POP2/YNR052C Protein Information

Standard Name	Pop2p
Systematic Name	Ynr052cp
Alias	Caf1p
ORF Classification	Verified
Description	RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation (1, 2, 3)
Name Description	PGK promoter directed OverProduction 4

Experimental Data
Molecules/cell	1520 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	433
Molecular Weight (Da)	49,682
Isoelectric Point (pI)	6.11

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pop2p (InterPro)
Physical Interactions	There are 132 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052DFA \| P39008 MIPS: YNR052C NCBI: 1302568 \| 1709720 \| 172080 \| 218463 \| 218465 \| 39654971 \| 39654972 \| 398365813 \| 422919281 \| 422919284 \| 422919286 \| 422919288 \| 422919289 \| 51013035 \| NP_014450.3 GenBank/EMBL/DDBJ: DAA10593.1 \| AY692792 \| D12807 \| D12808 \| M88607 \| Z71667
External Classifications	EC: 3.1.13.4 [Poly(A)-specific ribonuclease]

Amino Acid Sequence (or in FASTA format)
1 MQSMNVQPRV LAVGGEQFFS QRQASEQHQQ QNMGPQVYSP KVNRARMFPQ 51 GMPVNTINGS VNQEMNNAYL LKQKNEPLLT QQQQQQQQQQ QPFNIGTPVS 101 VASLPPGLNV LQQQQQQQQQ QQQQQQGVGL NRPLASQLPK HLTNQSMPPI 151 FLPPPNYLFV RDVWKSNLYS EFAVIRQLVS QYNHVSISTE FVGTLARPIG 201 TFRSKVDYHY QTMRANVDFL NPIQLGLSLS DANGNKPDNG PSTWQFNFEF 251 DPKKEIMSTE SLELLRKSGI NFEKHENLGI DVFEFSQLLM DSGLMMDDSV 301 TWITYHAAYD LGFLINILMN DSMPNNKEDF EWWVHQYMPN FYDLNLVYKI 351 IQEFKNPQLQ QSSQQQQQQQ YSLTTLADEL GLPRFSIFTT TGGQSLLMLL 401 SFCQLSKLSM HKFPNGTDFA KYQGVIYGID GDQ*

Amino Acid Sequence (or in FASTA format)

       1  MQSMNVQPRV LAVGGEQFFS QRQASEQHQQ QNMGPQVYSP KVNRARMFPQ
      51  GMPVNTINGS VNQEMNNAYL LKQKNEPLLT QQQQQQQQQQ QPFNIGTPVS
     101  VASLPPGLNV LQQQQQQQQQ QQQQQQGVGL NRPLASQLPK HLTNQSMPPI
     151  FLPPPNYLFV RDVWKSNLYS EFAVIRQLVS QYNHVSISTE FVGTLARPIG
     201  TFRSKVDYHY QTMRANVDFL NPIQLGLSLS DANGNKPDNG PSTWQFNFEF
     251  DPKKEIMSTE SLELLRKSGI NFEKHENLGI DVFEFSQLLM DSGLMMDDSV
     301  TWITYHAAYD LGFLINILMN DSMPNNKEDF EWWVHQYMPN FYDLNLVYKI
     351  IQEFKNPQLQ QSSQQQQQQQ YSLTTLADEL GLPRFSIFTT TGGQSLLMLL
     401  SFCQLSKLSM HKFPNGTDFA KYQGVIYGID GDQ*

external links for Pop2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pop2p

1)	Tucker M, et al. (2002) Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase complex in Saccharomyces cerevisiae. EMBO J 21(6):1427-36
2)	Daugeron MC, et al. (2001) The yeast POP2 gene encodes a nuclease involved in mRNA deadenylation. Nucleic Acids Res 29(12):2448-55
3)	Thore S, et al. (2003) X-ray structure and activity of the yeast Pop2 protein: a nuclease subunit of the mRNA deadenylase complex. EMBO Rep 4(12):1150-5
4)	Sakai A, et al. (1992) Molecular analysis of POP2 gene, a gene required for glucose-derepression of gene expression in Saccharomyces cerevisiae. Nucleic Acids Res 20(23):6227-33
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41