GIS2/YNL255C Protein Information Help

Standard Name Gis2p 1
Systematic Name Ynl255cp
ORF Classification Verified
Description Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 (2, 3, 4, 5)
Name Description GIg Suppressor 1
Experimental Data
Molecules/cell 96700 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 153
Molecular Weight (Da) 17,103
Isoelectric Point (pI) 7.71

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Gis2p (InterPro)
Physical Interactions There are 1319 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013BBDD | P53849
MIPS: YNL255C
NCBI: 1255963 | 1302303 | 1730843 | 45269894 | 6324074 | NP_014144.1
GenBank/EMBL/DDBJ: DAA10304.1 | AY558002 | X96722 | Z71531
Amino Acid Sequence (or in FASTA format)
 
       1  MSQKACYVCG KIGHLAEDCD SERLCYNCNK PGHVQTDCTM PRTVEFKQCY
      51  NCGETGHVRS ECTVQRCFNC NQTGHISREC PEPKKTSRFS KVSCYKCGGP
     101  NHMAKDCMKE DGISGLKCYT CGQAGHMSRD CQNDRLCYNC NETGHISKDC
     151  PKA*

external links for Gis2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Gis2p
1) Balciunas D and Ronne H  (1999) Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells. Mol Gen Genet 262(4-5):589-99
2) Tsvetanova NG, et al.  (2010) Proteome-Wide Search Reveals Unexpected RNA-Binding Proteins in Saccharomyces cerevisiae.LID - e12671 [pii] PLoS One 5(9)
3) Scherrer T, et al.  (2011) Defining potentially conserved RNA regulons of homologous zinc-finger RNA-binding proteins. Genome Biol 12(1):R3
4) Sammons MA, et al.  (2011) Saccharomyces cerevisiae Gis2 interacts with the translation machinery and is orthogonal to myotonic dystrophy type 2 protein ZNF9. Biochem Biophys Res Commun 406(1):13-9
5) Rojas M, et al.  (2012) Yeast Gis2 and Its Human Ortholog CNBP Are Novel Components of Stress-Induced RNP Granules. PLoS One 7(12):e52824
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41