SEC12/YNR026C Protein Information

Standard Name	Sec12p 1
Systematic Name	Ynr026cp
Alias	Sed2p
ORF Classification	Verified
Description	Guanine nucleotide exchange factor (GEF); activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER; SEC12 has a paralog, SED4, that arose from the whole genome duplication (2, 3, 4)
Name Description	SECretory 5

Experimental Data
Molecules/cell	6160 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	471
Molecular Weight (Da)	51,607
Isoelectric Point (pI)	6.75

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sec12p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 6 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001355B2 \| P11655 MIPS: YNR026C NCBI: 1302517 \| 134263 \| 411024369 \| 411024370 \| 411024371 \| 411024372 \| 4435 \| 6324353 \| NP_014423.1 GenBank/EMBL/DDBJ: DAA10566.1 \| X13161 \| Z71641

Amino Acid Sequence (or in FASTA format)
1 MKFVTASYNV GYPAYGAKFL NNDTLLVAGG GGEGNNGIPN KLTVLRVDPT 51 KDTEKEQFHI LSEFALEDND DSPTAIDASK GIILVGCNEN STKITQGKGN 101 KHLRKFKYDK VNDQLEFLTS VDFDASTNAD DYTKLVYISR EGTVAAIASS 151 KVPAIMRIID PSDLTEKFEI ETRGEVKDLH FSTDGKVVAY ITGSSLEVIS 201 TVTGSCIARK TDFDKNWSLS KINFIADDTV LIAASLKKGK GIVLTKISIK 251 SGNTSVLRSK QVTNRFKGIT SMDVDMKGEL AVLASNDNSI ALVKLKDLSM 301 SKIFKQAHSF AITEVTISPD STYVASVSAA NTIHIIKLPL NYANYTSMKQ 351 KISKFFTNFI LIVLLSYILQ FSYKHNLHSM LFNYAKDNFL TKRDTISSPY 401 VVDEDLHQTT LFGNHGTKTS VPSVDSIKVH GVHETSSVNG TEVLCTESNI 451 INTGGAEFEI TNATFREIDD A*

Amino Acid Sequence (or in FASTA format)

       1  MKFVTASYNV GYPAYGAKFL NNDTLLVAGG GGEGNNGIPN KLTVLRVDPT
      51  KDTEKEQFHI LSEFALEDND DSPTAIDASK GIILVGCNEN STKITQGKGN
     101  KHLRKFKYDK VNDQLEFLTS VDFDASTNAD DYTKLVYISR EGTVAAIASS
     151  KVPAIMRIID PSDLTEKFEI ETRGEVKDLH FSTDGKVVAY ITGSSLEVIS
     201  TVTGSCIARK TDFDKNWSLS KINFIADDTV LIAASLKKGK GIVLTKISIK
     251  SGNTSVLRSK QVTNRFKGIT SMDVDMKGEL AVLASNDNSI ALVKLKDLSM
     301  SKIFKQAHSF AITEVTISPD STYVASVSAA NTIHIIKLPL NYANYTSMKQ
     351  KISKFFTNFI LIVLLSYILQ FSYKHNLHSM LFNYAKDNFL TKRDTISSPY
     401  VVDEDLHQTT LFGNHGTKTS VPSVDSIKVH GVHETSSVNG TEVLCTESNI
     451  INTGGAEFEI TNATFREIDD A*

external links for Sec12p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Sec12p

1)	Novick P, et al. (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15
2)	Barlowe C and Schekman R (1993) SEC12 encodes a guanine-nucleotide-exchange factor essential for transport vesicle budding from the ER. Nature 365(6444):347-9
3)	d'Enfert C, et al. (1991) Sec12p-dependent membrane binding of the small GTP-binding protein Sar1p promotes formation of transport vesicles from the ER. J Cell Biol 114(4):663-70
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Novick P and Schekman R (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76(4):1858-62
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41