JJJ1/YNL227C Protein Information

Standard Name	Jjj1p 1
Systematic Name	Ynl227cp
ORF Classification	Verified
Description	Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis (1, 2, 3)
Name Description	J-protein (Type III) 1

Experimental Data
Molecules/cell	2310 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	590
Molecular Weight (Da)	68,782
Isoelectric Point (pI)	6.14

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Jjj1p (InterPro)
Physical Interactions	There are 32 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013BBCC \| P53863 MIPS: YNL227C NCBI: 1183987 \| 1302250 \| 1730828 \| 6324103 \| NP_014172.1 GenBank/EMBL/DDBJ: DAA10330.1 \| Z69381 \| Z71504

Amino Acid Sequence (or in FASTA format)
1 MKTCYYELLG VETHASDLEL KKAYRKKALQ YHPDKNPDNV EEATQKFAVI 51 RAAYEVLSDP QERAWYDSHK EQILNDTPPS TDDYYDYEVD ATVTGVTTDE 101 LLLFFNSALY TKIDNSAAGI YQIAGKIFAK LAKDEILSGK RLGKFSEYQD 151 DVFEQDINSI GYLKACDNFI NKTDKLLYPL FGYSPTDYEY LKHFYKTWSA 201 FNTLKSFSWK DEYMYSKNYD RRTKREVNRR NEKARQQARN EYNKTVKRFV 251 VFIKKLDKRM KEGAKIAEEQ RKLKEQQRKN ELNNRRKFGN DNNDEEKFHL 301 QSWQTVKEEN WDELEKVYDN FGEFENSKND KEGEVLIYEC FICNKTFKSE 351 KQLKNHINTK LHKKNMEEIR KEMEEENITL GLDNLSDLEK FDSADESVKE 401 KEDIDLQALQ AELAEIERKL AESSSEDESE DDNLNIEMDI EVEDVSSDEN 451 VHVNTKNKKK RKKKKKAKVD TETEESESFD DTKDKRSNEL DDLLASLGDK 501 GLQTDDDEDW STKAKKKKGK QPKKNSKSTK STPSLSTLPS SMSPTSAIEV 551 CTTCGESFDS RNKLFNHVKI AGHAAVKNVV KRKKVKTKRI *

Amino Acid Sequence (or in FASTA format)

       1  MKTCYYELLG VETHASDLEL KKAYRKKALQ YHPDKNPDNV EEATQKFAVI
      51  RAAYEVLSDP QERAWYDSHK EQILNDTPPS TDDYYDYEVD ATVTGVTTDE
     101  LLLFFNSALY TKIDNSAAGI YQIAGKIFAK LAKDEILSGK RLGKFSEYQD
     151  DVFEQDINSI GYLKACDNFI NKTDKLLYPL FGYSPTDYEY LKHFYKTWSA
     201  FNTLKSFSWK DEYMYSKNYD RRTKREVNRR NEKARQQARN EYNKTVKRFV
     251  VFIKKLDKRM KEGAKIAEEQ RKLKEQQRKN ELNNRRKFGN DNNDEEKFHL
     301  QSWQTVKEEN WDELEKVYDN FGEFENSKND KEGEVLIYEC FICNKTFKSE
     351  KQLKNHINTK LHKKNMEEIR KEMEEENITL GLDNLSDLEK FDSADESVKE
     401  KEDIDLQALQ AELAEIERKL AESSSEDESE DDNLNIEMDI EVEDVSSDEN
     451  VHVNTKNKKK RKKKKKAKVD TETEESESFD DTKDKRSNEL DDLLASLGDK
     501  GLQTDDDEDW STKAKKKKGK QPKKNSKSTK STPSLSTLPS SMSPTSAIEV
     551  CTTCGESFDS RNKLFNHVKI AGHAAVKNVV KRKKVKTKRI *

external links for Jjj1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Jjj1p

1)	Walsh P, et al. (2004) The J-protein family: modulating protein assembly, disassembly and translocation. EMBO Rep 5(6):567-71
2)	Wiederkehr A, et al. (2001) Identification and characterization of Saccharomyces cerevisiae mutants defective in fluid-phase endocytosis. Yeast 18(8):759-73
3)	Meyer AE, et al. (2007) The specialized cytosolic J-protein, Jjj1, functions in 60S ribosomal subunit biogenesis. Proc Natl Acad Sci U S A 104(5):1558-63
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41