GPD2/YOL059W Protein Information Help

Standard Name Gpd2p
Systematic Name Yol059wp
Alias Gpd3p
ORF Classification Verified
Description NAD-dependent glycerol 3-phosphate dehydrogenase; expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1; GPD2 has a paralog, GPD1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Glycerol-3-Phosphate Dehydrogenase 1
Experimental Data
Molecules/cell 8970 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 440
Molecular Weight (Da) 49,421
Isoelectric Point (pI) 7.08

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Gpd2p (InterPro)
Physical Interactions There are 21 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012B96E | P41911
MIPS: YOL059W
NCBI: 1419873 | 1708024 | 45271010 | 511135 | 6324513 | 984182 | NP_014582.1
GenBank/EMBL/DDBJ: DAA10724.1 | AY558560 | X91067 | Z35169 | Z74801
External Classifications EC: 1.1.1.8 [Glycerol-3-phosphate dehydrogenase (NAD(+))]
Amino Acid Sequence (or in FASTA format)
 
       1  MLAVRRLTRY TFLKRTHPVL YTRRAYKILP SRSTFLRRSL LQTQLHSKMT
      51  AHTNIKQHKH CHEDHPIRRS DSAVSIVHLK RAPFKVTVIG SGNWGTTIAK
     101  VIAENTELHS HIFEPEVRMW VFDEKIGDEN LTDIINTRHQ NVKYLPNIDL
     151  PHNLVADPDL LHSIKGADIL VFNIPHQFLP NIVKQLQGHV APHVRAISCL
     201  KGFELGSKGV QLLSSYVTDE LGIQCGALSG ANLAPEVAKE HWSETTVAYQ
     251  LPKDYQGDGK DVDHKILKLL FHRPYFHVNV IDDVAGISIA GALKNVVALA
     301  CGFVEGMGWG NNASAAIQRL GLGEIIKFGR MFFPESKVET YYQESAGVAD
     351  LITTCSGGRN VKVATYMAKT GKSALEAEKE LLNGQSAQGI ITCREVHEWL
     401  QTCELTQEFP LFEAVYQIVY NNVRMEDLPE MIEELDIDDE *

external links for Gpd2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains YeastRC Public Image Repository

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Gpd2p
1) Eriksson P, et al.  (1995) Cloning and characterization of GPD2, a second gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) in Saccharomyces cerevisiae, and its comparison with GPD1. Mol Microbiol 17(1):95-107
2) Ansell R, et al.  (1997) The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation. EMBO J 16(9):2179-87
3) Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Lee YJ, et al.  (2012) Reciprocal phosphorylation of yeast glycerol-3-phosphate dehydrogenases in adaptation to distinct types of stress. Mol Cell Biol 32(22):4705-17
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41