RIO2/YNL207W Protein Information

Standard Name	Rio2p 1, 2
Systematic Name	Ynl207wp
ORF Classification	Verified
Description	Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p (1, 2)
Name Description	RIght Open reading frame 3

Experimental Data
Molecules/cell	10300 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	425
Molecular Weight (Da)	49,124
Isoelectric Point (pI)	5

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rio2p (InterPro)
Physical Interactions	There are 192 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FD6 \| P40160 MIPS: YNL207W NCBI: 1302211 \| 600058 \| 6324122 \| 732189 \| NP_014192.1 GenBank/EMBL/DDBJ: DAA10349.1 \| X78898 \| Z71483
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MKLDTSHMRY LTTDDFRVLQ AVEQGSRSHE VVPTPLIHQI SGMRSQSGTN 51 RAISDLAKLS LISKMRNVKY DGYRLTYNGI DYLALKTMLN RDTVYSVGNT 101 IGVGKESDIY KVSDKNGNPR VMKIHRLGRT SFHSVRNNRD YLKKSNQGAN 151 WMHLSRLAAN KEYQFMSMLY SKGFKVPEPF DNSRHIVVME LIEGYPMRRL 201 RKHKNIPKLY SDLMCFIVDL ANSGLIHCDF NEFNIMIKDK LEDENDCGFV 251 VIDFPQCISI QHQDADYYFQ RDVDCIRRFF KKKLKYEPKP DSSMLDTEGF 301 GDGYKYAYPD FKRDVKRTDN LDELVQASGF SKKHPGDRGL ETAVESMRNA 351 VYNSDDDMSN DEAEEENGEG DYSEEDEYYD SELDNESSED DSEDAQEEEN 401 ERIIEALSSG VENLKMDKLG NYILE*

Amino Acid Sequence (or in FASTA format)

       1  MKLDTSHMRY LTTDDFRVLQ AVEQGSRSHE VVPTPLIHQI SGMRSQSGTN
      51  RAISDLAKLS LISKMRNVKY DGYRLTYNGI DYLALKTMLN RDTVYSVGNT
     101  IGVGKESDIY KVSDKNGNPR VMKIHRLGRT SFHSVRNNRD YLKKSNQGAN
     151  WMHLSRLAAN KEYQFMSMLY SKGFKVPEPF DNSRHIVVME LIEGYPMRRL
     201  RKHKNIPKLY SDLMCFIVDL ANSGLIHCDF NEFNIMIKDK LEDENDCGFV
     251  VIDFPQCISI QHQDADYYFQ RDVDCIRRFF KKKLKYEPKP DSSMLDTEGF
     301  GDGYKYAYPD FKRDVKRTDN LDELVQASGF SKKHPGDRGL ETAVESMRNA
     351  VYNSDDDMSN DEAEEENGEG DYSEEDEYYD SELDNESSED DSEDAQEEEN
     401  ERIIEALSSG VENLKMDKLG NYILE*

external links for Rio2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rio2p

1)	Vanrobays E, et al. (2003) Late cytoplasmic maturation of the small ribosomal subunit requires RIO proteins in Saccharomyces cerevisiae. Mol Cell Biol 23(6):2083-95
2)	Geerlings TH, et al. (2003) Rio2p, an evolutionarily conserved, low abundant protein kinase essential for processing of 20 S Pre-rRNA in Saccharomyces cerevisiae. J Biol Chem 278(25):22537-45
3)	Angermayr M and Bandlow W (1997) The type of basal promoter determines the regulated or constitutive mode of transcription in the common control region of the yeast gene pair GCY1/RIO1. J Biol Chem 272(50):31630-5
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41