PAP2/YOL115W Protein Information Help

Standard Name Pap2p 1
Systematic Name Yol115wp
Alias Trf4p 2
ORF Classification Verified
Description Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p; PAP2 has a paralog, TRF5, that arose from the whole genome duplication (1, 3, 4, 5, 6)
Name Description Poly(A) Polymerase 1, 2, 7
Experimental Data
Molecules/cell 7550 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 584
Molecular Weight (Da) 66,030
Isoelectric Point (pI) 5.26

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pap2p (InterPro)
Physical Interactions There are 70 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000005309C | P53632
MIPS: YOL115W
NCBI: 1419987 | 1717744 | 302148910 | 51830518 | 6324457 | 663237 | 950226 | NP_014526.1
GenBank/EMBL/DDBJ: DAA10668.1 | AY723865 | U31355 | Z48149 | Z74857
External Classifications EC: 2.7.7.- [Transferases transferring phosphorous-containing groups nucleotidyltransferases]
Amino Acid Sequence (or in FASTA format)
 
       1  MGAKSVTASS SKKIKNRHNG KVKKSKKIKK VRKPQKSISL NDENEVEILP
      51  SRNEQETNKL PKDHVTADGI LVLEHKSDDD EGFDVYDGHF DNPTDIPSTT
     101  EESKTPSLAV HGDEKDLANN DDFISLSASS EDEQAEQEEE REKQELEIKK
     151  EKQKEILNTD YPWILNHDHS KQKEISDWLT FEIKDFVAYI SPSREEIEIR
     201  NQTISTIREA VKQLWPDADL HVFGSYSTDL YLPGSDIDCV VTSELGGKES
     251  RNNLYSLASH LKKKNLATEV EVVAKARVPI IKFVEPHSGI HIDVSFERTN
     301  GIEAAKLIRE WLDDTPGLRE LVLIVKQFLH ARRLNNVHTG GLGGFSIICL
     351  VFSFLHMHPR IITNEIDPKD NLGVLLIEFF ELYGKNFGYD DVALGSSDGY
     401  PVYFPKSTWS AIQPIKNPFS LAIQDPGDES NNISRGSFNI RDIKKAFAGA
     451  FDLLTNRCFE LHSATFKDRL GKSILGNVIK YRGKARDFKD ERGLVLNKAI
     501  IENENYHKKR SRIIHDEDFA EDTVTSTATA TTTDDDYEIT NPPAKKAKIE
     551  EKPESEPAKR NSGETYITVS SEDDDEDGYN PYTL*

external links for Pap2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Pap2p
1) Vanacova S, et al.  (2005) A new yeast poly(A) polymerase complex involved in RNA quality control. PLoS Biol 3(6):e189
2) Sadoff BU, et al.  (1995) Isolation of mutants of Saccharomyces cerevisiae requiring DNA topoisomerase I. Genetics 141(2):465-79
3) LaCava J, et al.  (2005) RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. Cell 121(5):713-24
4) Wyers F, et al.  (2005) Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase. Cell 121(5):725-37
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Gellon L, et al.  (2008) Intrinsic 5'-deoxyribose-5-phosphate lyase activity in Saccharomyces cerevisiae Trf4 protein with a possible role in base excision DNA repair. DNA Repair (Amst) 7(2):187-98
7) Castano IB, et al.  (1996) A novel family of TRF (DNA topoisomerase I-related function) genes required for proper nuclear segregation. Nucleic Acids Res 24(12):2404-10
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41