ATG3/YNR007C Protein Information

Standard Name	Atg3p 1
Systematic Name	Ynr007cp
Alias	Apg3p 2 , Aut1p 3
ORF Classification	Verified
Description	E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p (2, 4, 5, 6)
Name Description	AuTophaGy related 1

Experimental Data
Molecules/cell	3610 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	310
Molecular Weight (Da)	35,887
Isoelectric Point (pI)	4.37

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Atg3p (InterPro)
Physical Interactions	There are 41 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052F5F \| P40344 MIPS: YNR007C NCBI: 126030345 \| 1302480 \| 358009653 \| 358009654 \| 398365567 \| 414145780 \| 414145781 \| 496724 \| 732206 \| NP_014404.3 GenBank/EMBL/DDBJ: DAA10548.1 \| X77395 \| Z71622

Amino Acid Sequence (or in FASTA format)
1 MIRSTLSSWR EYLTPITHKS TFLTTGQITP EEFVQAGDYL CHMFPTWKWN 51 EESSDISYRD FLPKNKQFLI IRKVPCDKRA EQCVEVEGPD VIMKGFAEDG 101 DEDDVLEYIG SETEHVQSTP AGGTKDSSID DIDELIQDME IKEEDENDDT 151 EEFNAKGGLA KDMAQERYYD LYIAYSTSYR VPKMYIVGFN SNGSPLSPEQ 201 MFEDISADYR TKTATIEKLP FYKNSVLSVS IHPCKHANVM KILLDKVRVV 251 RQRRRKELQE EQELDGVGDW EDLQDDIDDS LRVDQYLIVF LKFITSVTPS 301 IQHDYTMEGW *

Amino Acid Sequence (or in FASTA format)

       1  MIRSTLSSWR EYLTPITHKS TFLTTGQITP EEFVQAGDYL CHMFPTWKWN
      51  EESSDISYRD FLPKNKQFLI IRKVPCDKRA EQCVEVEGPD VIMKGFAEDG
     101  DEDDVLEYIG SETEHVQSTP AGGTKDSSID DIDELIQDME IKEEDENDDT
     151  EEFNAKGGLA KDMAQERYYD LYIAYSTSYR VPKMYIVGFN SNGSPLSPEQ
     201  MFEDISADYR TKTATIEKLP FYKNSVLSVS IHPCKHANVM KILLDKVRVV
     251  RQRRRKELQE EQELDGVGDW EDLQDDIDDS LRVDQYLIVF LKFITSVTPS
     301  IQHDYTMEGW *

external links for Atg3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Atg3p

1)	Klionsky DJ, et al. (2003) A unified nomenclature for yeast autophagy-related genes. Dev Cell 5(4):539-45
2)	Tsukada M and Ohsumi Y (1993) Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Lett 333(1-2):169-74
3)	Thumm M, et al. (1994) Isolation of autophagocytosis mutants of Saccharomyces cerevisiae. FEBS Lett 349(2):275-80
4)	Schlumpberger M, et al. (1997) AUT1, a gene essential for autophagocytosis in the yeast Saccharomyces cerevisiae. J Bacteriol 179(4):1068-76
5)	Ichimura Y, et al. (2000) A ubiquitin-like system mediates protein lipidation. Nature 408(6811):488-92
6)	Yi C, et al. (2012) Function and molecular mechanism of acetylation in autophagy regulation. Science 336(6080):474-7
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41