links for Pfk27p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Pfk27p

1)	Boles E, et al. (1996) Cloning of a second gene encoding 5-phosphofructo-2-kinase in yeast, and characterization of mutant strains without fructose-2,6-bisphosphate. Mol Microbiol 20(1):65-76
2)	Goncalves PM, et al. (1997) Signalling pathways leading to transcriptional regulation of genes involved in the activation of glycolysis in yeast. Mol Microbiol 25(3):483-93
3)	Kretschmer M, et al. (1993) Mutation of monofunctional 6-phosphofructo-2-kinase in yeast to bifunctional 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase. Biochemistry 32(41):11143-8
4)	Bedri A, et al. (1989) Kinetics of 6-phosphofructo-2-kinase from Saccharomyces cerevisiae: inhibition of the enzyme by ATP. Biomed Biochim Acta 48(7):403-11