GAL11/YOL051W Protein Information

Standard Name	Gal11p
Systematic Name	Yol051wp
Alias	Rar3p , Sds4p 1 , Spt13p , Abe1p , Med15p 2
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator (3, 4, 5, 6, 7)
Name Description	GALactose metabolism

Experimental Data
Molecules/cell	606 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,081
Molecular Weight (Da)	120,308
Isoelectric Point (pI)	10.61

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gal11p (InterPro)
Physical Interactions	There are 213 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005314F \| P19659 MIPS: YOL051W NCBI: 1419856 \| 170784969 \| 171550 \| 2507028 \| 377656187 \| 6324522 \| 984193 \| NP_014591.1 GenBank/EMBL/DDBJ: DAA10733.1 \| M22481 \| X91067 \| Z74793

Amino Acid Sequence (or in FASTA format)
1 MSAAPVQDKD TLSNAERAKN VNGLLQVLMD INTLNGGSSD TADKIRIHAK 51 NFEAALFAKS SSKKEYMDSM NEKVAVMRNT YNTRKNAVTA AAANNNIKPV 101 EQHHINNLKN SGNSANNMNV NMNLNPQMFL NQQAQARQQV AQQLRNQQQQ 151 QQQQQQQQRR QLTPQQQQLV NQMKVAPIPK QLLQRIPNIP PNINTWQQVT 201 ALAQQKLLTP QDMEAAKEVY KIHQQLLFKA RLQQQQAQAQ AQANNNNNGL 251 PQNGNINNNI NIPQQQQMQP PNSSANNNPL QQQSSQNTVP NVLNQINQIF 301 SPEEQRSLLQ EAIETCKNFE KTQLGSTMTE PVKQSFIRKY INQKALRKIQ 351 ALRDVKNNNN ANNNGSNLQR AQNVPMNIIQ QQQQQNTNNN DTIATSATPN 401 AAAFSQQQNA SSKLYQMQQQ QQAQAQAQAQ AQAQAQAQAQ AQAAQAAQAQ 451 AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AHAQHQPSQQ PQQAQQQPNP 501 LHGLTPTAKD VEVIKQLSLD ASKTNLRLTD VTNSLSNEEK EKIKMKLKQG 551 QKLFVQVSNF APQVYIITKN ENFLKEVFQL RIFVKEILEK CAEGIFVVKL 601 DTVDRLIIKY QKYWESMRIQ ILRRQAILRQ QQQMANNNGN PGTTSTGNNN 651 NIATQQNMQQ SLQQMQHLQQ LKMQQQQQQQ QQQQQQQQQQ QQQQQQHIYP 701 SSTPGVANYS AMANAPGNNI PYMNHKNTSS MDFLNSMENT PKVPVSAAAT 751 PSLNKTINGK VNGRTKSNTI PVTSIPSTNK KLSISNAASQ QPTPRSASNT 801 AKSTPNTNPS PLKTQTKNGT PNPNNMKTVQ SPMGAQPSYN SAIIENAFRK 851 EELLLKDLEI RKLEISSRFK HRQEIFKDSP MDLFMSTLGD CLGIKDEEML 901 TSCTIPKAVV DHINGSGKRK PTKAAQRARD QDSIDISIKD NKLVMKSKFN 951 KSNRSYSIAL SNVAAIFKGI GGNFKDLSTL VHSSSPSTSS NMDVGNPRKR 1001 KASVLEISPQ DSIASVLSPD SNIMSDSKKI KVDSPDDPFM TKSGATTSEK 1051 QEVTNEAPFL TSGTSSEQFN VWDWNNWTSA T*

Amino Acid Sequence (or in FASTA format)

       1  MSAAPVQDKD TLSNAERAKN VNGLLQVLMD INTLNGGSSD TADKIRIHAK
      51  NFEAALFAKS SSKKEYMDSM NEKVAVMRNT YNTRKNAVTA AAANNNIKPV
     101  EQHHINNLKN SGNSANNMNV NMNLNPQMFL NQQAQARQQV AQQLRNQQQQ
     151  QQQQQQQQRR QLTPQQQQLV NQMKVAPIPK QLLQRIPNIP PNINTWQQVT
     201  ALAQQKLLTP QDMEAAKEVY KIHQQLLFKA RLQQQQAQAQ AQANNNNNGL
     251  PQNGNINNNI NIPQQQQMQP PNSSANNNPL QQQSSQNTVP NVLNQINQIF
     301  SPEEQRSLLQ EAIETCKNFE KTQLGSTMTE PVKQSFIRKY INQKALRKIQ
     351  ALRDVKNNNN ANNNGSNLQR AQNVPMNIIQ QQQQQNTNNN DTIATSATPN
     401  AAAFSQQQNA SSKLYQMQQQ QQAQAQAQAQ AQAQAQAQAQ AQAAQAAQAQ
     451  AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AHAQHQPSQQ PQQAQQQPNP
     501  LHGLTPTAKD VEVIKQLSLD ASKTNLRLTD VTNSLSNEEK EKIKMKLKQG
     551  QKLFVQVSNF APQVYIITKN ENFLKEVFQL RIFVKEILEK CAEGIFVVKL
     601  DTVDRLIIKY QKYWESMRIQ ILRRQAILRQ QQQMANNNGN PGTTSTGNNN
     651  NIATQQNMQQ SLQQMQHLQQ LKMQQQQQQQ QQQQQQQQQQ QQQQQQHIYP
     701  SSTPGVANYS AMANAPGNNI PYMNHKNTSS MDFLNSMENT PKVPVSAAAT
     751  PSLNKTINGK VNGRTKSNTI PVTSIPSTNK KLSISNAASQ QPTPRSASNT
     801  AKSTPNTNPS PLKTQTKNGT PNPNNMKTVQ SPMGAQPSYN SAIIENAFRK
     851  EELLLKDLEI RKLEISSRFK HRQEIFKDSP MDLFMSTLGD CLGIKDEEML
     901  TSCTIPKAVV DHINGSGKRK PTKAAQRARD QDSIDISIKD NKLVMKSKFN
     951  KSNRSYSIAL SNVAAIFKGI GGNFKDLSTL VHSSSPSTSS NMDVGNPRKR
    1001  KASVLEISPQ DSIASVLSPD SNIMSDSKKI KVDSPDDPFM TKSGATTSEK
    1051  QEVTNEAPFL TSGTSSEQFN VWDWNNWTSA T*

external links for Gal11p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Gal11p

1)	Sussel L, et al. (1993) Epigenetic switching of transcriptional states: cis- and trans-acting factors affecting establishment of silencing at the HMR locus in Saccharomyces cerevisiae. Mol Cell Biol 13(7):3919-28
2)	Bourbon HM, et al. (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3)	Li Y, et al. (1995) Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme. Proc Natl Acad Sci U S A 92(24):10864-8
4)	Han SJ, et al. (2001) Med9/Cse2 and Gal11 modules are required for transcriptional repression of distinct group of genes. J Biol Chem 276(40):37020-6
5)	Mizuno T and Harashima S (2003) Gal11 is a general activator of basal transcription, whose activity is regulated by the general repressor Sin4 in yeast. Mol Genet Genomics 269(1):68-77
6)	Myers LC and Kornberg RD (2000) Mediator of transcriptional regulation. Annu Rev Biochem 69():729-49
7)	Guglielmi B, et al. (2004) A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res 32(18):5379-91
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41