PKH2/YOL100W Protein Information

Standard Name	Pkh2p 1
Systematic Name	Yol100wp
ORF Classification	Verified
Description	Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Pkb-activating Kinase Homolog 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,081
Molecular Weight (Da)	121,659
Isoelectric Point (pI)	10.46

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pkh2p (InterPro)
Physical Interactions	There are 61 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004F9FC \| Q12236 MIPS: YOL100W NCBI: 1419952 \| 2499623 \| 6324472 \| 663254 \| NP_014541.1 GenBank/EMBL/DDBJ: DAA10684.1 \| Z48149 \| Z74842
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MYFDKDNSMS PRPLLPSDEQ KLNINLLTKK EKFSHLDPHY DAKATPQRST 51 SNRNVGDLLL EKRTAKPMIQ KALTNTDNFI EMYHNQQRKN LDDDTIKEVM 101 INDENGKTVA STNDGRYDND YDNNDINDQK TLDNIAGSPH MEKNRNKVKI 151 EHDSSSQKPI AKESSKAQKN IIKKGIKDFK FGSVIGDGAY STVMLATSID 201 TKKRYAAKVL NKEYLIRQKK VKYVSIEKTA LQKLNNSPSV VRLFSTFQDE 251 SSLYFLLEYA PNGDFLSLMK KYGSLDETCA RYYAAQIIDA IDYLHSNGII 301 HRDIKPENIL LDGEMKIKLT DFGTAKLLNP TNNSVSKPEY DLSTRSKSFV 351 GTAEYVSPEL LNDSFTDYRC DIWAFGCILF QMIAGKPPFK ATNEYLTFQK 401 VMKVQYAFTP GFPLIIRDLV KKILVKNLDR RLTISQIKEH HFFKDLNFKD 451 GSVWSKTPPE IKPYKINAKS MQAMPSGSDR KLVKKSVNTL GKSHLVTQRS 501 ASSPSVEETT HSTLYNNNTH ASTESEISIK KRPTDERTAQ ILENARKGIN 551 NRKNQPGKRT PSGAASAALA ASAALTKKTM QSYPTSSSKS SRSSSPATTS 601 RPGTYKRTSS TESKPFAKSP PLSASVLSSK VPMPPYTPPM SPPMTPYDTY 651 QMTPPYTTKQ QDYSDTAIAA PKPCISKQNV KNSTDSPLMN KQDIQWSFYL 701 KNINEHVLRT EKLDFVTTNY DILEKKMLKL NGSLLDPQLF GKPRHTFLSQ 751 VARSGGEVTG FRNDPTMTAY SKTEDTYYSK NIIDLQLLED DYRIEGGDLS 801 ELLTNRSGEG YKCNQNSSPM KDDDKSESNN KGSSVFSGKI KKLFHPTSAA 851 ETLSSSDEKT KYYKRTIVMT SFGRFLVFAK RRQPNPVTNL KYELEYDINL 901 RQQGTKIKEL IIPLEMGTNH IVVIQTPYKS FLLSTDKKTT SKLFTVLKKI 951 LNSNTNKIEK ELLQRNQKVI ERRTSSSGRA IPKDLPTSKS PSPKPRTHSQ 1001 SPSISKHNSF SESINSAKSN RSSRIFETFI NAKEQNSKKH AAPVPLTSKL 1051 VNGLPKRQVT VGLGLNTGTN FKNSSAKSKR S*

Amino Acid Sequence (or in FASTA format)

       1  MYFDKDNSMS PRPLLPSDEQ KLNINLLTKK EKFSHLDPHY DAKATPQRST
      51  SNRNVGDLLL EKRTAKPMIQ KALTNTDNFI EMYHNQQRKN LDDDTIKEVM
     101  INDENGKTVA STNDGRYDND YDNNDINDQK TLDNIAGSPH MEKNRNKVKI
     151  EHDSSSQKPI AKESSKAQKN IIKKGIKDFK FGSVIGDGAY STVMLATSID
     201  TKKRYAAKVL NKEYLIRQKK VKYVSIEKTA LQKLNNSPSV VRLFSTFQDE
     251  SSLYFLLEYA PNGDFLSLMK KYGSLDETCA RYYAAQIIDA IDYLHSNGII
     301  HRDIKPENIL LDGEMKIKLT DFGTAKLLNP TNNSVSKPEY DLSTRSKSFV
     351  GTAEYVSPEL LNDSFTDYRC DIWAFGCILF QMIAGKPPFK ATNEYLTFQK
     401  VMKVQYAFTP GFPLIIRDLV KKILVKNLDR RLTISQIKEH HFFKDLNFKD
     451  GSVWSKTPPE IKPYKINAKS MQAMPSGSDR KLVKKSVNTL GKSHLVTQRS
     501  ASSPSVEETT HSTLYNNNTH ASTESEISIK KRPTDERTAQ ILENARKGIN
     551  NRKNQPGKRT PSGAASAALA ASAALTKKTM QSYPTSSSKS SRSSSPATTS
     601  RPGTYKRTSS TESKPFAKSP PLSASVLSSK VPMPPYTPPM SPPMTPYDTY
     651  QMTPPYTTKQ QDYSDTAIAA PKPCISKQNV KNSTDSPLMN KQDIQWSFYL
     701  KNINEHVLRT EKLDFVTTNY DILEKKMLKL NGSLLDPQLF GKPRHTFLSQ
     751  VARSGGEVTG FRNDPTMTAY SKTEDTYYSK NIIDLQLLED DYRIEGGDLS
     801  ELLTNRSGEG YKCNQNSSPM KDDDKSESNN KGSSVFSGKI KKLFHPTSAA
     851  ETLSSSDEKT KYYKRTIVMT SFGRFLVFAK RRQPNPVTNL KYELEYDINL
     901  RQQGTKIKEL IIPLEMGTNH IVVIQTPYKS FLLSTDKKTT SKLFTVLKKI
     951  LNSNTNKIEK ELLQRNQKVI ERRTSSSGRA IPKDLPTSKS PSPKPRTHSQ
    1001  SPSISKHNSF SESINSAKSN RSSRIFETFI NAKEQNSKKH AAPVPLTSKL
    1051  VNGLPKRQVT VGLGLNTGTN FKNSSAKSKR S*

external links for Pkh2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Pkh2p

1)	Casamayor A, et al. (1999) Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Curr Biol 9(4):186-97
2)	Inagaki M, et al. (1999) PDK1 homologs activate the Pkc1-mitogen-activated protein kinase pathway in yeast. Mol Cell Biol 19(12):8344-52
3)	Friant S, et al. (2001) Sphingoid base signaling via Pkh kinases is required for endocytosis in yeast. EMBO J 20(23):6783-92
4)	Roelants FM, et al. (2002) Pkh1 and Pkh2 differentially phosphorylate and activate Ypk1 and Ykr2 and define protein kinase modules required for maintenance of cell wall integrity. Mol Biol Cell 13(9):3005-28
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61