NRD1/YNL251C Protein Information Help

Standard Name Nrd1p 1
Systematic Name Ynl251cp
ORF Classification Verified
Description RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites (2, 3, 4, 5, 6)
Name Description Nuclear pre-mRNA Down-regulation 1
Experimental Data
Molecules/cell 19600 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 575
Molecular Weight (Da) 63,858
Isoelectric Point (pI) 9.96

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Nrd1p (InterPro)
Physical Interactions There are 160 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052FB7 | P53617
MIPS: YNL251C
NCBI: 1255967 | 1302295 | 1709346 | 194709166 | 433286558 | 6324078 | 887885 | NP_014148.1
GenBank/EMBL/DDBJ: DAA10308.1 | U28487 | X96722 | Z71527
Amino Acid Sequence (or in FASTA format)
 
       1  MQQDDDFQNF VATLESFKDL KSGISGSRIK KLTTYALDHI DIESKIISLI
      51  IDYSRLCPDS HKLGSLYIID SIGRAYLDET RSNSNSSSNK PGTCAHAINT
     101  LGEVIQELLS DAIAKSNQDH KEKIRMLLDI WDRSGLFQKS YLNAIRSKCF
     151  AMDISNNTAN TASQQLSLDP KQRSKQILSN LKKSPPLNLN ISLPTDLTST
     201  DPAKQQAALF QVIAALQKHF KTLPSHTSVG TVAPPQAHTI TEYGSRRERE
     251  RERERYNSRR NRSRSPPAPF SQPSTGRKDR YPSVAQDQYS IGAPNTTFGT
     301  NNHHLYPDEL NVSNNPHYRP KPVSYDSTLP PDHIKVYSRT LFIGGVPLNM
     351  KEWDLANVLK PFAEVQSVIL NNSRKHAFVK VYSRHEAENV LQNFNKDGAL
     401  PLRTRWGVGF GPRDCCDYQH GYSIIPMHRL TDADKKWSVS AQWGGTSGQP
     451  LVTGIVFEEP DIIVGEGVSS KAISQKMPTD SGRNGPRSGK PNKSGSISSI
     501  SPVPYGNAPL ASPPPQQYVQ PMMQQPYGYA PNQPLPSQGP AAAAPPVPQQ
     551  QFDPTAQLNS LMNMLNQQQQ QQQQS*

external links for Nrd1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Nrd1p
1) Steinmetz EJ and Brow DA  (1996) Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1. Mol Cell Biol 16(12):6993-7003
2) Conrad NK, et al.  (2000) A yeast heterogeneous nuclear ribonucleoprotein complex associated with RNA polymerase II. Genetics 154(2):557-71
3) Steinmetz EJ and Brow DA  (1998) Control of pre-mRNA accumulation by the essential yeast protein Nrd1 requires high-affinity transcript binding and a domain implicated in RNA polymerase II association. Proc Natl Acad Sci U S A 95(12):6699-704
4) Steinmetz EJ, et al.  (2001) RNA-binding protein Nrd1 directs poly(A)-independent 3'-end formation of RNA polymerase II transcripts. Nature 413(6853):327-31
5) Vasiljeva L, et al.  (2008) The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. Nat Struct Mol Biol 15(8):795-804
6) Terzi N, et al.  (2011) H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway. Mol Cell Biol 31(17):3569-83
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41