CMK2/YOL016C Protein Information

Standard Name	Cmk2p 1, 2
Systematic Name	Yol016cp
ORF Classification	Verified
Description	Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	CalModulin dependent protein Kinase 1, 2

Experimental Data
Molecules/cell	7500 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	447
Molecular Weight (Da)	50,447
Isoelectric Point (pI)	5.92

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cmk2p (InterPro)
Physical Interactions	There are 74 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012DC8E \| P22517 MIPS: YOL016C NCBI: 1419791 \| 218419 \| 2507190 \| 3556 \| 6324557 \| NP_014626.1 GenBank/EMBL/DDBJ: DAA10766.1 \| D90376 \| X56961 \| Z74758
External Classifications	EC: 2.7.11.17 [Calcium/calmodulin-dependent protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MPKESEVINS EFHVDVQDPE RLNGHPVAKF INKLSGQPES YVNRTNYIFG 51 RTLGAGSFGV VRQARKLSTN EDVAIKILLK KALQGNNVQL QMLYEELSIL 101 QKLSHPNIVS FKDWFESKDK FYIVTQLATG GELFDRILSR GKFTEVDAVE 151 IIVQILGAVE YMHSKNVVHR DLKPENVLYV DKSENSPLVI ADFGIAKQLK 201 GEEDLIYKAA GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSPFI 251 AESVEGFMEE CTASRYPVTF HMPYWDNISI DVKRFILKAL RLNPADRPTA 301 TELLDDPWIT SKRVETSNIL PDVKKGFSLR KKLRDAIEIV KLNNRIKRLR 351 NMYSLGDDGD NDIEENSLNE SLLDGVTHSL DDLRLQSQKK GGELTEEQMK 401 LKSALTKDAF VQIVKAATKN KHKVLAGEEE DDSKKTLHDD RESKSED*

Amino Acid Sequence (or in FASTA format)

       1  MPKESEVINS EFHVDVQDPE RLNGHPVAKF INKLSGQPES YVNRTNYIFG
      51  RTLGAGSFGV VRQARKLSTN EDVAIKILLK KALQGNNVQL QMLYEELSIL
     101  QKLSHPNIVS FKDWFESKDK FYIVTQLATG GELFDRILSR GKFTEVDAVE
     151  IIVQILGAVE YMHSKNVVHR DLKPENVLYV DKSENSPLVI ADFGIAKQLK
     201  GEEDLIYKAA GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSPFI
     251  AESVEGFMEE CTASRYPVTF HMPYWDNISI DVKRFILKAL RLNPADRPTA
     301  TELLDDPWIT SKRVETSNIL PDVKKGFSLR KKLRDAIEIV KLNNRIKRLR
     351  NMYSLGDDGD NDIEENSLNE SLLDGVTHSL DDLRLQSQKK GGELTEEQMK
     401  LKSALTKDAF VQIVKAATKN KHKVLAGEEE DDSKKTLHDD RESKSED*

external links for Cmk2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Cmk2p

1)	Pausch MH, et al. (1991) Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote. EMBO J 10(6):1511-22
2)	Ohya Y, et al. (1991) Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2. J Biol Chem 266(19):12784-94
3)	Cyert MS (2001) Genetic analysis of calmodulin and its targets in Saccharomyces cerevisiae. Annu Rev Genet 35:647-72
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41