MON2/YNL297C Protein Information

Standard Name	Mon2p
Systematic Name	Ynl297cp
Alias	Ysl2p
ORF Classification	Verified
Description	Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins (1)
Name Description	MONensin sensitivity 2

Experimental Data
Molecules/cell	2410 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,636
Molecular Weight (Da)	186,834
Isoelectric Point (pI)	5.47

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mon2p (InterPro)
Physical Interactions	There are 18 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013BA7F \| P48563 MIPS: YNL297C NCBI: 1050863 \| 1302387 \| 1353105 \| 6324033 \| NP_014102.1 \| NM_001183135.1 GenBank/EMBL/DDBJ: DAA10263.1 \| U23084 \| Z71573

Amino Acid Sequence (or in FASTA format)
1 MAMNTGGFDS MQRQLEAELR SLSSESKRRN STIRHASDKS IEILKRVHSF 51 EELERHPDFA LPFVLACQSR NAKMTTLAMQ CLQGLSTVPS IPRSRLSEIL 101 DAFIEATHLA MEIQLKVLQV VPIFFKTYGK FIYGPLCKKL LLCCSNLLHV 151 PNKAPVVVGT ASATLQQLID EIFDRLSIES VVDDKQYEVL ISNSESIKVN 201 VYRYDANKLF DNICSLNEIS SNGAVSDEEM LLDIGDIPID YGLEILESIL 251 KNSQKNLLEC QDLQYLLRVK AIPLLLRCIS SSRHFSTAVR SCRCLKLLIR 301 KEYLSILELE LEVILSLLIH GISVESNLSG WQRVLSLELF KDLSQDPEIV 351 NTLYMDYDNY PDKKHVFKYL LKECIVLLNS PEYITFLAPS KVVEKMDSPL 401 ITTENSTVKT KFMHLLDKSN APSINITYII SLILTICNHL CEGLNKSALE 451 SSPLEKKIED KEREEGTGND STVVKVYSGL FSGLFELNKL FLYSTSLETS 501 IFHLVVRAFQ KLAHSAGVLS LKDKLRACMK LFSILITNNV TSSNQYSFND 551 TSKSAKNQHT RNISTSSVTT SPVESTKNPS RSIADSAQNK EMKRRLHPRN 601 ISSRQVSLLR ALISLSISLG PIFDSESWRY TFLTWQWITY YIYGPSADFK 651 ESFYSEDIPP PPILTKSDVT SIESSLTKFF ESTSSYSCST FHLVLTRLIL 701 DSKNTLTLEQ TNLNLNNDIG YHPLDAKDEI IPCIYNKAFF VNKIGELATY 751 NCKKFLFGKN GKELWSLIST YMIKLISNRE MDNDSLRLYT VRVFTDIIKK 801 ATNEVGNSDE QDNKVKQFGT LENLVIDSLM ATINSIKQLD IGKQEIYNGT 851 INVESDILFQ LLLTLKEILN EFGELLMNSW TNIFNIINSP FEWTVEDTDF 901 SVNEDIDDSS LFEGIVQKHK NMIQVSYDVF KLISDDFLQS LPMSVIKFVI 951 DTLVNFVSQK RNLNISFSSI SQFWLVGDYL RVRFNPETLN LSDEKRRSLS 1001 EKINNQKLIE IITSSSSHDW ELYNGLWIYL LKNLINCTND DRVEVKNGAV 1051 QTFFRIIDSH SVCFPPWDLI FLEVIEPLLT KEWSTEELEN ETDFINVTLQ 1101 GLIKLYPEHF KDFKNNTTCA KEWSMLLDFL KRLLSSTSNN TKNAVILNYQ 1151 TLLKEIITIE DVPSDILKKC CEIFTDYNIT YSDLSTNASS KTEYDCIYEL 1201 ITGFPPLYQL ISKYDAMTDE FVEKVLLLFN SAIKYPLLPE FVQDKTKPSS 1251 MQKAILSGLD IFMTNDSKDT EILILLQLST ISILAFDTRE KITKKLGPKL 1301 PKASLNRLPT FEAISYMSCS NLRNRIAKID QFGISTLKAK HILRILKNLA 1351 EIIKRKSLIT GSESDEIPIW VLASNCFCDL SNKIFKSLQE DAENPLKDNF 1401 CDLFINVIVV TLQRINPELD NLTEIDDLNE YSKYREILLE NRIIDLFNER 1451 QLDTFIYAVW DCSFLYEFDE LENALMQDCG TFSELSQKLS SFDFSCIFGS 1501 TTNPRFQTKY KCSLECLQDL VNFMLNTNEK LRKLTAPYLS ARIALALRRY 1551 ISDEYLIGRA PIPKLRKTEL ATLLNGLCVI LRGVLDQNST LGNKQIGVEN 1601 LQTLSPLILR TIPVSHKMDG LQDKVLELSL GFTKLD*

Amino Acid Sequence (or in FASTA format)

       1  MAMNTGGFDS MQRQLEAELR SLSSESKRRN STIRHASDKS IEILKRVHSF
      51  EELERHPDFA LPFVLACQSR NAKMTTLAMQ CLQGLSTVPS IPRSRLSEIL
     101  DAFIEATHLA MEIQLKVLQV VPIFFKTYGK FIYGPLCKKL LLCCSNLLHV
     151  PNKAPVVVGT ASATLQQLID EIFDRLSIES VVDDKQYEVL ISNSESIKVN
     201  VYRYDANKLF DNICSLNEIS SNGAVSDEEM LLDIGDIPID YGLEILESIL
     251  KNSQKNLLEC QDLQYLLRVK AIPLLLRCIS SSRHFSTAVR SCRCLKLLIR
     301  KEYLSILELE LEVILSLLIH GISVESNLSG WQRVLSLELF KDLSQDPEIV
     351  NTLYMDYDNY PDKKHVFKYL LKECIVLLNS PEYITFLAPS KVVEKMDSPL
     401  ITTENSTVKT KFMHLLDKSN APSINITYII SLILTICNHL CEGLNKSALE
     451  SSPLEKKIED KEREEGTGND STVVKVYSGL FSGLFELNKL FLYSTSLETS
     501  IFHLVVRAFQ KLAHSAGVLS LKDKLRACMK LFSILITNNV TSSNQYSFND
     551  TSKSAKNQHT RNISTSSVTT SPVESTKNPS RSIADSAQNK EMKRRLHPRN
     601  ISSRQVSLLR ALISLSISLG PIFDSESWRY TFLTWQWITY YIYGPSADFK
     651  ESFYSEDIPP PPILTKSDVT SIESSLTKFF ESTSSYSCST FHLVLTRLIL
     701  DSKNTLTLEQ TNLNLNNDIG YHPLDAKDEI IPCIYNKAFF VNKIGELATY
     751  NCKKFLFGKN GKELWSLIST YMIKLISNRE MDNDSLRLYT VRVFTDIIKK
     801  ATNEVGNSDE QDNKVKQFGT LENLVIDSLM ATINSIKQLD IGKQEIYNGT
     851  INVESDILFQ LLLTLKEILN EFGELLMNSW TNIFNIINSP FEWTVEDTDF
     901  SVNEDIDDSS LFEGIVQKHK NMIQVSYDVF KLISDDFLQS LPMSVIKFVI
     951  DTLVNFVSQK RNLNISFSSI SQFWLVGDYL RVRFNPETLN LSDEKRRSLS
    1001  EKINNQKLIE IITSSSSHDW ELYNGLWIYL LKNLINCTND DRVEVKNGAV
    1051  QTFFRIIDSH SVCFPPWDLI FLEVIEPLLT KEWSTEELEN ETDFINVTLQ
    1101  GLIKLYPEHF KDFKNNTTCA KEWSMLLDFL KRLLSSTSNN TKNAVILNYQ
    1151  TLLKEIITIE DVPSDILKKC CEIFTDYNIT YSDLSTNASS KTEYDCIYEL
    1201  ITGFPPLYQL ISKYDAMTDE FVEKVLLLFN SAIKYPLLPE FVQDKTKPSS
    1251  MQKAILSGLD IFMTNDSKDT EILILLQLST ISILAFDTRE KITKKLGPKL
    1301  PKASLNRLPT FEAISYMSCS NLRNRIAKID QFGISTLKAK HILRILKNLA
    1351  EIIKRKSLIT GSESDEIPIW VLASNCFCDL SNKIFKSLQE DAENPLKDNF
    1401  CDLFINVIVV TLQRINPELD NLTEIDDLNE YSKYREILLE NRIIDLFNER
    1451  QLDTFIYAVW DCSFLYEFDE LENALMQDCG TFSELSQKLS SFDFSCIFGS
    1501  TTNPRFQTKY KCSLECLQDL VNFMLNTNEK LRKLTAPYLS ARIALALRRY
    1551  ISDEYLIGRA PIPKLRKTEL ATLLNGLCVI LRGVLDQNST LGNKQIGVEN
    1601  LQTLSPLILR TIPVSHKMDG LQDKVLELSL GFTKLD*

external links for Mon2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Mon2p

1)	Jochum A, et al. (2002) Yeast Ysl2p, homologous to Sec7 domain guanine nucleotide exchange factors, functions in endocytosis and maintenance of vacuole integrity and interacts with the Arf-Like small GTPase Arl1p. Mol Cell Biol 22(13):4914-28
2)	Muren E, et al. (2001) Identification of yeast deletion strains that are hypersensitive to brefeldin A or monensin, two drugs that affect intracellular transport. Yeast 18(2):163-72
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41