HOL1/YNR055C Protein Information

Standard Name	Hol1p 1
Systematic Name	Ynr055cp
ORF Classification	Verified
Description	Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake (2, 3, 4, 5)
Name Description	HistidinOl 2

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	586
Molecular Weight (Da)	65,348
Isoelectric Point (pI)	5.56

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hol1p (InterPro)
Transmembrane Domains	There are 10 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 5 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012CAC1 \| P53389 MIPS: YNR055C NCBI: 1302574 \| 1708281 \| 2253197 \| 398365829 \| 825501 \| NP_014453.3 GenBank/EMBL/DDBJ: DAA10596.1 \| L42348 \| Z71670 \| Z71671
External Classifications	TC: 2.A.1.2 [The Drug:H+ Antiporter-1 (12 Spanner) (DHA1) Family]

Amino Acid Sequence (or in FASTA format)
1 MDKYTNRDHP DYIPGTFNIY SSQNLENGII YESKLKKTSS GVVLIPQPSY 51 SPNDPLNWSS WRKLAHFGLM AFITAFTAAT SNDAGAAQDS LNEIYGISYD 101 SMNTGAGVLF LGIGWSTLFL APFANLYGRK ITYIVCTTLG LFGALWFALA 151 KRTSDTIWSQ LFVGISESCA EAQVQLSLSD IFFQHQLGSV LTVYIMCTSI 201 GTFLGPLIAG YISAFTNFRW VGWVAVIISG GLLITIIFGC EETYFDRGQY 251 MTPLTSCQSG YEDGTTLQNS DNTAVSRRKR HLDAKLSTPG AMGEKGVDLS 301 ETAEFEVNNE EEVTIPETRE LIDGSKEHLK PYPKRVAILT KATNLKGYGF 351 KQYFKYLKIN LRMFLFPPVW LSGMFWGIQD VFLTFYLTTQ ESAYYEPPWN 401 YSDFGVAIMN VPTLIGAVIG CICAGIVSDY FVLWMARHNR GILEAEFRLY 451 FSIATAIIGP AGLLMFGIGT ARQWPWQAIY VGLGFVGFAW GCSGDIAMAY 501 LMDCYPDMVL EGMVCTAIIN NTISCIFTFT CSDWLAASGT ENTYIALAVI 551 NFGITAFALP MYYYGKRIRL WTKRWYLQSV NLRDGV*

Amino Acid Sequence (or in FASTA format)

       1  MDKYTNRDHP DYIPGTFNIY SSQNLENGII YESKLKKTSS GVVLIPQPSY
      51  SPNDPLNWSS WRKLAHFGLM AFITAFTAAT SNDAGAAQDS LNEIYGISYD
     101  SMNTGAGVLF LGIGWSTLFL APFANLYGRK ITYIVCTTLG LFGALWFALA
     151  KRTSDTIWSQ LFVGISESCA EAQVQLSLSD IFFQHQLGSV LTVYIMCTSI
     201  GTFLGPLIAG YISAFTNFRW VGWVAVIISG GLLITIIFGC EETYFDRGQY
     251  MTPLTSCQSG YEDGTTLQNS DNTAVSRRKR HLDAKLSTPG AMGEKGVDLS
     301  ETAEFEVNNE EEVTIPETRE LIDGSKEHLK PYPKRVAILT KATNLKGYGF
     351  KQYFKYLKIN LRMFLFPPVW LSGMFWGIQD VFLTFYLTTQ ESAYYEPPWN
     401  YSDFGVAIMN VPTLIGAVIG CICAGIVSDY FVLWMARHNR GILEAEFRLY
     451  FSIATAIIGP AGLLMFGIGT ARQWPWQAIY VGLGFVGFAW GCSGDIAMAY
     501  LMDCYPDMVL EGMVCTAIIN NTISCIFTFT CSDWLAASGT ENTYIALAVI
     551  NFGITAFALP MYYYGKRIRL WTKRWYLQSV NLRDGV*

external links for Hol1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	TCDB
YOGY	DIP	YeastRC Structure Prediction (Seattle)
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Hol1p

1)	Farabaugh, P. (1985) Personal Communication, Mortimer Map Edition 9
2)	Gaber RF, et al. (1990) HOL1 mutations confer novel ion transport in Saccharomyces cerevisiae. Mol Cell Biol 10(2):643-52
3)	Wright MB, et al. (1996) Amino acid substitutions in membrane-spanning domains of Hol1, a member of the major facilitator superfamily of transporters, confer nonselective cation uptake in Saccharomyces cerevisiae. J Bacteriol 178(24):7197-205
4)	Nelissen B, et al. (1995) Phylogenetic classification of the major superfamily of membrane transport facilitators, as deduced from yeast genome sequencing. FEBS Lett 377(2):232-6
5)	Dias PJ, et al. (2010) Evolution of the 12-Spanner Drug:H+ Antiporter DHA1 Family in Hemiascomycetous Yeasts. OMICS 14(6):701-10