SLA2/YNL243W Protein Information

Standard Name	Sla2p 1
Systematic Name	Ynl243wp
Alias	End4p 2, 3 , Mop2p 4
ORF Classification	Verified
Description	Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo (5, 6, 7, 8, 9)
Name Description	Synthetic Lethal with ABP1 1

Experimental Data
Molecules/cell	40600 10

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	968
Molecular Weight (Da)	108,911
Isoelectric Point (pI)	5.11

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sla2p (InterPro)
Physical Interactions	There are 77 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001359FD \| P33338 MIPS: YNL243W NCBI: 1183971 \| 1302280 \| 2253184 \| 2506215 \| 311413 \| 330443694 \| 347595827 \| 501048 \| 870732 \| NP_014156.2 GenBank/EMBL/DDBJ: DAA10316.2 \| L12352 \| U07938 \| Z22811 \| Z69381 \| Z71518 \| Z71519

Amino Acid Sequence (or in FASTA format)
1 MSRIDSDLQK ALKKACSVEE TAPKRKHVRA CIVYTWDHQS SKAVFTTLKT 51 LPLANDEVQL FKMLIVLHKI IQEGHPSALA EAIRDRDWIR SLGRVHSGGS 101 SYSKLIREYV RYLVLKLDFH AHHRGFNNGT FEYEEYVSLV SVSDPDEGYE 151 TILDLMSLQD SLDEFSQIIF ASIQSERRNT ECKISALIPL IAESYGIYKF 201 ITSMLRAMHR QLNDAEGDAA LQPLKERYEL QHARLFEFYA DCSSVKYLTT 251 LVTIPKLPVD APDVFLINDV DESKEIKFKK REPSVTPART PARTPTPTPP 301 VVAEPAISPR PVSQRTTSTP TGYLQTMPTG ATTGMMIPTA TGAANAIFPQ 351 ATAQMQPDFW ANQQAQFANE QNRLEQERVQ QLQQQQAQQE LFQQQLQKAQ 401 QDMMNMQLQQ QNQHQNDLIA LTNQYEKDQA LLQQYDQRVQ QLESEITTMD 451 STASKQLANK DEQLTALQDQ LDVWERKYES LAKLYSQLRQ EHLNLLPRFK 501 KLQLKVNSAQ ESIQKKEQLE HKLKQKDLQM AELVKDRDRA RLELERSINN 551 AEADSAAATA AAETMTQDKM NPILDAILES GINTIQESVY NLDSPLSWSG 601 PLTPPTFLLS LLESTSENAT EFATSFNNLI VDGLAHGDQT EVIHCVSDFS 651 TSMATLVTNS KAYAVTTLPQ EQSDQILTLV KRCAREAQYF FEDLMSENLN 701 QVGDEEKTDI VINANVDMQE KLQELSLAIE PLLNIQSVKS NKETNPHSEL 751 VATADKIVKS SEHLRVDVPK PLLSLALMII DAVVALVKAA IQCQNEIATT 801 TSIPLNQFYL KNSRWTEGLI SAAKAVAGAT NVLITTASKL ITSEDNENTS 851 PEQFIVASKE VAASTIQLVA ASRVKTSIHS KAQDKLEHCS KDVTDACRSL 901 GNHVMGMIED DHSTSQQQQP LDFTSEHTLK TAEMEQQVEI LKLEQSLSNA 951 RKRLGEIRRH AYYNQDDD*

Amino Acid Sequence (or in FASTA format)

       1  MSRIDSDLQK ALKKACSVEE TAPKRKHVRA CIVYTWDHQS SKAVFTTLKT
      51  LPLANDEVQL FKMLIVLHKI IQEGHPSALA EAIRDRDWIR SLGRVHSGGS
     101  SYSKLIREYV RYLVLKLDFH AHHRGFNNGT FEYEEYVSLV SVSDPDEGYE
     151  TILDLMSLQD SLDEFSQIIF ASIQSERRNT ECKISALIPL IAESYGIYKF
     201  ITSMLRAMHR QLNDAEGDAA LQPLKERYEL QHARLFEFYA DCSSVKYLTT
     251  LVTIPKLPVD APDVFLINDV DESKEIKFKK REPSVTPART PARTPTPTPP
     301  VVAEPAISPR PVSQRTTSTP TGYLQTMPTG ATTGMMIPTA TGAANAIFPQ
     351  ATAQMQPDFW ANQQAQFANE QNRLEQERVQ QLQQQQAQQE LFQQQLQKAQ
     401  QDMMNMQLQQ QNQHQNDLIA LTNQYEKDQA LLQQYDQRVQ QLESEITTMD
     451  STASKQLANK DEQLTALQDQ LDVWERKYES LAKLYSQLRQ EHLNLLPRFK
     501  KLQLKVNSAQ ESIQKKEQLE HKLKQKDLQM AELVKDRDRA RLELERSINN
     551  AEADSAAATA AAETMTQDKM NPILDAILES GINTIQESVY NLDSPLSWSG
     601  PLTPPTFLLS LLESTSENAT EFATSFNNLI VDGLAHGDQT EVIHCVSDFS
     651  TSMATLVTNS KAYAVTTLPQ EQSDQILTLV KRCAREAQYF FEDLMSENLN
     701  QVGDEEKTDI VINANVDMQE KLQELSLAIE PLLNIQSVKS NKETNPHSEL
     751  VATADKIVKS SEHLRVDVPK PLLSLALMII DAVVALVKAA IQCQNEIATT
     801  TSIPLNQFYL KNSRWTEGLI SAAKAVAGAT NVLITTASKL ITSEDNENTS
     851  PEQFIVASKE VAASTIQLVA ASRVKTSIHS KAQDKLEHCS KDVTDACRSL
     901  GNHVMGMIED DHSTSQQQQP LDFTSEHTLK TAEMEQQVEI LKLEQSLSNA
     951  RKRLGEIRRH AYYNQDDD*

external links for Sla2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Sla2p

1)	Holtzman DA, et al. (1993) Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. J Cell Biol 122(3):635-44
2)	Wesp A, et al. (1997) End4p/Sla2p interacts with actin-associated proteins for endocytosis in Saccharomyces cerevisiae. Mol Biol Cell 8(11):2291-306
3)	Raths S, et al. (1993) end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae. J Cell Biol 120(1):55-65
4)	Na S, et al. (1995) MOP2 (SLA2) affects the abundance of the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae. J Biol Chem 270(12):6815-23
5)	Pruyne D and Bretscher A (2000) Polarization of cell growth in yeast. J Cell Sci 113 ( Pt 4):571-85
6)	Yang S, et al. (1999) Sla2p is associated with the yeast cortical actin cytoskeleton via redundant localization signals. Mol Biol Cell 10(7):2265-83
7)	Munn AL (2001) Molecular requirements for the internalisation step of endocytosis: insights from yeast. Biochim Biophys Acta 1535(3):236-57
8)	Palecek J, et al. (2001) Rpg1p/Tif32p, a subunit of translation initiation factor 3, interacts with actin-associated protein Sla2p. Biochem Biophys Res Commun 282(5):1244-50
9)	Baggett JJ, et al. (2003) The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae. Genetics 165(4):1661-74
10)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41