TOP1/YOL006C Protein Information Help

Standard Name Top1p 1
Systematic Name Yol006cp
Alias Mak1p , Mak17p
ORF Classification Verified
Description Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination (1, 2, 3)
Name Description TOPoisomerase 1
Experimental Data
Molecules/cell 2970 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 769
Molecular Weight (Da) 89,995
Isoelectric Point (pI) 9.38

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Top1p (InterPro)
Physical Interactions There are 67 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000005300B | P04786
MIPS: YOL006C
NCBI: 135993 | 1419771 | 173004 | 253723032 | 6324568 | NP_014637.1 | NM_001183260.1
GenBank/EMBL/DDBJ: DAA10777.1 | K03077 | Z74748
External Classifications EC: 5.99.1.2 [DNA topoisomerase]
Amino Acid Sequence (or in FASTA format)
 
       1  MTIADASKVN HELSSDDDDD VPLSQTLKKR KVASMNSASL QDEAEPYDSD
      51  EAISKISKKK TKKIKTEPVQ SSSLPSPPAK KSATSKPKKI KKEDGDVKVK
     101  TTKKEEQENE KKKREEEEEE DKKAKEEEEE YKWWEKENED DTIKWVTLKH
     151  NGVIFPPPYQ PLPSHIKLYY DGKPVDLPPQ AEEVAGFFAA LLESDHAKNP
     201  VFQKNFFNDF LQVLKESGGP LNGIEIKEFS RCDFTKMFDY FQLQKEQKKQ
     251  LTSQEKKQIR LEREKFEEDY KFCELDGRRE QVGNFKVEPP DLFRGRGAHP
     301  KTGKLKRRVN PEDIVLNLSK DAPVPPAPEG HKWGEIRHDN TVQWLAMWRE
     351  NIFNSFKYVR LAANSSLKGQ SDYKKFEKAR QLKSYIDAIR RDYTRNLKSK
     401  VMLERQKAVA IYLIDVFALR AGGEKSEDEA DTVGCCSLRY EHVTLKPPNT
     451  VIFDFLGKDS IRFYQEVEVD KQVFKNLTIF KRPPKQPGHQ LFDRLDPSIL
     501  NKYLQNYMPG LTAKVFRTYN ASKTMQDQLD LIPNKGSVAE KILKYNAANR
     551  TVAILCNHQR TVTKGHAQTV EKANNRIQEL EWQKIRCKRA ILQLDKDLLK
     601  KEPKYFEEID DLTKEDEATI HKRIIDREIE KYQRKFVREN DKRKFEKEEL
     651  LPESQLKEWL EKVDEKKQEF EKELKTGEVE LKSSWNSVEK IKAQVEKLEQ
     701  RIQTSSIQLK DKEENSQVSL GTSKINYIDP RLSVVFCKKY DVPIEKIFTK
     751  TLREKFKWAI ESVDENWRF*

external links for Top1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Top1p
1) Thrash C, et al.  (1985) Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. Proc Natl Acad Sci U S A 82(13):4374-8
2) Roca J  (1995) The mechanisms of DNA topoisomerases. Trends Biochem Sci 20(4):156-60
3) Woo MH, et al.  (2003) Locking the DNA topoisomerase I protein clamp inhibits DNA rotation and induces cell lethality. Proc Natl Acad Sci U S A 100(24):13767-72
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41