DIS3/YOL021C Protein Information 
| Standard Name | Dis3p 1 |
|---|---|
| Systematic Name | Yol021cp |
| Alias | Rrp44p 2 , Mtr17p 3 |
| ORF Classification | Verified |
| Description | Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress (10, 11, 4, 5, 6, 7, 8, 9) |
| Name Description | homolog of S. pombe dis3 (chromosome DISjunction) 1 |
| Experimental Data | |
|---|---|
| Molecules/cell | 606 12 |
| Click on image for expanded interactive view |
|---|
| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|---|
| Domains/motifs | See the graphical view and list of proteins that share domains/motifs in common with Dis3p (InterPro) |
| Physical Interactions | There are 176 total physical interactions (BioGRID) |
| Homologs | PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer |
| External Sequence Databases |
EBI: UPI0000052F2F | Q08162 MIPS: YOL021C NCBI: 1419801 | 1754617 | 178847337 | 2500547 | 266618486 | 448262642 | 6324552 | NP_014621.1 GenBank/EMBL/DDBJ: DAA10760.1 | D76430 | Z74763 |
| External Classifications | EC: 3.1.13.- [Exoribonucleases Producing 5'-Phosphomonoesters] EC: 3.1.26.- [Endoribonucleases Producing 5'-Phosphomonoesters] |
external links for Dis3p
| Homologs | Interaction Resources | Protein databases/Other | Localization Resources |
|---|---|---|---|
| BLASTP (NCBI) | BioGRID | SCOP Superfamily | Organelle DB |
| Ashbya (AGD) | BOND | GPMdb (Mass Spec.) | YPL+ |
| Aspergillus (AspGD) | BioPIXIE | MIPS | YeastGFP |
| Candida (CGD) | CYC2008 (complexes) | Pfam domains | YeastRC Public Image Repository |
| Candida (CandidaDB) | Complexome | YeastRC Structure Prediction (Seattle) | |
| YGOB | DIP | ||
| YOGY | GeneMANIA | ||
| YeastRC Two-Hybrid (Seattle) |
References cited on this page View Complete Literature Guide for Dis3p
| 1) | Noguchi E, et al. (1996) Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1. EMBO J 15(20):5595-605 |
| 2) | Mitchell P, et al. (1997) The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases. Cell 91(4):457-66 |
| 3) | Smith SB, et al. (2011) Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3 mutant. Yeast 28(11):755-69 |
| 4) | Shiomi T, et al. (1998) Human dis3p, which binds to either GTP- or GDP-Ran, complements Saccharomyces cerevisiae dis3. J Biochem (Tokyo) 123(5):883-90 |
| 5) | Liu Q, et al. (2006) Reconstitution, activities, and structure of the eukaryotic RNA exosome. Cell 127(6):1223-37 |
| 6) | Dziembowski A, et al. (2007) A single subunit, Dis3, is essentially responsible for yeast exosome core activity. Nat Struct Mol Biol 14(1):15-22 |
| 7) | Lorentzen E, et al. (2008) Structure of the active subunit of the yeast exosome core, Rrp44: diverse modes of substrate recruitment in the RNase II nuclease family. Mol Cell 29(6):717-28 |
| 8) | Lebreton A, et al. (2008) Endonucleolytic RNA cleavage by a eukaryotic exosome. Nature 456(7224):993-6 |
| 9) | Schneider C, et al. (2009) The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome. Nucleic Acids Res 37(4):1127-40 |
| 10) | Schaeffer D, et al. (2009) The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities. Nat Struct Mol Biol 16(1):56-62 |
| 11) | Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76 |
| 12) | Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41 |
