APC1/YNL172W Protein Information

Standard Name	Apc1p 1
Systematic Name	Ynl172wp
ORF Classification	Verified
Description	Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress (1, 2, 3, 4, 5)
Name Description	Anaphase Promoting Complex subunit 1

Experimental Data
Molecules/cell	178 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,748
Molecular Weight (Da)	196,161
Isoelectric Point (pI)	5.8

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Apc1p (InterPro)
Physical Interactions	There are 90 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0001F7BD66 \| P53886 MIPS: YNL172W NCBI: 1302144 \| 1730803 \| 347595632 \| 398364519 \| NP_014227.4 GenBank/EMBL/DDBJ: DAA10378.2 \| Z71448

Amino Acid Sequence (or in FASTA format)
1 MTSKPSTRND YLPRETHNGE YTGDSPEWQL QINITNKIGG INGDIWLSRD 51 GRSVKWCIED QCLRQFTYNQ KIIKAGYIDF EKTPDCFVVV LSDIAHVYML 101 KNGGSTTVCF PFQIGNAFWY ANGVILERET SASFMDGGYD LKPIEFDLKH 151 KYITLTDPMA PFGLISITNT FKGGINSASG NKTDILQDFQ LVLFPSDKDK 201 CIAVFLDRNS KVLRFYYSRI LSSDQSRKGE LTISSTKKTG LDAAGNSQKS 251 GGISKDLRKF SHLTRRSTSI NSNSHDFNAA ERVLSGNVGN ASGRTDIFAL 301 PSSCSRRSLS ATLDRMGNNI APTNRAAPSG FFDSSSANTA THSNITPVSQ 351 PMQQQQQEYL NQTATSSKDI VLTEISSLKL PDDIIFTSRR LSSILSKLKF 401 LSLRFERREG LLIFHEPTHF CKIWLIDLLP DVLDSIPFKI YGNSPQNMIR 451 LENLKLKEPS RIQAMYIHEL LESCLILVSE GQNKEEYKAC LYDPFVKITS 501 PSKNISEELT KQNSLPSLQK LFPYPETSFT KLCFEAVKYI TSPAFNISFI 551 FLWQSAYSIL LSRANDDVVG GLKMEHDAFS LVLSLLILPI PSSSAQEYQE 601 YKEIYERDLF QHLKQDSEIT SSVLPRIVIG LHLIREEYSL NVLCRNEHAL 651 LGQFLRFATA AMGWPDLWQS YYVPKMDSES KTFLHPREQN STFFHPLDEP 701 PSITKSLYSI TENSSIPLCP FISFSRLVAT DTQVELRITP RSFKILGLYE 751 LVHSPNFLPD YVLGILSSFK VDKDELQTYP LGILVPLQNI LKILEDKLSE 801 VRDNLELLDR ADLQRCSAII NSIRSDSKEV LKRGQRDSYM LCKVPLAKNR 851 SSLSKKPSDI YSILSEIVKS ASQVPLDGSA MRMSNIQDDE DIDEGRSLKL 901 NAGLIFSEDK RFTHVVSLLA YYRPTKTQFF TTKTEYAQIL AQKKYFAKIM 951 ALRTCTNGVG WGAVAYATEK PISTQKWVIQ PLNLISVFPD DTKITVKAPE 1001 DIAHDIVEWG QFHAGVSSGL RISKKATGIT GSWIAFNKPK ELDAYHGGFL 1051 LGLGLNGHLK NLEEWHIYNY LSPRNTHISI GLLLGMSSSM KGSMDSKLIK 1101 VISVHLVAFL PSGSSDLNID LKLQTAGIIG MGMLYLNSRH KRMSDSIFAQ 1151 LVSLLNVNDE MVADEEYRLA AGISLGLINL GAGQTKLRKW DSSLLGLGDD 1201 LPEDVYDSSD VEQNVMYEDL TTKLLEIVTS TYDVENDWIP ENSQIGAVIA 1251 IMFLFLKSNN FGISNMLKVD LKEILKANIN TRPELLMYRE WASNMILWEF 1301 IGDDLSFIMK DVDIGVKFSE LNTDLLPIYY TMAGRILAMG IRFASTGNLK 1351 IRNILLSLVD KFLPLYQYPG KQNLDFRLTI SVINVLTNVI VVSLSMVMCA 1401 SGDLEVLRRV KYLHEVASGP YSDLFQEIPS SKSDVSGVTQ VTSNTNTPGN 1451 SDRERVDETA ASLDDERSSN GSDISDPTAY LEDKKDIDDH YGKFISTNLA 1501 LGFLFLGSGQ YALNTSTLES IAFLSMSVLP TYTTPHPLQE LKHFWSMAVE 1551 PRCLVIKDIS TGDAVNNVPI ELVVEEDVEK EEVIREISTP CLLPDFSKIK 1601 SIRVKMHGYF PLEVNFTKDY SASDFFSGGT IIYIQRKSES VFENKASFRN 1651 VEDIHVALKR KAAESKNYSR LNLKNEQGNT TSSQLVESLG IQDLTMVELD 1701 TLLSAGNNTA LTDSESYNLG LLCSDKNSGD ILDCQLELWY KSFGPHDE*

Amino Acid Sequence (or in FASTA format)

       1  MTSKPSTRND YLPRETHNGE YTGDSPEWQL QINITNKIGG INGDIWLSRD
      51  GRSVKWCIED QCLRQFTYNQ KIIKAGYIDF EKTPDCFVVV LSDIAHVYML
     101  KNGGSTTVCF PFQIGNAFWY ANGVILERET SASFMDGGYD LKPIEFDLKH
     151  KYITLTDPMA PFGLISITNT FKGGINSASG NKTDILQDFQ LVLFPSDKDK
     201  CIAVFLDRNS KVLRFYYSRI LSSDQSRKGE LTISSTKKTG LDAAGNSQKS
     251  GGISKDLRKF SHLTRRSTSI NSNSHDFNAA ERVLSGNVGN ASGRTDIFAL
     301  PSSCSRRSLS ATLDRMGNNI APTNRAAPSG FFDSSSANTA THSNITPVSQ
     351  PMQQQQQEYL NQTATSSKDI VLTEISSLKL PDDIIFTSRR LSSILSKLKF
     401  LSLRFERREG LLIFHEPTHF CKIWLIDLLP DVLDSIPFKI YGNSPQNMIR
     451  LENLKLKEPS RIQAMYIHEL LESCLILVSE GQNKEEYKAC LYDPFVKITS
     501  PSKNISEELT KQNSLPSLQK LFPYPETSFT KLCFEAVKYI TSPAFNISFI
     551  FLWQSAYSIL LSRANDDVVG GLKMEHDAFS LVLSLLILPI PSSSAQEYQE
     601  YKEIYERDLF QHLKQDSEIT SSVLPRIVIG LHLIREEYSL NVLCRNEHAL
     651  LGQFLRFATA AMGWPDLWQS YYVPKMDSES KTFLHPREQN STFFHPLDEP
     701  PSITKSLYSI TENSSIPLCP FISFSRLVAT DTQVELRITP RSFKILGLYE
     751  LVHSPNFLPD YVLGILSSFK VDKDELQTYP LGILVPLQNI LKILEDKLSE
     801  VRDNLELLDR ADLQRCSAII NSIRSDSKEV LKRGQRDSYM LCKVPLAKNR
     851  SSLSKKPSDI YSILSEIVKS ASQVPLDGSA MRMSNIQDDE DIDEGRSLKL
     901  NAGLIFSEDK RFTHVVSLLA YYRPTKTQFF TTKTEYAQIL AQKKYFAKIM
     951  ALRTCTNGVG WGAVAYATEK PISTQKWVIQ PLNLISVFPD DTKITVKAPE
    1001  DIAHDIVEWG QFHAGVSSGL RISKKATGIT GSWIAFNKPK ELDAYHGGFL
    1051  LGLGLNGHLK NLEEWHIYNY LSPRNTHISI GLLLGMSSSM KGSMDSKLIK
    1101  VISVHLVAFL PSGSSDLNID LKLQTAGIIG MGMLYLNSRH KRMSDSIFAQ
    1151  LVSLLNVNDE MVADEEYRLA AGISLGLINL GAGQTKLRKW DSSLLGLGDD
    1201  LPEDVYDSSD VEQNVMYEDL TTKLLEIVTS TYDVENDWIP ENSQIGAVIA
    1251  IMFLFLKSNN FGISNMLKVD LKEILKANIN TRPELLMYRE WASNMILWEF
    1301  IGDDLSFIMK DVDIGVKFSE LNTDLLPIYY TMAGRILAMG IRFASTGNLK
    1351  IRNILLSLVD KFLPLYQYPG KQNLDFRLTI SVINVLTNVI VVSLSMVMCA
    1401  SGDLEVLRRV KYLHEVASGP YSDLFQEIPS SKSDVSGVTQ VTSNTNTPGN
    1451  SDRERVDETA ASLDDERSSN GSDISDPTAY LEDKKDIDDH YGKFISTNLA
    1501  LGFLFLGSGQ YALNTSTLES IAFLSMSVLP TYTTPHPLQE LKHFWSMAVE
    1551  PRCLVIKDIS TGDAVNNVPI ELVVEEDVEK EEVIREISTP CLLPDFSKIK
    1601  SIRVKMHGYF PLEVNFTKDY SASDFFSGGT IIYIQRKSES VFENKASFRN
    1651  VEDIHVALKR KAAESKNYSR LNLKNEQGNT TSSQLVESLG IQDLTMVELD
    1701  TLLSAGNNTA LTDSESYNLG LLCSDKNSGD ILDCQLELWY KSFGPHDE*

external links for Apc1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Apc1p

1)	Zachariae W, et al. (1996) Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae. Science 274(5290):1201-4
2)	Zachariae W, et al. (1998) Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science 279(5354):1216-9
3)	Zachariae W and Nasmyth K (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
4)	Buschhorn BA, et al. (2011) Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nat Struct Mol Biol 18(1):6-13
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41