FRE4/YNR060W Protein Information

Standard Name	Fre4p
Systematic Name	Ynr060wp
ORF Classification	Verified
Description	Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels (1, 2, 3)
Name Description	Ferric REductase

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	719
Molecular Weight (Da)	82,015
Isoelectric Point (pI)	9.96

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Fre4p (InterPro)
Transmembrane Domains	There are 5 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 3 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012ABF7 \| P53746 MIPS: YNR060W NCBI: 1302584 \| 1730689 \| 6324388 \| NP_014458.1 GenBank/EMBL/DDBJ: DAA10601.1 \| Z71675
External Classifications	EC: 1.16.1.7 [Ferric-chelate reductase] TC: 1.A.20.5

Amino Acid Sequence (or in FASTA format)
1 MLLVHIISFL LFFQLSAAKA PPSKTSLINT HERRSIYSCY VGLRKETWGF 51 NGSAICRYEP AIQSMLYCLY EDTHEKGYSN KTLEKGFEEM RQFCYTPKFL 101 NMTDAEFYTS LDNGTYYIQD QPKAGINITY PIRLNTTLRK AYYDAYYGYY 151 YNHDIPYYFG GIICAYFVGV MLLAGLIRFL NYTPIKKIMF QQKLVNYVRG 201 YTTLPTLYEK HAEPFSYLKV ITGYLPTRFE TLVILGYLIL HTIFMAYKYQ 251 YDPYHIIFAA HRAEVAHFVA YRSGILSFAH LPLIVLFAGR NNFLQLISGL 301 KHTSFIVFHK WLGRMMFLDA IIHAAGFTNY YLYYKKWNTV RLRVYWKFGI 351 ATTCLAGMLI FFSIAAFRRH YYETFMALHI VFAALFLYTC WEHVTNFSGI 401 EWIYAAIAIW GVDRIVRITR IALLGFPKAD LQLVGSDLVR VTVKKPKKFW 451 KAKPGQYVFV SFLRPLCFWQ SHPFTVMDSC VNDRELVIVL KAKKGVTKLV 501 RNFVERKGGK ASMRLAIEGP YGSKSTAHRF DNVLLLAGGS GLPGPISHAL 551 ELGKTTAASG KNFVQLVIAV RGLDMLNACK KELMALKGLN VQVHIYNSKQ 601 ELASAEKISS NEVKNGETTA EKAPSSLSNS EKAPSESENT ELPLSLNDTS 651 ISDLEFATFH VGRPNVEEIL NESVNHSGSL AVVCCGPPIF VDTARNQTAK 701 AVIRNPSRMI EYLEEYQAW*

Amino Acid Sequence (or in FASTA format)

       1  MLLVHIISFL LFFQLSAAKA PPSKTSLINT HERRSIYSCY VGLRKETWGF
      51  NGSAICRYEP AIQSMLYCLY EDTHEKGYSN KTLEKGFEEM RQFCYTPKFL
     101  NMTDAEFYTS LDNGTYYIQD QPKAGINITY PIRLNTTLRK AYYDAYYGYY
     151  YNHDIPYYFG GIICAYFVGV MLLAGLIRFL NYTPIKKIMF QQKLVNYVRG
     201  YTTLPTLYEK HAEPFSYLKV ITGYLPTRFE TLVILGYLIL HTIFMAYKYQ
     251  YDPYHIIFAA HRAEVAHFVA YRSGILSFAH LPLIVLFAGR NNFLQLISGL
     301  KHTSFIVFHK WLGRMMFLDA IIHAAGFTNY YLYYKKWNTV RLRVYWKFGI
     351  ATTCLAGMLI FFSIAAFRRH YYETFMALHI VFAALFLYTC WEHVTNFSGI
     401  EWIYAAIAIW GVDRIVRITR IALLGFPKAD LQLVGSDLVR VTVKKPKKFW
     451  KAKPGQYVFV SFLRPLCFWQ SHPFTVMDSC VNDRELVIVL KAKKGVTKLV
     501  RNFVERKGGK ASMRLAIEGP YGSKSTAHRF DNVLLLAGGS GLPGPISHAL
     551  ELGKTTAASG KNFVQLVIAV RGLDMLNACK KELMALKGLN VQVHIYNSKQ
     601  ELASAEKISS NEVKNGETTA EKAPSSLSNS EKAPSESENT ELPLSLNDTS
     651  ISDLEFATFH VGRPNVEEIL NESVNHSGSL AVVCCGPPIF VDTARNQTAK
     701  AVIRNPSRMI EYLEEYQAW*

external links for Fre4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	TCDB
	DIP	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Fre4p

1)	Martins LJ, et al. (1998) Metalloregulation of FRE1 and FRE2 homologs in Saccharomyces cerevisiae. J Biol Chem 273(37):23716-21
2)	Georgatsou E and Alexandraki D (1999) Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes. Yeast 15(7):573-84
3)	Yun CW, et al. (2001) The role of the FRE family of plasma membrane reductases in the uptake of siderophore-iron in Saccharomyces cerevisiae. J Biol Chem 276(13):10218-23