PRP2/YNR011C Protein Information Help

Standard Name Prp2p
Systematic Name Ynr011cp
Alias Rna2p
ORF Classification Verified
Description RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 (1, 2)
Name Description Pre-mRNA Processing 3
Experimental Data
Molecules/cell 172 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 876
Molecular Weight (Da) 99,813
Isoelectric Point (pI) 8.42

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Prp2p (InterPro)
Physical Interactions There are 66 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000531F7 | P20095
MIPS: YNR011C
NCBI: 1302488 | 130804 | 4232 | 4237 | 496727 | 6324338 | NP_014408.1
GenBank/EMBL/DDBJ: DAA10552.1 | X55936 | X55999 | X77395 | Z71626
External Classifications EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]
EC: 3.6.4.13 [RNA helicase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSSITSETGK RRVKRTYEVT RQNDNAVRIE PSSLGEEEDK EAKDKNSALQ
      51  LKRSRYDPNK VFSNTNQGPE KNNLKGEQLG SQKKSSKYDE KITSNNELTT
     101  KKGLLGDSEN ETKYASSNSK FNVEVTHKIK NAKEIDKINR QRMWEEQQLR
     151  NAMAGQSDHP DDITLEGSDK YDYVFDTDAM IDYTNEEDDL LPEEKLQYEA
     201  RLAQALETEE KRILTIQEAR KLLPVHQYKD ELLQEIKKNQ VLIIMGETGS
     251  GKTTQLPQYL VEDGFTDQGK LQIAITQPRR VAATSVAARV ADEMNVVLGK
     301  EVGYQIRFED KTTPNKTVLK YMTDGMLLRE FLTDSKLSKY SCIMIDEAHE
     351  RTLATDILIG LLKDILPQRP TLKLLISSAT MNAKKFSEFF DNCPIFNVPG
     401  RRYPVDIHYT LQPEANYIHA AITTIFQIHT TQSLPGDILV FLTGQEEIER
     451  TKTKLEEIMS KLGSRTKQMI ITPIYANLPQ EQQLKIFQPT PENCRKVVLA
     501  TNIAETSLTI DGIRYVIDPG FVKENSYVPS TGMTQLLTVP CSRASVDQRA
     551  GRAGRVGPGK CFRIFTKWSY LHELELMPKP EITRTNLSNT VLLLLSLGVT
     601  DLIKFPLMDK PSIPTLRKSL ENLYILGALN SKGTITRLGK MMCEFPCEPE
     651  FAKVLYTAAT HEQCQGVLEE CLTIVSMLHE TPSLFIGQKR DAAASVLSEV
     701  ESDHILYLEI FNQWRNSKFS RSWCQDHKIQ FKTMLRVRNI RNQLFRCSEK
     751  VGLVEKNDQA RMKIGNIAGY INARITRCFI SGFPMNIVQL GPTGYQTMGR
     801  SSGGLNVSVH PTSILFVNHK EKAQRPSKYV LYQQLMLTSK EFIRDCLVIP
     851  KEEWLIDMVP QIFKDLIDDK TNRGRR*

external links for Prp2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Prp2p
1) Kim SH and Lin RJ  (1996) Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing. Mol Cell Biol 16(12):6810-9
2) Gencheva M, et al.  (2010) Nuclear Retention of Unspliced Pre-mRNAs by Mutant DHX16/hPRP2, a Spliceosomal DEAH-box Protein. J Biol Chem 285(46):35624-32
3) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41