PSH1/YOL054W Protein Information Help

Standard Name Psh1p 1
Systematic Name Yol054wp
ORF Classification Verified
Description E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates poyubiquitination and degradation of Cse4p; prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p (2, 3)
Name Description Pob3/Spt16 Histone associated
Experimental Data
Molecules/cell 6490 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 406
Molecular Weight (Da) 47,068
Isoelectric Point (pI) 4.09

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Psh1p (InterPro)
Physical Interactions There are 89 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000530C3 | Q12161
MIPS: YOL054W
NCBI: 1419863 | 6324518 | 74676541 | 984189 | NP_014587.1 | NM_001183309.1
GenBank/EMBL/DDBJ: CAA62533.1 | CAA99062.1 | DAA10729.1 | X91067 | Z74796
Amino Acid Sequence (or in FASTA format)
       1  MGDELHNRLL HQNDGTKDAI LYKIIESLVC SICHDYMFVP MMTPCGHNYC
      51  YGCLNTWFAS NTQKELACPQ CRSDITTIPA LNTTLQQYLS FILEKLRDQN
     101  DESFKKLLTT KTKEENDYKN DKEKDTLFDK VFKNSALAVA DDSDDGITRC
     151  SNCHWELDPD EVEDGNVCPH CNARIRNYAG GRDEFDEEEY SEGELDEIRE
     201  SMRRRRENRF ASTNPFANRD DVSSEDDDSS EEEPMREHIP LGRWARSHNR
     251  SIAVDAVDDE DDEEEDEEEE EEMDSDLKDF IEDDEDDEDE DGSRRNLVLS
     301  ALKNRHVIIT DDEEEEQRRH ATEEEDRDSD FYEHNDDGFV SGDSLDEDQK
     351  EVTRIQSSSD SEDRSLSYSG SSDVKDNNDD NTEELDDPQP KRQKRFRVVL
     401  GDSDDE*                                               

external links for Psh1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) CYC2008 (complexes) MIPS YeastGFP
YGOB Complexome Pfam domains YeastRC Public Image Repository
YOGY DIP YeastRC Structure Prediction (Seattle)

GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Psh1p
1) Li AM, et al.  (2003) Scp160p associates with specific mRNAs in yeast. Nucleic Acids Res 31(7):1830-7
2) Ranjitkar P, et al.  (2010) An E3 Ubiquitin Ligase Prevents Ectopic Localization of the Centromeric Histone H3 Variant via the Centromere Targeting Domain. Mol Cell 40(3):455-64
3) Hewawasam G, et al.  (2010) Psh1 Is an E3 Ubiquitin Ligase that Targets the Centromeric Histone Variant Cse4. Mol Cell 40(3):444-54
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41