GSH2/YOL049W Protein Information

Standard Name	Gsh2p 1, 2
Systematic Name	Yol049wp
ORF Classification	Verified
Description	Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock (1, 2, 3)
Name Description	Glutathione 1, 2, 4

Experimental Data
Molecules/cell	3430 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	491
Molecular Weight (Da)	55,815
Isoelectric Point (pI)	5.41

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gsh2p (InterPro)
Physical Interactions	There are 2 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000113350 \| Q08220 MIPS: YOL049W NCBI: 1419854 \| 2198534 \| 2495096 \| 27573834 \| 27573835 \| 27573836 \| 27573837 \| 51830516 \| 6324524 \| NP_014593.1 GenBank/EMBL/DDBJ: DAA10734.1 \| AY723864 \| Y13804 \| Z74791
External Classifications	EC: 6.3.2.3 [Glutathione synthase]

Amino Acid Sequence (or in FASTA format)
1 MAHYPPSKDQ LNELIQEVNQ WAITNGLSMY PPKFEENPSN ASVSPVTIYP 51 TPIPRKCFDE AVQIQPVFNE LYARITQDMA QPDSYLHKTT EALALSDSEF 101 TGKLWSLYLA TLKSAQYKKQ NFRLGIFRSD YLIDKKKGTE QIKQVEFNTV 151 SVSFAGLSEK VDRLHSYLNR ANKYDPKGPI YNDQNMVISD SGYLLSKALA 201 KAVESYKSQQ SSSTTSDPIV AFIVQRNERN VFDQKVLELN LLEKFGTKSV 251 RLTFDDVNDK LFIDDKTGKL FIRDTEQEIA VVYYRTGYTT TDYTSEKDWE 301 ARLFLEKSFA IKAPDLLTQL SGSKKIQQLL TDEGVLGKYI SDAEKKSSLL 351 KTFVKIYPLD DTKLGREGKR LALSEPSKYV LKPQREGGGN NVYKENIPNF 401 LKGIEERHWD AYILMELIEP ELNENNIILR DNKSYNEPII SELGIYGCVL 451 FNDEQVLSNE FSGSLLRSKF NTSNEGGVAA GFGCLDSIIL Y*

Amino Acid Sequence (or in FASTA format)

       1  MAHYPPSKDQ LNELIQEVNQ WAITNGLSMY PPKFEENPSN ASVSPVTIYP
      51  TPIPRKCFDE AVQIQPVFNE LYARITQDMA QPDSYLHKTT EALALSDSEF
     101  TGKLWSLYLA TLKSAQYKKQ NFRLGIFRSD YLIDKKKGTE QIKQVEFNTV
     151  SVSFAGLSEK VDRLHSYLNR ANKYDPKGPI YNDQNMVISD SGYLLSKALA
     201  KAVESYKSQQ SSSTTSDPIV AFIVQRNERN VFDQKVLELN LLEKFGTKSV
     251  RLTFDDVNDK LFIDDKTGKL FIRDTEQEIA VVYYRTGYTT TDYTSEKDWE
     301  ARLFLEKSFA IKAPDLLTQL SGSKKIQQLL TDEGVLGKYI SDAEKKSSLL
     351  KTFVKIYPLD DTKLGREGKR LALSEPSKYV LKPQREGGGN NVYKENIPNF
     401  LKGIEERHWD AYILMELIEP ELNENNIILR DNKSYNEPII SELGIYGCVL
     451  FNDEQVLSNE FSGSLLRSKF NTSNEGGVAA GFGCLDSIIL Y*

external links for Gsh2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Gsh2p

1)	Grant CM, et al. (1997) Glutathione synthetase is dispensable for growth under both normal and oxidative stress conditions in the yeast Saccharomyces cerevisiae due to an accumulation of the dipeptide gamma-glutamylcysteine. Mol Biol Cell 8(9):1699-707
2)	Inoue Y, et al. (1998) Molecular identification of glutathione synthetase (GSH2) gene from Saccharomyces cerevisiae. Biochim Biophys Acta 1395(3):315-20
3)	Sugiyama K, et al. (2000) The Yap1p-dependent induction of glutathione synthesis in heat shock response of Saccharomyces cerevisiae. J Biol Chem 275(20):15535-40
4)	OhtakeY, et al. (1990) Isolation and characterization of glutathione biosynthesis-deficient mutants in Saccharomyces cerevisiae. Agric Biol Chem 54:3145-3150
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41