INP54/YOL065C Protein Information

Standard Name	Inp54p 1
Systematic Name	Yol065cp
ORF Classification	Verified
Description	Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins (2)
Name Description	INositol polyphosphate 5-Phosphatase 3

Experimental Data
Molecules/cell	1200 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	384
Molecular Weight (Da)	43,799
Isoelectric Point (pI)	7.6

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Inp54p (InterPro)
Physical Interactions	There are 7 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006C643 \| Q08227 MIPS: YOL065C NCBI: 1419885 \| 6324507 \| 74627240 \| NP_014576.1 GenBank/EMBL/DDBJ: DAA10718.1 \| Z74807
External Classifications	EC: 3.1.3.36 [Phosphoinositide 5-phosphatase]

Amino Acid Sequence (or in FASTA format)
1 MNKTNWKVSV TTFNCGKEFP VENSKAIVKQ LLFPYDDGIS QLELQDLYVL 51 GFQEVVPIWQ GSFPAVNRDL IDRITTTAVN CLNEKVSATQ GDEQYSCLGV 101 NSLGAITIIV LYNNNALKVK DDILKRNGKC GWFGTHLKGG TLISFQMTRN 151 GEENWERFSY ICAHLNANEG VNNRNQRIDD YKRIMSEVCD SEVAKSDHFF 201 FLGDLNFRVT STYDPTTNYS STTTLRRLLE NHEELNLLRK GEDEPLCKGF 251 QELKITFPPT YKFKLFEKET YNTKRIPSWC DRILYKSYAV PTFAQEGTYH 301 SVPRSNALLF SDHQPVNLTV RLPRSTGTPV PLSLHIEKYP LSWSSGLIGQ 351 IGDAVIGYCG WLVTKNVHYW ILGSLLLYLL LKIL*

Amino Acid Sequence (or in FASTA format)

       1  MNKTNWKVSV TTFNCGKEFP VENSKAIVKQ LLFPYDDGIS QLELQDLYVL
      51  GFQEVVPIWQ GSFPAVNRDL IDRITTTAVN CLNEKVSATQ GDEQYSCLGV
     101  NSLGAITIIV LYNNNALKVK DDILKRNGKC GWFGTHLKGG TLISFQMTRN
     151  GEENWERFSY ICAHLNANEG VNNRNQRIDD YKRIMSEVCD SEVAKSDHFF
     201  FLGDLNFRVT STYDPTTNYS STTTLRRLLE NHEELNLLRK GEDEPLCKGF
     251  QELKITFPPT YKFKLFEKET YNTKRIPSWC DRILYKSYAV PTFAQEGTYH
     301  SVPRSNALLF SDHQPVNLTV RLPRSTGTPV PLSLHIEKYP LSWSSGLIGQ
     351  IGDAVIGYCG WLVTKNVHYW ILGSLLLYLL LKIL*

external links for Inp54p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Inp54p

1)	Stolz LE, et al. (1998) Identification and characterization of an essential family of inositol polyphosphate 5-phosphatases (INP51, INP52 and INP53 gene products) in the yeast Saccharomyces cerevisiae. Genetics 148(4):1715-29
2)	Wiradjaja F, et al. (2001) The yeast inositol polyphosphate 5-phosphatase Inp54p localizes to the endoplasmic reticulum via a C-terminal hydrophobic anchoring tail: regulation of secretion from the endoplasmic reticulum. J Biol Chem 276(10):7643-53
3)	Stolz LE, et al. (1998) INP51, a yeast inositol polyphosphate 5-phosphatase required for phosphatidylinositol 4,5-bisphosphate homeostasis and whose absence confers a cold-resistant phenotype. J Biol Chem 273(19):11852-61
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41