DUF1/YOL087C Protein Information Help

Standard Name Duf1p 1, 2
Systematic Name Yol087cp
ORF Classification Verified
Description Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid (2, 3, 4)
Name Description DUB-associated Factor 1 1
Experimental Data
Molecules/cell 589 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,116
Molecular Weight (Da) 125,381
Isoelectric Point (pI) 6.67

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Duf1p (InterPro)
Physical Interactions There are 28 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006ABF0 | Q99247
MIPS: YOL087C
NCBI: 1419928 | 600471 | 6324485 | 74698026 | NP_014554.1
GenBank/EMBL/DDBJ: DAA10697.1 | X83121 | Z74829
Amino Acid Sequence (or in FASTA format)
 
       1  MNQLTVSYGL ISPDYCTSQD AHILPITKIL YPDIPGKNYF LTSGRDGSII
      51  LHKNTQLSNE PETAATTIKN DAIRMQVHSD WASDLIHVNM KNSDPSAGDT
     101  FISVSHDFSI VLISVNAQLT TWDKKIIGDH DDYIKCIVPI HYEMSNDYEL
     151  EEQEGGPDNV HDGINNGIVV DEQNNFLFVT GGLDRKIKLW CLSSGPEKMA
     201  TLLHTFDNAQ SNDTGSIYSM SPIIPKYSFD DNQTSRPFDF VAGDCNGDLI
     251  FYSCKYRKEV IRIQNAHRTN IKVVRTLDDS TRLISTSSDG VINVWDLNCR
     301  HDQTTGALQL PKKIGSWSWD SSIWCVQGTS LDKLYFGDSQ GNVMRANLSS
     351  YEDAKLTRIF KPDHHHHHHH HHEHEEQNIS TTDAKVKKYG GILDIALLPN
     401  EKLLFSFCTD SNLNVLDLTN NHFSVNEGGF ALTRSSLLTN RRHVITENTK
     451  GQMQRWDIVS CELLNTFDSS EGSFDDIVMK YTSKEILSHW CTVSVKVGML
     501  FVKINPKFLK TEVYGSALKD YQVVNNIEIN SDERYNLGKI VINSLFNEFI
     551  SYEVQKDKLL RKKIFSLKKK DLTNSLTLDT GYNSESKKNN KDKKRKSTFK
     601  ISSTLSIGNT NSSGTPPNSA PATPVMAETI VLEEQPLLQS ASDKAIDDSL
     651  ELVQPLPASK KPYFRTQSSG SLLSRKFKSF RSTSGRATTG LNTPEEPKGI
     701  LPDTPHVIND DSAFPQAINT TQQSKDATPE SMLWNHPFKL EQKLSAISSQ
     751  DLPSNNTHNK LRSSENSRAN STSTLEGNEK KKPEFMPDLL EQIQESYKQQ
     801  YMNTSSLKYL TKRLPVTKII KASSCPIIRV KSATLVLVHL WKEGSCGGRV
     851  LFSTLLPPSH VDNETVSGGK ENSKPPDDEE VDLQAVDDDK LGKYDLIDGE
     901  LGSRLNRRQI FEQLEENLPY WFAKALFRDI KTVEEQPKLN FLIMPWSSVG
     951  GSEAAGNENK KKFISASDTT ESSGNDSSDS SLGNGNEAVS PSTQQQFHNM
    1001  LKFGRPKTSE QELNPTDLPR ISEANVKLVA PGMIRVKKIK LYVADRFETK
    1051  TPEMKAKMEP SLWLDLLCRG QVLDNDMTLN TVRTLYWKSQ GDIVLEYRRK
    1101  VHNSPLVHEV NGNEGK*

external links for Duf1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Duf1p
1) Bernard D, et al.  (2008) personal communication
2) Pashkova N, et al.  (2010) WD40 repeat propellers define a ubiquitin-binding domain that regulates turnover of F box proteins. Mol Cell 40(3):433-43
3) Rieger KJ, et al.  (1999) Chemotyping of yeast mutants using robotics. Yeast 15(10B):973-86
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41