HMI1/YOL095C Protein Information

Standard Name	Hmi1p 1
Systematic Name	Yol095cp
ORF Classification	Verified
Description	Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD (2, 3)
Name Description	Helicase in MItochondria 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	706
Molecular Weight (Da)	80,575
Isoelectric Point (pI)	8.93

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hmi1p (InterPro)
Physical Interactions	There are 4 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000698BB \| Q12039 MIPS: YOL095C NCBI: 1419944 \| 59799592 \| 600463 \| 6324477 \| 663258 \| 841469 \| NP_014546.1 GenBank/EMBL/DDBJ: DAA10689.1 \| U26030 \| X83121 \| Z48149 \| Z74837
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.12 [DNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MDKLTPSQWK VINKSYEPAS TIKVIAGPGS GKTLTLLYKV LHLITVENIK 51 PEEILIFSLT NKAVDSIIEN LLSIFENSHT NKEIVHQIGC YTVHGLANRI 101 VVENEGMINI IEEIGWRGLM KLLPPSKRTP HHFRSYKELE KVVKDYKLNN 151 AKNNNPVIEK LVELMDNCKV MTNDDLIIRA KKYLELDSSD SDASSFTQDL 201 RNKYKVVLID EFQDLYPSLA PLITMICKGK QLIMFGDTNQ SIYGFLGSNN 251 EIMSQLDNLH PKNSTTVLKL FDNFRSTPEI ISLASKIINR PLAEKQIIDD 301 TDETPSELVR KLPSGVSPQI MTFDDLAAES EFIIDKITQL ICSSAKFSDI 351 AILSRTNSHL TAIASILKKY GIPYQKLKSQ PDWMDDLRIQ FLLDILKVCS 401 LASDEKHNRE FNTGDKWQSN FSILVTMSAL KGIGDASIQA LYKACSLKNL 451 SIWKYLTMVP NFEWPLGLSI KKKMENYTSN LYEMIENDQV HQLDDPMELL 501 EKVASITNNL NLNPTYFQSL SDAQSSLEFK THLQEMAQVM KVSKSNKPPG 551 ISFVKWFLET YFDQTMVFHQ SQQALQTTGP GTVKLSTIHS AKGLEFPIVF 601 LTNGSMSNFP MDTNALYVGI TRARNLLYMC NMKHERLVSK SSPYSRNIMS 651 NNLFWTYYNK DLKRSVCDVK VTHGYNVQRY NQLRKNFGFY RAYSSLRGCK 701 SVFRRI*

Amino Acid Sequence (or in FASTA format)

       1  MDKLTPSQWK VINKSYEPAS TIKVIAGPGS GKTLTLLYKV LHLITVENIK
      51  PEEILIFSLT NKAVDSIIEN LLSIFENSHT NKEIVHQIGC YTVHGLANRI
     101  VVENEGMINI IEEIGWRGLM KLLPPSKRTP HHFRSYKELE KVVKDYKLNN
     151  AKNNNPVIEK LVELMDNCKV MTNDDLIIRA KKYLELDSSD SDASSFTQDL
     201  RNKYKVVLID EFQDLYPSLA PLITMICKGK QLIMFGDTNQ SIYGFLGSNN
     251  EIMSQLDNLH PKNSTTVLKL FDNFRSTPEI ISLASKIINR PLAEKQIIDD
     301  TDETPSELVR KLPSGVSPQI MTFDDLAAES EFIIDKITQL ICSSAKFSDI
     351  AILSRTNSHL TAIASILKKY GIPYQKLKSQ PDWMDDLRIQ FLLDILKVCS
     401  LASDEKHNRE FNTGDKWQSN FSILVTMSAL KGIGDASIQA LYKACSLKNL
     451  SIWKYLTMVP NFEWPLGLSI KKKMENYTSN LYEMIENDQV HQLDDPMELL
     501  EKVASITNNL NLNPTYFQSL SDAQSSLEFK THLQEMAQVM KVSKSNKPPG
     551  ISFVKWFLET YFDQTMVFHQ SQQALQTTGP GTVKLSTIHS AKGLEFPIVF
     601  LTNGSMSNFP MDTNALYVGI TRARNLLYMC NMKHERLVSK SSPYSRNIMS
     651  NNLFWTYYNK DLKRSVCDVK VTHGYNVQRY NQLRKNFGFY RAYSSLRGCK
     701  SVFRRI*

external links for Hmi1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Hmi1p

1)	Lecrenier, N. and F. Foury (1998) personal communication
2)	Sedman T, et al. (2000) A DNA helicase required for maintenance of the functional mitochondrial genome in Saccharomyces cerevisiae. Mol Cell Biol 20(5):1816-24
3)	Lee CM, et al. (1999) The DNA helicase, Hmi1p, is transported into mitochondria by a C-terminal cleavable targeting signal. J Biol Chem 274(30):20937-42