MCK1/YNL307C Protein Information Help

Standard Name Mck1p 1
Systematic Name Ynl307cp
Alias Ypk1p 2 , Cms1p 1, 3
ORF Classification Verified
Description Dual-specificity serine/threonine and tyrosine protein kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family; MCK1 has a paralog, YGK3, that arose from the whole genome duplication (1, 4, 5, 6, 7)
Name Description Meiotic and Centromere regulatory ser, tyr-Kinase
Experimental Data
Molecules/cell 396 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 375
Molecular Weight (Da) 43,136
Isoelectric Point (pI) 8.92

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mck1p (InterPro)
Physical Interactions There are 183 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012ED92 | P21965
MIPS: YNL307C
NCBI: 126820 | 1302407 | 171910 | 2253188 | 3908 | 6324022 | 633675 | NP_014092.1
GenBank/EMBL/DDBJ: DAA10254.1 | M55984 | X55054 | Z46259 | Z71582 | Z71583
External Classifications EC: 2.7.12.1 [Dual-specificity kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSTEEQNGVP LQRGSEFIAD DVTSNKSNNT RRMLVKEYRK IGRGAFGTVV
      51  QAYLTQDKKN WLGPFAIKKV PAHTEYKSRE LQILRIADHP NIVKLQYFFT
     101  HLSPQDNKVY QHLAMECLPE TLQIEINRYV TNKLEMPLKH IRLYTYQIAR
     151  GMLYLHGLGV CHRDIKPSNV LVDPETGVLK ICDFGSAKKL EHNQPSISYI
     201  CSRFYRAPEL IIGCTQYTTQ IDIWGLGCVM GEMLIGKAIF QGQEPLLQLR
     251  EIAKLLGPPD KRFIFFSNPA YDGPLFSKPL FSGSSQQRFE KYFGHSGPDG
     301  IDLLMKILVY EPQQRLSPRR ILAHQFFNEL RNDDTFLPRG FTEPIKLPNL
     351  FDFNDFELQI LGEFADKIKP TKVAE*

external links for Mck1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Mck1p
1) Shero JH and Hieter P  (1991) A suppressor of a centromere DNA mutation encodes a putative protein kinase (MCK1). Genes Dev 5(4):549-60
2) Dailey D, et al.  (1990) Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity. Mol Cell Biol 10(12):6244-56
3) Schero, J.  (1992) Personal Communication, Mortimer Map Edition 11
4) Lim MY, et al.  (1993) Yeast MCK1 protein kinase autophosphorylates at tyrosine and serine but phosphorylates exogenous substrates at serine and threonine. J Biol Chem 268(28):21155-64
5) Neigeborn L and Mitchell AP  (1991) The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression. Genes Dev 5(4):533-48
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) McQueen J, et al.  (2012) The Mck1 GSK-3 kinase inhibits the activity of Clb2-Cdk1 post-nuclear division. Cell Cycle 11(18):3421-32
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41