HRT1/YOL133W Protein Information Help

Standard Name Hrt1p 1
Systematic Name Yol133wp
Alias Rbx1p , Roc1p 2 , Hrt2p
ORF Classification Verified
Description RING-H2 domain core subunit of multiple ubiquitin ligase complexes; subunit of Skp1-Cullin-F-box (SCF) that tethers the Cdc34p (E2) and Cdc53p (cullin) SCF subunits, and is required for degradation of Gic2p, Far1p, Sic1p and Cln2p; subunit of the Rtt101p-Mms1p-Mms22p ubiquitin ligase that stabilizes replication forks after DNA lesions; subunit of the Cul3p-Elc1p-Ela1p ubiquitin ligase involved in Rpb1p degradation as part of transcription-coupled repair (1, 2, 3, 4, 5, 6, 7, 8)
Name Description High level expression Reduces Ty3 transposition 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 121
Molecular Weight (Da) 13,940
Isoelectric Point (pI) 5.64

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Hrt1p (InterPro)
Physical Interactions There are 107 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000069FBD | Q08273
MIPS: YOL133W
NCBI: 1420024 | 1628443 | 37537921 | 6324438 | NP_014508.1
GenBank/EMBL/DDBJ: DAA10651.1 | X95465 | Z74876
Amino Acid Sequence (or in FASTA format)
 
       1  MSNEVDRMDV DEDESQNIAQ SSNQSAPVET KKKRFEIKKW TAVAFWSWDI
      51  AVDNCAICRN HIMEPCIECQ PKAMTDTDNE CVAAWGVCNH AFHLHCINKW
     101  IKTRDACPLD NQPWQLARCG R*

external links for Hrt1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Hrt1p
1) Seol JH, et al.  (1999) Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes Dev 13(12):1614-26
2) Ohta T, et al.  (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol Cell 3(4):535-41
3) Blondel M, et al.  (2000) Isolation and characterization of HRT1 using a genetic screen for mutants unable to degrade Gic2p in saccharomyces cerevisiae. Genetics 155(3):1033-44
4) Kamura T, et al.  (1999) Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. Science 284(5414):657-61
5) Skowyra D, et al.  (1999) Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1. Science 284(5414):662-5
6) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
7) Michel JJ, et al.  (2003) A role for Saccharomyces cerevisiae Cul8 ubiquitin ligase in proper anaphase progression. J Biol Chem 278(25):22828-37
8) Finley D, et al.  (2012) The Ubiquitin-Proteasome System of Saccharomyces cerevisiae. Genetics 192(2):319-60