SMM1/YNR015W Protein Information

Standard Name	Smm1p
Systematic Name	Ynr015wp
Alias	Dus2p
ORF Classification	Verified
Description	Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs (1, 2)
Name Description	Suppressor of Mitochondrial Mutation 3

Experimental Data
Molecules/cell	2650 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	384
Molecular Weight (Da)	42,816
Isoelectric Point (pI)	9.08

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Smm1p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000135AB0 \| P53720 MIPS: YNR015W NCBI: 1302496 \| 1616627 \| 1730664 \| 2253196 \| 6324342 \| NP_014412.1 \| NM_001183192.1 GenBank/EMBL/DDBJ: DAA10556.1 \| X91816 \| Z71630 \| Z71631
External Classifications	EC: 1.-.-.- [Oxidoreductases] EC: 1.3.1.91 [tRNA-dihydrouridine(20) synthase (NAD(P)(+))]

Amino Acid Sequence (or in FASTA format)
1 MVTYAGKLVL APMVRAGELP TRLMALAHGA DLVWSPEIID KKLIQCVRKE 51 NTALQTVDYV VPSKVQTRPE TLVFRTYPKL ESSKLIFQIG SASPALATQA 101 ALKVINDVSG IDINAGCPKH FSIHSGMGSA LLRTPDTLCL ILKELVKNVG 151 NPHSKPISVK IRLLDTKQDT LQLVKRLCAT GITNLTVHCR KTEMRNREQP 201 ITDYIAEIYE ICQANNVSLI VNGAIRDRSH FHDLQANHWK NTNIGGMIAE 251 CAERDPTVFD HTSKPSEDGP SWVVACREFI QWATKFDNHI GNTKYMLSRI 301 VPGKSVFFQY FARCKSPEEV SFVLKQLNDD GSAQTDPSEY LENCRAQEKA 351 LKNANAIAKQ KRKQTDHIGS DTKKQKVVPL PTDI*

Amino Acid Sequence (or in FASTA format)

       1  MVTYAGKLVL APMVRAGELP TRLMALAHGA DLVWSPEIID KKLIQCVRKE
      51  NTALQTVDYV VPSKVQTRPE TLVFRTYPKL ESSKLIFQIG SASPALATQA
     101  ALKVINDVSG IDINAGCPKH FSIHSGMGSA LLRTPDTLCL ILKELVKNVG
     151  NPHSKPISVK IRLLDTKQDT LQLVKRLCAT GITNLTVHCR KTEMRNREQP
     201  ITDYIAEIYE ICQANNVSLI VNGAIRDRSH FHDLQANHWK NTNIGGMIAE
     251  CAERDPTVFD HTSKPSEDGP SWVVACREFI QWATKFDNHI GNTKYMLSRI
     301  VPGKSVFFQY FARCKSPEEV SFVLKQLNDD GSAQTDPSEY LENCRAQEKA
     351  LKNANAIAKQ KRKQTDHIGS DTKKQKVVPL PTDI*

external links for Smm1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Smm1p

1)	Xing F, et al. (2002) A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA. RNA 8(3):370-81
2)	Xing F, et al. (2004) The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs. J Biol Chem 279(17):17850-60
3)	Rinaldi T, et al. (2003) Nucleo-mitochondrial interactions in Saccharomyces cerevisiae: characterization of a nuclear gene suppressing a defect in mitochondrial tRNA(Asp) processing. Gene 303():63-8
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41