RAD50/YNL250W Protein Information Help

Standard Name Rad50p 1
Systematic Name Ynl250wp
ORF Classification Verified
Description Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress (2, 3)
Name Description RADiation sensitive
Experimental Data
Molecules/cell 830 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,312
Molecular Weight (Da) 152,568
Isoelectric Point (pI) 5.92

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rad50p (InterPro)
Physical Interactions There are 73 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000004C4DD | P12753
MIPS: YNL250W
NCBI: 1255968 | 1302293 | 131782 | 4273 | 6324079 | NP_014149.1
GenBank/EMBL/DDBJ: DAA10309.1 | X14814 | X96722 | Z71526
External Classifications EC: 3.6.-.- [Hydrolases, Acting on Acid Anhydrides]
Amino Acid Sequence (or in FASTA format)
 
       1  MSAIYKLSIQ GIRSFDSNDR ETIEFGKPLT LIVGMNGSGK TTIIECLKYA
      51  TTGDLPPNSK GGVFIHDPKI TGEKDIRAQV KLAFTSANGL NMIVTRNIQL
     101  LMKKTTTTFK TLEGQLVAIN NSGDRSTLST RSLELDAQVP LYLGVPKAIL
     151  EYVIFCHQED SLWPLSEPSN LKKKFDEIFQ AMKFTKALDN LKSIKKDMSV
     201  DIKLLKQSVE HLKLDKDRSK AMKLNIHQLQ TKIDQYNEEV SEIESQLNEI
     251  TEKSDKLFKS NQDFQKILSK VENLKNTKLS ISDQVKRLSN SIDILDLSKP
     301  DLQNLLANFS KVLMDKNNQL RDLETDISSL KDRQSSLQSL SNSLIRRQGE
     351  LEAGKETYEK NRNHLSSLKE AFQHKFQGLS NIENSDMAQV NHEMSQFKAF
     401  ISQDLTDTID QFAKDIQLKE TNLSDLIKSI TVDSQNLEYN KKDRSKLIHD
     451  SEELAEKLKS FKSLSTQDSL NHELENLKTY KEKLQSWESE NIIPKLNQKI
     501  EEKNNEMIIL ENQIEKFQDR IMKTNQQADL YAKLGLIKKS INTKLDELQK
     551  ITEKLQNDSR IRQVFPLTQE FQRADLEMDF QKLFINMQKN IAINNKKMHE
     601  LDRRYTNALY NLNTIEKDLQ DNQKSKEKVI QLLSENLPED CTIDEYNDVL
     651  EETELSYKTA LENLKMHQTT LEFNRKALEI AERDSCCYLC SRKFENESFK
     701  SKLLQELKTK TDANFEKTLK DTVQNEKEYL HSLRLLEKHI ITLNSINEKI
     751  DNSQKCLEKA KEETKTSKSK LDELEVDSTK LKDEKELAES EIRPLIEKFT
     801  YLEKELKDLE NSSKTISEEL SIYNTSEDGI QTVDELRDQQ RKMNDSLREL
     851  RKTISDLQME KDEKVRENSR MINLIKEKEL TVSEIESSLT QKQNIDDSIR
     901  SKRENINDID SRVKELEARI ISLKNKKDEA QSVLDKVKNE RDIQVRNKQK
     951  TVADINRLID RFQTIYNEVV DFEAKGFDEL QTTIKELELN KAQMLELKEQ
    1001  LDLKSNEVNE EKRKLADSNN EEKNLKQNLE LIELKSQLQH IESEISRLDV
    1051  QNAEAERDKY QEESLRLRTR FEKLSSENAG KLGEMKQLQN QIDSLTHQLR
    1101  TDYKDIEKNY HKEWVELQTR SFVTDDIDVY SKALDSAIMK YHGLKMQDIN
    1151  RIIDELWKRT YSGTDIDTIK IRSDEVSSTV KGKSYNYRVV MYKQDVELDM
    1201  RGRCSAGQKV LASIIIRLAL SETFGANCGV IALDEPTTNL DEENIESLAK
    1251  SLHNIINMRR HQKNFQLIVI THDEKFLGHM NAAAFTDHFF KVKRDDRQKS
    1301  QIEWVDINRV TY*

external links for Rad50p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rad50p
1) Game, J.  (1985) Personal Communication, Mortimer Map Edition 9
2) Symington LS  (2002) Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol Mol Biol Rev 66(4):630-70, table of contents
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41