PDR18/YNR070W Protein Information Help

Standard Name Pdr18p 1
Systematic Name Ynr070wp
ORF Classification Verified
Description Putative transporter of the ATP-binding cassette (ABC) family; role in plasma membrane sterol incorporation; implicated in pleiotropic drug resistance; provides resistance to ethanol stress and contributes to a decreased intracellular accumulation of ethanol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies (2, 3, 4, 5, 6)
Name Description Pleiotropic Drug Resistance
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,333
Molecular Weight (Da) 149,749
Isoelectric Point (pI) 7.91

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pdr18p (InterPro)
Transmembrane Domains There are 9 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 14 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013BACF | P53756
MIPS: YNR070W
NCBI: 1302604 | 1730699 | 2253198 | 398365893 | NP_014468.3 | NM_001183247.4
GenBank/EMBL/DDBJ: CAA96352.1 | CAA96354.1 | DAA10611.1 | Z71685 | Z71686
External Classifications TC: 3.A.1.205 [The Pleiotropic Drug Resistance (PDR) Family]
Amino Acid Sequence (or in FASTA format)
       1  MECVSVEGLD SSFLEGQTFG DILCLPWTII KGIRERKNRN KMKIILKNVS
      51  LLAKSGEMVL VLGRPGAGCT SFLKSAAGET SQFAGGVTTG HISYDGIPQK
     101  EMMQHYKPDV IYNGEQDVHF PHLTVKQTLD FAISCKMPAK RVNNVTKEEY
     151  ITANREFYAK IFGLTHTFDT KVGNDFISGV SGGERKRVSI AEALAAKGSI
     201  YCWDNATRGL DSSTALEFAR AIRTMTNLLG TTALVTVYQA SENIYETFDK
     251  VTVLYAGRQI FCGKTTEAKD YFENMGYLCP PRQSTAEYLT AITDPNGLHE
     301  IKPGFEYQVP HTADEFEKYW LDSPEYARLK GEIQKYKHEV NTEWTKKTYN
     351  ESMAQEKSKG TRKKSYYTVS YWEQIRLCTI RGFLRIYGDK SYTVINTCAA
     401  IAQAFITGSL FYQAPSSTLG AFSRSGVLFF SLLYYSLMGL ANISFEHRPI
     451  LQKHKVYSLY HPSAEALAST ISSFPFRMIG LTFFIIILYF LAGLHRSAGA
     501  FFTMYLLLTM CSEAITSLFQ MVSSLCDTLS QANSIAGVVM LSIAMYSTYM
     551  IQLPSMHPWF KWISYILPIR YAFESMLNAE FHGRHMDCGG TLVPSGPGFE
     601  NILPENQVCA FVGSRPGQSW VLGDDYLRAQ YQYEYKNTWR NFGIMWCFLI
     651  GYIVLRAVFT EYKSPVKSGG DALVVKKGTK NAIQRSWSSK NDEENLNASI
     701  ATQDMKEIAS SNDDSTSADF EGLESTGVFI WKNVSFTIPH SSGQRKLLDS
     751  VSGYCVPGTL TALIGESGAG KTTLLNTLAQ RNVGTITGDM LVDGLPMDAS
     801  FKRRTGYVQQ QDLHVAELTV KESLQFSARM RRPQSIPDAE KMEYVEKIIS
     851  ILEMQEFSEA LVGEIGYGLN VEQRKKLSIG VELVGKPDLL LFLDEPTSGL
     901  DSQSAWAVVK MLKRLALAGQ SILCTIHQPS ATLFEQFDRL LLLGKGGQTI
     951  YFGEIGKNSS SVIKYFEKNG ARKCQQNENP AEYILEAIGA GATASVQQNW
    1001  PDIWQKSHEY ANINEKINDM IKDLSSTTLH KTATRASKYA TSYSYQFHHV
    1051  LKRSSLTFWR NLNYIMAKMM LLMISGLFIG FTFFHVGVNA IGLQNSLFAC
    1101  FMAIVISAPA TNQIQERATV AKELYEVRES KSNMFHWSLL LITHYLNELP
    1151  YHLLFSTIFF VSSYFPLGVF TEASRSSVFY LNYAILFQLY YIGLALMILY
    1201  MSPNLQSANV IVGFILSFLL SFCGAVQPAS LMPGFWTFMW KLSPYTYFLQ
    1251  NLVGLLMHDK PVRCSKKELS LFNPPVGQTC GEFTKPFFEF GTGYIANPDA
    1301  TADCAYCQYK VGDEYLARIN ASFSYLWRNF GFI*                 

external links for Pdr18p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB CYC2008 (complexes) MIPS YeastGFP
YOGY Complexome Pfam domains

DIP TCDB

GeneMANIA YeastRC Structure Prediction (Seattle)

IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pdr18p
1) Rockwell NC, et al.  (2009) ABC transporter Pdr10 regulates the membrane microenvironment of Pdr12 in Saccharomyces cerevisiae. J Membr Biol 229(1):27-52
2) Bauer BE, et al.  (1999) Inventory and function of yeast ABC proteins: about sex, stress, pleiotropic drug and heavy metal resistance. Biochim Biophys Acta 1461(2):217-36
3) Decottignies A and Goffeau A  (1997) Complete inventory of the yeast ABC proteins. Nat Genet 15(2):137-45
4) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
5) Cabrito TR, et al.  (2011) The yeast ABC transporter Pdr18 (ORF YNR070w) controls plasma membrane sterol composition, playing a role in multidrug resistance. Biochem J 440(2):195-202
6) Teixeira MC, et al.  (2012) Increased expression of the yeast multidrug resistance ABC transporter Pdr18 leads to increased ethanol tolerance and ethanol production in high gravity alcoholic fermentation. Microb Cell Fact 11(1):98