URE2/YNL229C Protein Information

Standard Name	Ure2p
Systematic Name	Ynl229cp
ORF Classification	Verified
Description	Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion (1, 2, 3, 4, 5) Also known as: [URE3] 3
Name Description	UREidosuccinate transport

Experimental Data
Molecules/cell	7060 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	354
Molecular Weight (Da)	40,271
Isoelectric Point (pI)	6.45

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ure2p (InterPro)
Physical Interactions	There are 81 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000003993 \| P23202 MIPS: YNL229C NCBI: 1183985 \| 1302253 \| 13399769 \| 13399770 \| 13399771 \| 13399772 \| 13399773 \| 13399774 \| 13399967 \| 13399968 \| 137083 \| 173152 \| 18158856 \| 18158857 \| 18158858 \| 18158859 \| 18158861 \| 18158862 \| 18158863 \| 18158864 \| 18158865 \| 18158866 \| 18158867 \| 18158868 \| 18158869 \| 18158870 \| 18158871 \| 18158872 \| 18158873 \| 18158874 \| 22135373 \| 22135375 \| 22135377 \| 22135379 \| 22135381 \| 22135383 \| 22135385 \| 22135387 \| 22135389 \| 22135391 \| 22135393 \| 22135395 \| 22135397 \| 22135399 \| 22135401 \| 22135403 \| 22135405 \| 22135407 \| 22135409 \| 6324100 \| NP_014170.1 GenBank/EMBL/DDBJ: DAA10329.1 \| AF525174 \| AF525175 \| AF525176 \| AF525177 \| AF525178 \| AF525179 \| AF525180 \| AF525181 \| AF525182 \| AF525183 \| AF525184 \| AF525185 \| AF525186 \| AF525187 \| AF525188 \| AF525189 \| AF525190 \| AF525191 \| AF525192 \| M35268 \| Z69381 \| Z71505

Amino Acid Sequence (or in FASTA format)
1 MMNNNGNQVS NLSNALRQVN IGNRNSNTTT DQSNINFEFS TGVNNNNNNN 51 SSSNNNNVQN NNSGRNGSQN NDNENNIKNT LEQHRQQQQA FSDMSHVEYS 101 RITKFFQEQP LEGYTLFSHR SAPNGFKVAI VLSELGFHYN TIFLDFNLGE 151 HRAPEFVSVN PNARVPALID HGMDNLSIWE SGAILLHLVN KYYKETGNPL 201 LWSDDLADQS QINAWLFFQT SGHAPMIGQA LHFRYFHSQK IASAVERYTD 251 EVRRVYGVVE MALAERREAL VMELDTENAA AYSAGTTPMS QSRFFDYPVW 301 LVGDKLTIAD LAFVPWNNVV DRIGINIKIE FPEVYKWTKH MMRRPAVIKA 351 LRGE*

Amino Acid Sequence (or in FASTA format)

       1  MMNNNGNQVS NLSNALRQVN IGNRNSNTTT DQSNINFEFS TGVNNNNNNN
      51  SSSNNNNVQN NNSGRNGSQN NDNENNIKNT LEQHRQQQQA FSDMSHVEYS
     101  RITKFFQEQP LEGYTLFSHR SAPNGFKVAI VLSELGFHYN TIFLDFNLGE
     151  HRAPEFVSVN PNARVPALID HGMDNLSIWE SGAILLHLVN KYYKETGNPL
     201  LWSDDLADQS QINAWLFFQT SGHAPMIGQA LHFRYFHSQK IASAVERYTD
     251  EVRRVYGVVE MALAERREAL VMELDTENAA AYSAGTTPMS QSRFFDYPVW
     301  LVGDKLTIAD LAFVPWNNVV DRIGINIKIE FPEVYKWTKH MMRRPAVIKA
     351  LRGE*

external links for Ure2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ure2p

1)	Courchesne WE and Magasanik B (1988) Regulation of nitrogen assimilation in Saccharomyces cerevisiae: roles of the URE2 and GLN3 genes. J Bacteriol 170(2):708-13
2)	Blinder D, et al. (1996) Interaction of the GATA factor Gln3p with the nitrogen regulator Ure2p in Saccharomyces cerevisiae. J Bacteriol 178(15):4734-6
3)	Wickner RB (1994) [URE3] as an altered URE2 protein: evidence for a prion analog in Saccharomyces cerevisiae. Science 264(5158):566-9
4)	Bai M, et al. (2004) The yeast prion protein Ure2 shows glutathione peroxidase activity in both native and fibrillar forms. J Biol Chem 279(48):50025-30
5)	Zhang ZR, et al. (2008) "Restoration" of glutathione transferase activity by single-site mutation of the yeast prion protein Ure2. J Mol Biol 384(3):641-51
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41