SIN4/YNL236W Protein Information Help

Standard Name Sin4p 1, 2
Systematic Name Ynl236wp
Alias Bel2p , Gal22p , Sdi3p 3 , Ssf5p , Ssn4p , Tsf3p 3 , Rye1p , Med16p 4
ORF Classification Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription (5, 6)
Name Description Switch INdependent
Experimental Data
Molecules/cell 1720 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 974
Molecular Weight (Da) 111,296
Isoelectric Point (pI) 8.78

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Sin4p (InterPro)
Physical Interactions There are 123 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000005315A | P32259
MIPS: YNL236W
NCBI: 1183978 | 1302265 | 172602 | 218406 | 417773 | 4681 | 6324093 | NP_014163.1
GenBank/EMBL/DDBJ: DAA10323.1 | D12918 | M93050 | X64516 | Z69381 | Z71512
Amino Acid Sequence (or in FASTA format)
 
       1  MMLGEHLMSW SKTGIIAYSD SQSSNANICL TFLESINGIN WRFHTPQKYV
      51  LHPQLHEVQY QESSSTLSTH STTTSVNGST TAGVGSTPNF GGNSNKSPPQ
     101  FFYNISSIHW NNWFSLPGDM LAVCDELGNM TMLITGQRPD RATTYEKLTM
     151  VFQDNVYKIY NHVMPLKPVD KLKPMNIERK QTRKEYNTSI LEFRWLTSSK
     201  SVIVSQFCAF DSSSNTYRSR AQQVPPYGVY HPPFIKYACL AIRKNGQIDF
     251  WYQFSNSKDH KKITLQLLDT SNQRFKDLQW LEFARITPMN DDQCMLITTY
     301  SKLSKNISFY KLHVNWNLNA TKPNVLNDPS LKIQFILSTT LDPTDDEGHV
     351  LKLENLHVVS KSSIEKDPSP EILVLYNVCD TSKSLVKRYR LAPTQLSAEY
     401  LVILKPDLNI DRNNSTNQIF QSRRYNLRRH SDIVLDKKVT LITSEMFDAF
     451  VSFYFEDGTI ESYNQNDWKL ETERLISQSQ LGKFKNIIAS PLSAGFNYGK
     501  LPLPPSVEWM KVSPSMCGVI VKQYNKKWPQ FYAAVQKNYA DPEKDSINAT
     551  ALAFGYVKSL HKQISAEDLT IAAKTHILRI SFLDRKRAKE FITTLLKSLY
     601  SFFNISPDAP KEIMDKIITS RPLQKIMLLQ LELGSCFSQE NIEEMARVIL
     651  YLKNVLFAFN GVARNFHFAI EQISNNSNQQ QNPKLFQTIF SKQDLIHSLI
     701  PVAKWFVKFI TYLTQEILIL INDPTNKEYT LVHGIFGAKM SRTLILSILN
     751  EIKKVTQIVA KFPETSYPIL NESSTFLKLV LSESPVDFEK FETFLVDVNN
     801  KFIALCEQQP SQEREFSLLV KAEIPPEYAK VGDFLLQYAN NAVISHANAA
     851  AVYFADTSGL KISNSEFFNP EIFHLLQPLE EGLIIDTDKL PIKNRTSKSF
     901  SKLLYDDVTC DKLSVSEISD GKLKRCSRCG SVTRAGNIIS SDKTIVPTSI
     951  QTKRWPTMYT RLCICSGMLF EMDG*

external links for Sin4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Sin4p
1) Jiang YW and Stillman DJ  (1992) Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae. Mol Cell Biol 12(10):4503-14
2) Chen, S.  (1992) Personal Communication, Mortimer Map Edition 11
3) Stillman DJ, et al.  (1994) Epistasis analysis of suppressor mutations that allow HO expression in the absence of the yeast SW15 transcriptional activator. Genetics 136(3):781-8
4) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
5) Li Y, et al.  (1995) Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme. Proc Natl Acad Sci U S A 92(24):10864-8
6) Myers LC and Kornberg RD  (2000) Mediator of transcriptional regulation. Annu Rev Biochem 69():729-49
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41