SKM1/YOL113W Protein Information Help

Standard Name Skm1p 1
Systematic Name Yol113wp
ORF Classification Verified
Description Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description STE20/PAK homologous Kinase related to Morphogenesis 1
Experimental Data
Molecules/cell 238 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 655
Molecular Weight (Da) 75,331
Isoelectric Point (pI) 9.25

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Skm1p (InterPro)
Physical Interactions There are 65 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001359E0 | Q12469
MIPS: YOL113W
NCBI: 1129169 | 1419983 | 2499622 | 51013889 | 6324459 | 663239 | NP_014528.1
GenBank/EMBL/DDBJ: DAA10670.1 | AY693219 | X69322 | Z48149 | Z74855
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MKGVKKEGWI SYKVDGLFSF LWQKRYLVLN DSYLAFYKSD KCNEEPVLSV
      51  PLTSITNVSR IQLKQNCFEI LRATDQKENI SPINSYFYES NSKRSIFIST
     101  RTERDLHGWL DAIFAKCPLL SGVSSPTNFT HKVHVGFDPK VGNFVGVPDS
     151  WAKLLQTSEI TYDDWNRNSK AVIKALQFYE DYNGLDTMQF NDHLNTSLDL
     201  KPLKSPTRYI INKRTNSIKR SVSRTLRKGK TDSILPVYQS ELKPFPRPSD
     251  DDYKFTNIED NKVREEGRVH VSKESTADSQ TKQLGKKEQK VIQSHLRRHD
     301  NNSTFRPHRL APSAPATKNH DSKTKWHKED LLELKNNDDS NEIIMKMKTV
     351  AIDVNPRPYF QLVEKAGQGA SGAVYLSKRI KLPQENDPRF LKSHCHRVVG
     401  ERVAIKQIRL SEQPKKQLIM NELLVMNDSR QENIVNFLEA YIIDDEELWV
     451  IMEYMEGGCL TDILDAVARS NTGEHSSPLN ENQMAYIVKE TCQGLKFLHN
     501  KKIIHRDIKS DNILLNSQGL VKITDFGFCV ELTEKRSKRA TMVGTPYWMA
     551  PEIVNQKGYD EKVDVWSLGI MLIEMIEGEP PYLNEDPLKA LYLIANNGSP
     601  KLRHPESVSK QTKQFLDACL QVNVESRASV RKLLTFEFLS MACSPEQLKV
     651  SLKWH*

external links for Skm1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Skm1p
1) Martin H, et al.  (1997) Characterization of SKM1, a Saccharomyces cerevisiae gene encoding a novel Ste20/PAK-like protein kinase. Mol Microbiol 23(3):431-44
2) Richman TJ, et al.  (1999) The Cdc42p GTPase is involved in a G2/M morphogenetic checkpoint regulating the apical-isotropic switch and nuclear division in yeast. J Biol Chem 274(24):16861-70
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Lin M, et al.  (2009) The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway. Mol Biol Cell 20(22):4826-37
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41