IPI3/YNL182C Protein Information

Standard Name	Ipi3p 1
Systematic Name	Ynl182cp
ORF Classification	Verified
Description	Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene (1, 2, 3)
Name Description	Involved in Processing ITS2 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	555
Molecular Weight (Da)	61,772
Isoelectric Point (pI)	5.56

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ipi3p (InterPro)
Physical Interactions	There are 115 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000036C8E \| P53877 MIPS: YNL182C NCBI: 1302166 \| 1730812 \| 6324147 \| NP_014217.1 GenBank/EMBL/DDBJ: DAA10370.1 \| Z71458

Amino Acid Sequence (or in FASTA format)
1 MDEQVIFTTN TSGTIASVHS FEQINLRQCS TQSRNSCVQV GNKYLFIAQA 51 QKALINVYNL SGSFKRESVE QRLPLPEILK CLEVVENDGV QYDRIQGVNH 101 NLPDFNLPYL LLGSTESGKL YIWELNSGIL LNVKPMAHYQ SITKIKSILN 151 GKYIITSGND SRVIIWQTVD LVSASNDDPK PLCILHDHTL PVTDFQVSSS 201 QGKFLSCTDT KLFTVSQDAT IRCYDLSLIG SKKKQKANEN DVSIGKTPVL 251 LATFTTPYSI KSIVLDPADR ACYIGTAEGC FSLNLFYKLK GNAIVNLLQS 301 AGVNTVQKGR VFSLVQRNSL TGGENEDLDA LYAMGQLVCE NVLNSNVSCL 351 EISMDGTLLL IGDTEGKVSI AEIYSKQIIR TIQTLTTSQD SVGEVTNLLT 401 NPYRLERGNL LFEGESKGKQ PSNNNGHNFM KIPNLQRVIF DGKNKGHLHD 451 IWYQIGEPEA ETDPNLALPL NDFNAYLEQV KTQESIFSHI GKVSSNVKVI 501 DNKIDATSSL DSNAAKDEEI TELKTNIEAL THAYKELRDM HEKLYEEHQQ 551 MLDKQ*

Amino Acid Sequence (or in FASTA format)

       1  MDEQVIFTTN TSGTIASVHS FEQINLRQCS TQSRNSCVQV GNKYLFIAQA
      51  QKALINVYNL SGSFKRESVE QRLPLPEILK CLEVVENDGV QYDRIQGVNH
     101  NLPDFNLPYL LLGSTESGKL YIWELNSGIL LNVKPMAHYQ SITKIKSILN
     151  GKYIITSGND SRVIIWQTVD LVSASNDDPK PLCILHDHTL PVTDFQVSSS
     201  QGKFLSCTDT KLFTVSQDAT IRCYDLSLIG SKKKQKANEN DVSIGKTPVL
     251  LATFTTPYSI KSIVLDPADR ACYIGTAEGC FSLNLFYKLK GNAIVNLLQS
     301  AGVNTVQKGR VFSLVQRNSL TGGENEDLDA LYAMGQLVCE NVLNSNVSCL
     351  EISMDGTLLL IGDTEGKVSI AEIYSKQIIR TIQTLTTSQD SVGEVTNLLT
     401  NPYRLERGNL LFEGESKGKQ PSNNNGHNFM KIPNLQRVIF DGKNKGHLHD
     451  IWYQIGEPEA ETDPNLALPL NDFNAYLEQV KTQESIFSHI GKVSSNVKVI
     501  DNKIDATSSL DSNAAKDEEI TELKTNIEAL THAYKELRDM HEKLYEEHQQ
     551  MLDKQ*

external links for Ipi3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ipi3p

1)	Krogan NJ, et al. (2004) High-definition macromolecular composition of yeast RNA-processing complexes. Mol Cell 13(2):225-39
2)	Nissan TA, et al. (2004) A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits. Mol Cell 15(2):295-301
3)	Huo L, et al. (2012) The Rix1 (Ipi1p-2p-3p) complex is a critical determinant of DNA replication licensing independent of their roles in ribosome biogenesis. Cell Cycle 11(7):1325-39