CLA4/YNL298W Protein Information

Standard Name	Cla4p 1
Systematic Name	Ynl298wp
Alias	Erc10p 1, 2, 3
ORF Classification	Verified
Description	Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7, 8)
Name Description	CLn Activity dependant 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	842
Molecular Weight (Da)	93,908
Isoelectric Point (pI)	9.81

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cla4p (InterPro)
Physical Interactions	There are 104 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000127A83 \| P48562 MIPS: YNL298W NCBI: 1050862 \| 1302390 \| 1346390 \| 6324031 \| 732944 \| NP_014101.1 \| NM_001183136.1 GenBank/EMBL/DDBJ: DAA10262.1 \| U23084 \| X82499 \| Z71574
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MSLSAAANKI SDNDFQNIGP APRPPSSNSQ GRTCYNQTQP ITKLMSQLDL 51 TSASHLGTST SKKKSGWVSY KDDGILSFIW QKRYLMLHDS YVALYKNDKQ 101 NDDAILKIPL TSIISVSRTQ LKQYCFELVR CSDRNSVSSG SSSSLNVSSD 151 SNSKKSIYIA TKTESDLHSW LDAIFAKCPL LSGVSSPTNF THKVHVGFDP 201 ETGSFVGMPT NWEKLLKHSR ITGEDWNNNS AAVIQVLQFY QEYNGAGNPT 251 NTLDKPQSGE TSSSQKSLPN SYNDNKLRNN SVNSKSSSGV SSSMVSQRKT 301 SQPPNTKSPV SLGSGSLPPI NTKLPTSQSN IPRHLQNVPN QQYPKMRNGH 351 SPTNGQFPRG PMHPNNSQRS LQQQQQQQQQ QKQQHQQYPY HHQGPSPSPS 401 PSPSPLNPYR PHHNMINPYS KQPQSPLSSQ STQNQAIPRY AQNSSPTAAH 451 FQPQRTAPKP PISAPRAPYP SNQNATSNTH VQPVAPKNDQ STPQTMRQAP 501 KRPDADVAQP GGVAKPKKPA RPTMSTAEIM SKLKKVTVNA DPSQCFKVIE 551 KAGQGASGSV YLAERTHIPT ESNMIELINN DIDEPHVGDK VAIKQMVLSK 601 QPRKELIVNE ILVMKDSRHK NIVNFLEAYL RTDDDLWVVM EFMEGGSLTD 651 IIENSPTNDN SHSPLTEPQI AYIVRETCQG LKFLHDKHII HRDIKSDNVL 701 LDTRARVKIT DFGFCARLTD KRSKRATMVG TPYWMAPEVV KQREYDEKID 751 VWSLGIMTIE MLEGEPPYLN EDPLKALYLI ATNGTPKLKH PESLSLEIKR 801 FLSVCLCVDV RYRASTEELL HHGFFNMACD PKDLTSLLEW KE*

Amino Acid Sequence (or in FASTA format)

       1  MSLSAAANKI SDNDFQNIGP APRPPSSNSQ GRTCYNQTQP ITKLMSQLDL
      51  TSASHLGTST SKKKSGWVSY KDDGILSFIW QKRYLMLHDS YVALYKNDKQ
     101  NDDAILKIPL TSIISVSRTQ LKQYCFELVR CSDRNSVSSG SSSSLNVSSD
     151  SNSKKSIYIA TKTESDLHSW LDAIFAKCPL LSGVSSPTNF THKVHVGFDP
     201  ETGSFVGMPT NWEKLLKHSR ITGEDWNNNS AAVIQVLQFY QEYNGAGNPT
     251  NTLDKPQSGE TSSSQKSLPN SYNDNKLRNN SVNSKSSSGV SSSMVSQRKT
     301  SQPPNTKSPV SLGSGSLPPI NTKLPTSQSN IPRHLQNVPN QQYPKMRNGH
     351  SPTNGQFPRG PMHPNNSQRS LQQQQQQQQQ QKQQHQQYPY HHQGPSPSPS
     401  PSPSPLNPYR PHHNMINPYS KQPQSPLSSQ STQNQAIPRY AQNSSPTAAH
     451  FQPQRTAPKP PISAPRAPYP SNQNATSNTH VQPVAPKNDQ STPQTMRQAP
     501  KRPDADVAQP GGVAKPKKPA RPTMSTAEIM SKLKKVTVNA DPSQCFKVIE
     551  KAGQGASGSV YLAERTHIPT ESNMIELINN DIDEPHVGDK VAIKQMVLSK
     601  QPRKELIVNE ILVMKDSRHK NIVNFLEAYL RTDDDLWVVM EFMEGGSLTD
     651  IIENSPTNDN SHSPLTEPQI AYIVRETCQG LKFLHDKHII HRDIKSDNVL
     701  LDTRARVKIT DFGFCARLTD KRSKRATMVG TPYWMAPEVV KQREYDEKID
     751  VWSLGIMTIE MLEGEPPYLN EDPLKALYLI ATNGTPKLKH PESLSLEIKR
     801  FLSVCLCVDV RYRASTEELL HHGFFNMACD PKDLTSLLEW KE*

external links for Cla4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cla4p

1)	Cvrckova F, et al. (1995) Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast. Genes Dev 9(15):1817-30
2)	Benton BK, et al. (1993) Genetic analysis of Cln/Cdc28 regulation of cell morphogenesis in budding yeast. EMBO J 12(13):5267-75
3)	Benton BK, et al. (1997) Cla4p, a Saccharomyces cerevisiae Cdc42p-activated kinase involved in cytokinesis, is activated at mitosis. Mol Cell Biol 17(9):5067-76
4)	Caviston JP, et al. (2003) The role of Cdc42p GTPase-activating proteins in assembly of the septin ring in yeast. Mol Biol Cell 14(10):4051-66
5)	Versele M and Thorner J (2004) Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4. J Cell Biol 164(5):701-15
6)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7)	Bartholomew CR and Hardy CF (2009) p21-activated kinases Cla4 and Ste20 regulate vacuole inheritance in Saccharomyces cerevisiae. Eukaryot Cell 8(4):560-72
8)	Lin M, et al. (2009) The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway. Mol Biol Cell 20(22):4826-37
9)	Cvrckova F and Nasmyth K (1993) Yeast G1 cyclins CLN1 and CLN2 and a GAP-like protein have a role in bud formation. EMBO J 12(13):5277-86