PFS2/YNL317W Protein Information

Standard Name	Pfs2p
Systematic Name	Ynl317wp
ORF Classification	Verified
Description	Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex (1, 2)
Name Description	Polyadenylation Factor Subunit 3

Experimental Data
Molecules/cell	7810 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	465
Molecular Weight (Da)	53,143
Isoelectric Point (pI)	8.16

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pfs2p (InterPro)
Physical Interactions	There are 121 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052DDC \| P42841 MIPS: YNL317W NCBI: 1176585 \| 1302427 \| 51013567 \| 6324012 \| 633666 \| NP_014082.1 GenBank/EMBL/DDBJ: DAA10244.1 \| AY693058 \| Z46259 \| Z71593

Amino Acid Sequence (or in FASTA format)
1 MDGHNQNQYQ NQNQIQQSQQ PPLKKYVTQR RSVDVSSPYI NLYYNRRHGL 51 PNLVVEPETS YTIDIMPPNA YRGRDRVINL PSKFTHLSSN KVKHVIPAIQ 101 WTPEGRRLVV ATYSGEFSLW NASSFTFETL MQAHDSAVTT MKYSHDSDWM 151 ISGDADGMIK IWQPNFSMVK EIDAAHTESI RDMAFSSNDS KFVTCSDDNI 201 LKIWNFSNGK QERVLSGHHW DVKSCDWHPE MGLIASASKD NLVKLWDPRS 251 GNCISSILKF KHTVLKTRFQ PTKGNLLMAI SKDKSCRVFD IRYSMKELMC 301 VRDETDYMTL EWHPINESMF TLACYDGSLK HFDLLQNLNE PILTIPYAHD 351 KCITSLSYNP VGHIFATAAK DRTIRFWTRA RPIDPNAYDD PTYNNKKING 401 WFFGINNDIN AVREKSEFGA APPPPATLEP HALPNMNGFI NKKPRQEIPG 451 IDSNIKSSTL PGLSI*

Amino Acid Sequence (or in FASTA format)

       1  MDGHNQNQYQ NQNQIQQSQQ PPLKKYVTQR RSVDVSSPYI NLYYNRRHGL
      51  PNLVVEPETS YTIDIMPPNA YRGRDRVINL PSKFTHLSSN KVKHVIPAIQ
     101  WTPEGRRLVV ATYSGEFSLW NASSFTFETL MQAHDSAVTT MKYSHDSDWM
     151  ISGDADGMIK IWQPNFSMVK EIDAAHTESI RDMAFSSNDS KFVTCSDDNI
     201  LKIWNFSNGK QERVLSGHHW DVKSCDWHPE MGLIASASKD NLVKLWDPRS
     251  GNCISSILKF KHTVLKTRFQ PTKGNLLMAI SKDKSCRVFD IRYSMKELMC
     301  VRDETDYMTL EWHPINESMF TLACYDGSLK HFDLLQNLNE PILTIPYAHD
     351  KCITSLSYNP VGHIFATAAK DRTIRFWTRA RPIDPNAYDD PTYNNKKING
     401  WFFGINNDIN AVREKSEFGA APPPPATLEP HALPNMNGFI NKKPRQEIPG
     451  IDSNIKSSTL PGLSI*

external links for Pfs2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Pfs2p

1)	Ohnacker M, et al. (2000) The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex. EMBO J 19(1):37-47
2)	Vo LT, et al. (2001) Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA. Mol Cell Biol 21(24):8346-56
3)	Keller W and Minvielle-Sebastia L (1997) A comparison of mammalian and yeast pre-mRNA 3'-end processing. Curr Opin Cell Biol 9(3):329-36
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41