ALG9/YNL219C Protein Information

Standard Name	Alg9p 1
Systematic Name	Ynl219cp
ORF Classification	Verified
Description	Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation (1, 2, 3)
Name Description	Asparagine-Linked Glycosylation 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	555
Molecular Weight (Da)	63,776
Isoelectric Point (pI)	8.74

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Alg9p (InterPro)
Transmembrane Domains	There are 7 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 9 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000125846 \| P53868 MIPS: YNL219C NCBI: 1302235 \| 1552381 \| 1730823 \| 6324110 \| NP_014180.1 GenBank/EMBL/DDBJ: DAA10337.1 \| X96417 \| Z71495

Amino Acid Sequence (or in FASTA format)
1 MNCKAVTISL LLLLFLTRVY IQPTFSLISD CDETFNYWEP LNLLVRGFGK 51 QTWEYSPEYS IRSWAFLLPF YCILYPVNKF TDLESHWNFF ITRACLGFFS 101 FIMEFKLHRE IAGSLALQIA NIWIIFQLFN PGWFHASVEL LPSAVAMLLY 151 VGATRHSLRY LSTGSTSNFT KSLAYNFLAS ILGWPFVLIL SLPLCLHYLF 201 NHRIISTIRT AFDCCLIFSL TAFAVIVTDS IFYGKLAPVS WNILFYNVIN 251 ASEESGPNIF GVEPWYYYPL NLLLNFPLPV LVLAILGIFH LRLWPLWASL 301 FTWIAVFTQQ PHKEERFLYP IYGLITLSAS IAFYKVLNLF NRKPILKKGI 351 KLSVLLIVAG QAMSRIVALV NNYTAPIAVY EQFSSLNQGG VKAPVVNVCT 401 GREWYHFPSS FLLPDNHRLK FVKSGFDGLL PGDFPESGSI FKKIRTLPKG 451 MNNKNIYDTG KEWPITRCDY FIDIVAPINL TKDVFNPLHL MDNWNKLACA 501 AFIDGENSKI LGRAFYVPEP INRIMQIVLP KQWNQVYGVR YIDYCLFEKP 551 TETTN*

Amino Acid Sequence (or in FASTA format)

       1  MNCKAVTISL LLLLFLTRVY IQPTFSLISD CDETFNYWEP LNLLVRGFGK
      51  QTWEYSPEYS IRSWAFLLPF YCILYPVNKF TDLESHWNFF ITRACLGFFS
     101  FIMEFKLHRE IAGSLALQIA NIWIIFQLFN PGWFHASVEL LPSAVAMLLY
     151  VGATRHSLRY LSTGSTSNFT KSLAYNFLAS ILGWPFVLIL SLPLCLHYLF
     201  NHRIISTIRT AFDCCLIFSL TAFAVIVTDS IFYGKLAPVS WNILFYNVIN
     251  ASEESGPNIF GVEPWYYYPL NLLLNFPLPV LVLAILGIFH LRLWPLWASL
     301  FTWIAVFTQQ PHKEERFLYP IYGLITLSAS IAFYKVLNLF NRKPILKKGI
     351  KLSVLLIVAG QAMSRIVALV NNYTAPIAVY EQFSSLNQGG VKAPVVNVCT
     401  GREWYHFPSS FLLPDNHRLK FVKSGFDGLL PGDFPESGSI FKKIRTLPKG
     451  MNNKNIYDTG KEWPITRCDY FIDIVAPINL TKDVFNPLHL MDNWNKLACA
     501  AFIDGENSKI LGRAFYVPEP INRIMQIVLP KQWNQVYGVR YIDYCLFEKP
     551  TETTN*

external links for Alg9p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Alg9p

1)	Burda P, et al. (1996) Stepwise assembly of the lipid-linked oligosaccharide in the endoplasmic reticulum of Saccharomyces cerevisiae: identification of the ALG9 gene encoding a putative mannosyl transferase. Proc Natl Acad Sci U S A 93(14):7160-5
2)	Frank CG, et al. (2004) Identification and functional analysis of a defect in the human ALG9 gene: definition of congenital disorder of glycosylation type IL. Am J Hum Genet 75(1):146-50
3)	Frank CG and Aebi M (2005) ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis. Glycobiology 15(11):1156-63
4)	Huffaker TC and Robbins PW (1982) Temperature-sensitive yeast mutants deficient in asparagine-linked glycosylation. J Biol Chem 257(6):3203-10