PSY2/YNL201C Protein Information

Standard Name	Psy2p
Systematic Name	Ynl201cp
ORF Classification	Verified
Description	Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 (1, 2, 3, 4)
Name Description	Platinum SensitivitY 5

Experimental Data
Molecules/cell	7010 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	858
Molecular Weight (Da)	98,052
Isoelectric Point (pI)	4.81

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Psy2p (InterPro)
Physical Interactions	There are 77 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053120 \| P40164 MIPS: YNL201C NCBI: 1302202 \| 600064 \| 6324128 \| 732193 \| NP_014198.1 GenBank/EMBL/DDBJ: DAA10353.1 \| X78898 \| Z71477

Amino Acid Sequence (or in FASTA format)
1 MSLPGTPTTS PTPMDEDTEQ AVSVNTEPKR VKVYILENNE WKDTGTGFCI 51 GEVDEGKFAY LVVSDEDSPT ETLLKSKLEG NIEYQRQEET LIVWKDLGGK 101 DIALSFEESM GCDTLCEFIV HVQRNIESNI SLVTVKSSDN GLGSVHDIIT 151 GPVTLPSNDQ QQNSQTLLEA LKILNENTSF DFLKNETIEF ILQSNYIDTL 201 ISHFHKAEEE KIPKDLFLLS NIIKTLILYN KRDILESMVE DDRIMGIVGI 251 LEYDTEYPTS KANHRKYLGS KGPNFKEVIP LENEDLKIIM KKCFRLQFLK 301 DVVLVRFLDD HNFNLISEIV MDLETCIIDF LQVGTFLDRL IELYDTKTLP 351 ESSSEKEKFV QKRKDGIRLL QQCVQMSINL DAVDRSKFYK TLVRKGLFKV 401 LDYAFHMETD SNVRILATDT IITIIEHDIL LIHNVQNEDS FKRQHKSAPD 451 DKSSHRKYPQ DYSSSTDSKL LLILSTILLS DRSPGLREQV VQALNTLLHP 501 EGCVGNGEGS YDLMGRSNYE AKNTSEDFPS FSYGLNSDSI NLNNYHYSSD 551 EMNNLEPESE SEFQVMEYFA NFYNKIAPIL FGPLIKKDIT TEMAEIDGQI 601 EKVTKDDLLL IHLVKLVSFV CTEHDRVLSR RFILENGILD SVSKLIGGNH 651 MMQLRLTAVR CIKNLMCLDD KYYHRYMISK NLYAPVFKLF QENIDKNNLA 701 NSCIQDFFRI IITECRAYQS DGHNRKEKTN GSYDGNGNDV KTNVNNNRTN 751 FTILNKYLVQ TYGDVLRKAT DIPFIQDMLE TGEENQPDHS SFENSIEGGN 801 DISVNMSTDG FASNHLEDID IKNVKRLHSE IEHFENDPHY SGDQLAFKKS 851 VDQMNAST*

Amino Acid Sequence (or in FASTA format)

       1  MSLPGTPTTS PTPMDEDTEQ AVSVNTEPKR VKVYILENNE WKDTGTGFCI
      51  GEVDEGKFAY LVVSDEDSPT ETLLKSKLEG NIEYQRQEET LIVWKDLGGK
     101  DIALSFEESM GCDTLCEFIV HVQRNIESNI SLVTVKSSDN GLGSVHDIIT
     151  GPVTLPSNDQ QQNSQTLLEA LKILNENTSF DFLKNETIEF ILQSNYIDTL
     201  ISHFHKAEEE KIPKDLFLLS NIIKTLILYN KRDILESMVE DDRIMGIVGI
     251  LEYDTEYPTS KANHRKYLGS KGPNFKEVIP LENEDLKIIM KKCFRLQFLK
     301  DVVLVRFLDD HNFNLISEIV MDLETCIIDF LQVGTFLDRL IELYDTKTLP
     351  ESSSEKEKFV QKRKDGIRLL QQCVQMSINL DAVDRSKFYK TLVRKGLFKV
     401  LDYAFHMETD SNVRILATDT IITIIEHDIL LIHNVQNEDS FKRQHKSAPD
     451  DKSSHRKYPQ DYSSSTDSKL LLILSTILLS DRSPGLREQV VQALNTLLHP
     501  EGCVGNGEGS YDLMGRSNYE AKNTSEDFPS FSYGLNSDSI NLNNYHYSSD
     551  EMNNLEPESE SEFQVMEYFA NFYNKIAPIL FGPLIKKDIT TEMAEIDGQI
     601  EKVTKDDLLL IHLVKLVSFV CTEHDRVLSR RFILENGILD SVSKLIGGNH
     651  MMQLRLTAVR CIKNLMCLDD KYYHRYMISK NLYAPVFKLF QENIDKNNLA
     701  NSCIQDFFRI IITECRAYQS DGHNRKEKTN GSYDGNGNDV KTNVNNNRTN
     751  FTILNKYLVQ TYGDVLRKAT DIPFIQDMLE TGEENQPDHS SFENSIEGGN
     801  DISVNMSTDG FASNHLEDID IKNVKRLHSE IEHFENDPHY SGDQLAFKKS
     851  VDQMNAST*

external links for Psy2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Psy2p

1)	Keogh MC, et al. (2006) A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature 439(7075):497-501
2)	Hastie CJ, et al. (2006) The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin. FEBS J 273(14):3322-34
3)	O'Neill BM, et al. (2007) Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage. Proc Natl Acad Sci U S A 104(22):9290-5
4)	Falk JE, et al. (2010) A Mec1- and PP4-dependent checkpoint couples centromere pairing to meiotic recombination. Dev Cell 19(4):599-611
5)	Wu HI, et al. (2004) Genome-wide identification of genes conferring resistance to the anticancer agents cisplatin, oxaliplatin, and mitomycin C. Cancer Res 64(11):3940-8
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41