MDH2/YOL126C Protein Information

Standard Name	Mdh2p
Systematic Name	Yol126cp
ORF Classification	Verified
Description	Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 (1, 2, 3)
Name Description	Malate DeHydrogenase

Experimental Data
Molecules/cell	5260 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	377
Molecular Weight (Da)	40,730
Isoelectric Point (pI)	6.89

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mdh2p (InterPro)
Physical Interactions	There are 22 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000168CCF \| P22133 MIPS: YOL126C NCBI: 116006499 \| 1209715 \| 126895 \| 1420009 \| 171916 \| NP_014515.2 \| NM_001183380.1 GenBank/EMBL/DDBJ: DAA10658.1 \| M62808 \| U41293 \| Z74868
External Classifications	EC: 1.1.1.37 [Malate dehydrogenase]

Amino Acid Sequence (or in FASTA format)
1 MPHSVTPSIE QDSLKIAILG AAGGIGQSLS LLLKAQLQYQ LKESNRSVTH 51 IHLALYDVNQ EAINGVTADL SHIDTPISVS SHSPAGGIEN CLHNASIVVI 101 PAGVPRKPGM TRDDLFNVNA GIISQLGDSI AECCDLSKVF VLVISNPVNS 151 LVPVMVSNIL KNHPQSRNSG IERRIMGVTK LDIVRASTFL REINIESGLT 201 PRVNSMPDVP VIGGHSGETI IPLFSQSNFL SRLNEDQLKY LIHRVQYGGD 251 EVVKAKNGKG SATLSMAHAG YKCVVQFVSL LLGNIEQIHG TYYVPLKDAN 301 NFPIAPGADQ LLPLVDGADY FAIPLTITTK GVSYVDYDIV NRMNDMERNQ 351 MLPICVSQLK KNIDKGLEFV ASRSASS*

Amino Acid Sequence (or in FASTA format)

       1  MPHSVTPSIE QDSLKIAILG AAGGIGQSLS LLLKAQLQYQ LKESNRSVTH
      51  IHLALYDVNQ EAINGVTADL SHIDTPISVS SHSPAGGIEN CLHNASIVVI
     101  PAGVPRKPGM TRDDLFNVNA GIISQLGDSI AECCDLSKVF VLVISNPVNS
     151  LVPVMVSNIL KNHPQSRNSG IERRIMGVTK LDIVRASTFL REINIESGLT
     201  PRVNSMPDVP VIGGHSGETI IPLFSQSNFL SRLNEDQLKY LIHRVQYGGD
     251  EVVKAKNGKG SATLSMAHAG YKCVVQFVSL LLGNIEQIHG TYYVPLKDAN
     301  NFPIAPGADQ LLPLVDGADY FAIPLTITTK GVSYVDYDIV NRMNDMERNQ
     351  MLPICVSQLK KNIDKGLEFV ASRSASS*

external links for Mdh2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Mdh2p

1)	Minard KI and McAlister-Henn L (1991) Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase. Mol Cell Biol 11(1):370-80
2)	Lorenz MC and Fink GR (2001) The glyoxylate cycle is required for fungal virulence. Nature 412(6842):83-6
3)	Gibson N and McAlister-Henn L (2003) Physical and genetic interactions of cytosolic malate dehydrogenase with other gluconeogenic enzymes. J Biol Chem 278(28):25628-36
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41