MED7/YOL135C Protein Information

Standard Name	Med7p 1
Systematic Name	Yol135cp
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 2)
Name Description	MEDiator complex 1

Experimental Data
Molecules/cell	7600 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	222
Molecular Weight (Da)	25,585
Isoelectric Point (pI)	6.94

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Med7p (InterPro)
Physical Interactions	There are 192 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053154 \| Q08278 MIPS: YOL135C NCBI: 1420026 \| 1628440 \| 218681922 \| 218681924 \| 218681926 \| 218681928 \| 48474785 \| 51013229 \| 61680694 \| 61680696 \| 61680698 \| 6324437 \| NP_014506.1 GenBank/EMBL/DDBJ: DAA10650.1 \| AY692889 \| X95465 \| Z74877

Amino Acid Sequence (or in FASTA format)
1 MSNDPGNEVS SLYPPPPPYV KFFTQSNLEK LPKYKEKKAA SAKQTAPNNS 51 NGGSEEEITC ALDYLIPPPM PKNQQYRAFG SIWQVKDQLP DLESMGLTQL 101 YKKSTENEST NYQYKIQELR KLLKSLLLNY LELIGVLSIN PDMYERKVEN 151 IRTILVNIHH LLNEYRPHQS RESLIMLLEE QLEYKRGEIR EIEQVCKQVH 201 DKLTSIQDTL RTGSQSPPSS SQ*

Amino Acid Sequence (or in FASTA format)

       1  MSNDPGNEVS SLYPPPPPYV KFFTQSNLEK LPKYKEKKAA SAKQTAPNNS
      51  NGGSEEEITC ALDYLIPPPM PKNQQYRAFG SIWQVKDQLP DLESMGLTQL
     101  YKKSTENEST NYQYKIQELR KLLKSLLLNY LELIGVLSIN PDMYERKVEN
     151  IRTILVNIHH LLNEYRPHQS RESLIMLLEE QLEYKRGEIR EIEQVCKQVH
     201  DKLTSIQDTL RTGSQSPPSS SQ*

external links for Med7p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Med7p

1)	Myers LC, et al. (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
2)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41