BIO4/YNR057C Protein Information

Standard Name	Bio4p 1
Systematic Name	Ynr057cp
ORF Classification	Verified
Description	Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels (1, 2)
Name Description	BIOtin 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	237
Molecular Weight (Da)	26,257
Isoelectric Point (pI)	4.9

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bio4p (InterPro)
Physical Interactions	There are 1 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001135E0 \| P53630 MIPS: YNR057C NCBI: 1297328 \| 1302578 \| 1705467 \| 45270738 \| 6324385 \| NP_014455.1 GenBank/EMBL/DDBJ: DAA10598.1 \| AY558424 \| U53467 \| Z71672
External Classifications	EC: 6.3.3.3 [Dethiobiotin synthase]

Amino Acid Sequence (or in FASTA format)
1 MNSKSQQQEQ QPIVFVTGTD TDVGKTFVST LLVHKWKAAY WKPVQTGIES 51 DQGDSETLKN FKIAASTWQP PIFTPTYALQ KPLSPLQAME YEPNVDIRLL 101 DFVVPEEWSA ENPLVVEGAG GVCVPITRKL EITTDLIKHL IETSGHPVYV 151 VVVARSGLGT LNHTLLTWNH LCDNGLRSHL FGVILNGEPN EGNVQALKKF 201 GVNIMAQVAQ CTTAHDQDME LHELPSVESL MTQQDVE*

Amino Acid Sequence (or in FASTA format)

       1  MNSKSQQQEQ QPIVFVTGTD TDVGKTFVST LLVHKWKAAY WKPVQTGIES
      51  DQGDSETLKN FKIAASTWQP PIFTPTYALQ KPLSPLQAME YEPNVDIRLL
     101  DFVVPEEWSA ENPLVVEGAG GVCVPITRKL EITTDLIKHL IETSGHPVYV
     151  VVVARSGLGT LNHTLLTWNH LCDNGLRSHL FGVILNGEPN EGNVQALKKF
     201  GVNIMAQVAQ CTTAHDQDME LHELPSVESL MTQQDVE*

external links for Bio4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Bio4p

1)	Phalip V, et al. (1999) Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake. Gene 232(1):43-51
2)	Shakoury-Elizeh M, et al. (2004) Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae. Mol Biol Cell 15(3):1233-43