SSK2/YNR031C Protein Information

Standard Name	Ssk2p 1
Systematic Name	Ynr031cp
ORF Classification	Verified
Description	MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress; SSK2 has a paralog, SSK22, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description	Suppressor of Sensor Kinase 1

Experimental Data
Molecules/cell	217 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,579
Molecular Weight (Da)	180,526
Isoelectric Point (pI)	5.69

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ssk2p (InterPro)
Physical Interactions	There are 50 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000135FDB \| P53599 MIPS: YNR031C NCBI: 1302527 \| 1711541 \| 6324358 \| 940330 \| NP_014428.1 GenBank/EMBL/DDBJ: DAA10571.1 \| L41927 \| Z71646
External Classifications	EC: 2.7.11.25 [Mitogen-activated protein kinase kinase kinase]

Amino Acid Sequence (or in FASTA format)
1 MSHSDYFNYK PYGDSTEKPS SSKMRQSSSS SSSRLRSESL GRNSNTTQAR 51 VASSPISPGL HSTQYFRSPN AVYSPGESPL NTVQLFNRLP GIPQGQFFHQ 101 NAISGSSSSS ARSSRRPSNI GLPLPKNPQQ SLPKLSTQPV PVHKKVEASK 151 TESEIIKKPA PVNSNQDPLL TTPTLVISPE LASLNTTNTS IMSTPQNITN 201 QTSNKHIPTR SQPNGSTSSS TLQDIVTTNS SQRSVGHHGG STTSLRTYKK 251 QYVLNEQLYL RKMRNRANDD YYTRGIVASS NFEDDEENFS NKGEDDLELE 301 MDDLLKVEGE DKDNDFNFGY NFITSSTKNN ENVVSMSLNY LKGKLDWLRD 351 VNNDQPCEIE DEEWHSILGS EDLLSKLLQN PMVNNRFEWQ TMLSKVLKGD 401 IVRNEKTKIA NQGKGPGFNT QFSDDIWIEL KAWMNGRTVE DQNKSLRIFR 451 DSTDSVFQEI MAFKLEDNMS ADEAAETIKS LVDKYYRVLN LWPNIKRMHA 501 EKPITKTEAF RNRIDTLNSW LNFKFNFDTN IAYLKKWIVG NKELESTTEV 551 DNTTVNLDDP AVFATNCKRF AEQIMKEKDI ELIFQKKIFF PLAPWILKAK 601 FFFLKYQKTW NELNLSYLDQ DLEFLLMFPM RLVKDIILIR LSYAKKIQNP 651 TLMMIDQMMD DFSTYIKLAV QMKFTVASYC NDWFFKVKID PEFDHTVVEG 701 LEYFFSILEL RILYSGKNSF KTSKEPDLLL KYWEMFRNVG YYIDDAGELI 751 AAEFTKLTLR LVHRLHAYLL RQQNTPPKLE NEAAAEKWLV QIFEILGSMK 801 RKLNRFTNIL TKAFQNFVRY KIEDHNYLLK QLKETGHFLI YTGGYLEQNG 851 TYLIGSPELL GCKDDDILRI IKNSDIGCDL VPKLEINNSL TIYNALDDNW 901 NSNSSLGSDI SNDGTPFYYI KNDLTTQPRS YNGNRVNREP DFENSRSTEE 951 EFYELETRLN SLGYVLVLTP QEPLLWEGEM YNLSDNKTIK PEGLNLKVIP 1001 NSIDLMCQGS SYALEYQCDR FQQISGSSVS FLEKKSSSET VKNNLQRINK 1051 AYFRCTYSVL KNYTKIVTTF KKVSPVNDLL NNIFLFGRDF GLNFLRINVA 1101 NNEKRSIIIL LMMRLSIGWL KFLAEDCDPT DQRVFRWCVT SMEFAMHMVS 1151 GWNILALDEC QFSSLKQKIS ECMSLLISHF DIIGARSIEV EKINQQARSN 1201 LDLEDVFDDD MMLQVNSEFR VQSIMELEER IKRNPHQTGK VIDDSDKGNK 1251 YLVSLASSIS NVSMRWQKRN FIGGGTFGRV YSAVDLDNGE ILAVKEINIQ 1301 DSKSMQKIFP LIKEEMSVLE ILNHPNIVSY YGVEVHRDKV NIFMEYCEGG 1351 SLAALLEHGR IEDEMVTQVY TLQLLEGLAY LHESGIVHRD VKPENILLDF 1401 NGVIKYVDFG AAKKIANNGT RLASMNKIEN ADGEHEDVTH VSDSKAVKNN 1451 ENALLDMMGT PMYMAPESIT GSTTKGKLGA DDVWSLGCVV LEMITGRRPW 1501 ANLDNEWAIM YHVAAGHTPQ FPTKDEVSSA GMKFLERCLI QNPSKRASAV 1551 ELLMDPWIVQ IREIAFGDDS SSTDTEERE*

Amino Acid Sequence (or in FASTA format)

       1  MSHSDYFNYK PYGDSTEKPS SSKMRQSSSS SSSRLRSESL GRNSNTTQAR
      51  VASSPISPGL HSTQYFRSPN AVYSPGESPL NTVQLFNRLP GIPQGQFFHQ
     101  NAISGSSSSS ARSSRRPSNI GLPLPKNPQQ SLPKLSTQPV PVHKKVEASK
     151  TESEIIKKPA PVNSNQDPLL TTPTLVISPE LASLNTTNTS IMSTPQNITN
     201  QTSNKHIPTR SQPNGSTSSS TLQDIVTTNS SQRSVGHHGG STTSLRTYKK
     251  QYVLNEQLYL RKMRNRANDD YYTRGIVASS NFEDDEENFS NKGEDDLELE
     301  MDDLLKVEGE DKDNDFNFGY NFITSSTKNN ENVVSMSLNY LKGKLDWLRD
     351  VNNDQPCEIE DEEWHSILGS EDLLSKLLQN PMVNNRFEWQ TMLSKVLKGD
     401  IVRNEKTKIA NQGKGPGFNT QFSDDIWIEL KAWMNGRTVE DQNKSLRIFR
     451  DSTDSVFQEI MAFKLEDNMS ADEAAETIKS LVDKYYRVLN LWPNIKRMHA
     501  EKPITKTEAF RNRIDTLNSW LNFKFNFDTN IAYLKKWIVG NKELESTTEV
     551  DNTTVNLDDP AVFATNCKRF AEQIMKEKDI ELIFQKKIFF PLAPWILKAK
     601  FFFLKYQKTW NELNLSYLDQ DLEFLLMFPM RLVKDIILIR LSYAKKIQNP
     651  TLMMIDQMMD DFSTYIKLAV QMKFTVASYC NDWFFKVKID PEFDHTVVEG
     701  LEYFFSILEL RILYSGKNSF KTSKEPDLLL KYWEMFRNVG YYIDDAGELI
     751  AAEFTKLTLR LVHRLHAYLL RQQNTPPKLE NEAAAEKWLV QIFEILGSMK
     801  RKLNRFTNIL TKAFQNFVRY KIEDHNYLLK QLKETGHFLI YTGGYLEQNG
     851  TYLIGSPELL GCKDDDILRI IKNSDIGCDL VPKLEINNSL TIYNALDDNW
     901  NSNSSLGSDI SNDGTPFYYI KNDLTTQPRS YNGNRVNREP DFENSRSTEE
     951  EFYELETRLN SLGYVLVLTP QEPLLWEGEM YNLSDNKTIK PEGLNLKVIP
    1001  NSIDLMCQGS SYALEYQCDR FQQISGSSVS FLEKKSSSET VKNNLQRINK
    1051  AYFRCTYSVL KNYTKIVTTF KKVSPVNDLL NNIFLFGRDF GLNFLRINVA
    1101  NNEKRSIIIL LMMRLSIGWL KFLAEDCDPT DQRVFRWCVT SMEFAMHMVS
    1151  GWNILALDEC QFSSLKQKIS ECMSLLISHF DIIGARSIEV EKINQQARSN
    1201  LDLEDVFDDD MMLQVNSEFR VQSIMELEER IKRNPHQTGK VIDDSDKGNK
    1251  YLVSLASSIS NVSMRWQKRN FIGGGTFGRV YSAVDLDNGE ILAVKEINIQ
    1301  DSKSMQKIFP LIKEEMSVLE ILNHPNIVSY YGVEVHRDKV NIFMEYCEGG
    1351  SLAALLEHGR IEDEMVTQVY TLQLLEGLAY LHESGIVHRD VKPENILLDF
    1401  NGVIKYVDFG AAKKIANNGT RLASMNKIEN ADGEHEDVTH VSDSKAVKNN
    1451  ENALLDMMGT PMYMAPESIT GSTTKGKLGA DDVWSLGCVV LEMITGRRPW
    1501  ANLDNEWAIM YHVAAGHTPQ FPTKDEVSSA GMKFLERCLI QNPSKRASAV
    1551  ELLMDPWIVQ IREIAFGDDS SSTDTEERE*

external links for Ssk2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ssk2p

1)	Maeda T, et al. (1994) A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature 369(6477):242-5
2)	Posas F and Saito H (1998) Activation of the yeast SSK2 MAP kinase kinase kinase by the SSK1 two-component response regulator. EMBO J 17(5):1385-94
3)	Yuzyuk T, et al. (2002) The MEK kinase Ssk2p promotes actin cytoskeleton recovery after osmotic stress. Mol Biol Cell 13(8):2869-80
4)	Tatebayashi K, et al. (2003) A docking site determining specificity of Pbs2 MAPKK for Ssk2/Ssk22 MAPKKKs in the yeast HOG pathway. EMBO J 22(14):3624-34
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41